Pub Date : 2021-12-31DOI: 10.52419/issn2072-2419.2021.4.196
R. N. Bulatov, B. S. Avdeenko, E. M. Sengaliev, K. Plemyashov
Analysis of the results of medical examination of pregnant sheep 30...15...5 days before the expected term of lambing showed that in the urine of 12.11% of ewes, the concentration of ketone bodies in the blood plasma was 1.43 times higher than the physiological norm. In addition, there is a tendency to decrease the buffer bases to 18.41±1.53 mmol/l, as well as the glucose content below 2.12 ± 0.12 mmol/l. At the same time, the ratio of VN/ ACAS is up to 1.47 ± 0.12 mmol / L. It was found that the albumin fraction of proteins decreased by 1.51 times. Edema in the abdominal wall and underbelly, 9.5% lack of reaction to external stimuli, 15.0% decreased appetite, 10.0% jaundice of mucous membranes and 9.0% of sheep had a comatose state were found in 15.0% of animals. In a clinical study in sheep with eclampsia, hypertension over 136.1 mmHg and the presence of protein in urine over 3.2 g/l were recorded. These symptoms indicated classic signs of eclampsia, which occurred depending on the condition of sick animals in atypical and typical forms of manifestation. In animals with typical clinical signs of eclampsia, an increase in the level of diene conjugates was found to be 1.87 times, and the concentration of ketodiene intermediates and conjugated trienes was 1.75 times, the concentration of stable nitric oxide metabolites was 38.0%, and the vitamin E content decreased by 13.1%. At the same time, there was a 20.46% increase in the concentration of double bonds in the atypical form of eclampsia. In the blood plasma of patients with eclampsia of pregnant ewes, the activity of superoxide dismutase (1,736±0.37 units) is lower than in the comparison group (2,146±0.56 mmol/l). The obtained material of this work should be taken into account in the future when studying the problem of complicated pregnancy in the last stages of gestation in sheep and when developing therapeutic and preventive measures to ensure the long-term functioning of the reproductive potential and obtaining a viable newborn offspring.
{"title":"Oxidative stress in suyagny sheep at the end of gestation, as a factor in pathogenesis development of eclampsia","authors":"R. N. Bulatov, B. S. Avdeenko, E. M. Sengaliev, K. Plemyashov","doi":"10.52419/issn2072-2419.2021.4.196","DOIUrl":"https://doi.org/10.52419/issn2072-2419.2021.4.196","url":null,"abstract":"Analysis of the results of medical examination of pregnant sheep 30...15...5 days before the expected term of lambing showed that in the urine of 12.11% of ewes, the concentration of ketone bodies in the blood plasma was 1.43 times higher than the physiological norm. In addition, there is a tendency to decrease the buffer bases to 18.41±1.53 mmol/l, as well as the glucose content below 2.12 ± 0.12 mmol/l. At the same time, the ratio of VN/ ACAS is up to 1.47 ± 0.12 mmol / L. It was found that the albumin fraction of proteins decreased by 1.51 times. Edema in the abdominal wall and underbelly, 9.5% lack of reaction to external stimuli, 15.0% decreased appetite, 10.0% jaundice of mucous membranes and 9.0% of sheep had a comatose state were found in 15.0% of animals. In a clinical study in sheep with eclampsia, hypertension over 136.1 mmHg and the presence of protein in urine over 3.2 g/l were recorded. These symptoms indicated classic signs of eclampsia, which occurred depending on the condition of sick animals in atypical and typical forms of manifestation. In animals with typical clinical signs of eclampsia, an increase in the level of diene conjugates was found to be 1.87 times, and the concentration of ketodiene intermediates and conjugated trienes was 1.75 times, the concentration of stable nitric oxide metabolites was 38.0%, and the vitamin E content decreased by 13.1%. At the same time, there was a 20.46% increase in the concentration of double bonds in the atypical form of eclampsia. In the blood plasma of patients with eclampsia of pregnant ewes, the activity of superoxide dismutase (1,736±0.37 units) is lower than in the comparison group (2,146±0.56 mmol/l). The obtained material of this work should be taken into account in the future when studying the problem of complicated pregnancy in the last stages of gestation in sheep and when developing therapeutic and preventive measures to ensure the long-term functioning of the reproductive potential and obtaining a viable newborn offspring.","PeriodicalId":378052,"journal":{"name":"International bulletin of Veterinary Medicine","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125606388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-31DOI: 10.52419/issn2072-2419.2021.4.229
O. Kryachko
The international scientific conference was dedicated to the 100th anniversary of the Department of Pathological Physiology of the St. Petersburg state university of veterinary medicine. The audience was presented with a historical reference about the department and its first head – prof. London E.S., also scientific schools were characterized and an idea of the current state of the department was given. The conference discussed issues of general etiology and general pathogenesis of animal diseases, pathogenetic foundations of non-infectious diseases, considered various experimental models in pathology and paid attention to the discussion of teaching pathophysiology and the introduction of digital technologies in the educational process in modern conditions. The holding of the event in the format of Zoom video conferences with the broadcast of meetings on YouTube gave an opportunity to participate as a listener to a wide range of interested persons.
{"title":"About the conference dedicated to the 100th anniversary of the department of pathological physiology St. Petersburg state university of veterinary medicine","authors":"O. Kryachko","doi":"10.52419/issn2072-2419.2021.4.229","DOIUrl":"https://doi.org/10.52419/issn2072-2419.2021.4.229","url":null,"abstract":"The international scientific conference was dedicated to the 100th anniversary of the Department of Pathological Physiology of the St. Petersburg state university of veterinary medicine. The audience was presented with a historical reference about the department and its first head – prof. London E.S., also scientific schools were characterized and an idea of the current state of the department was given. The conference discussed issues of general etiology and general pathogenesis of animal diseases, pathogenetic foundations of non-infectious diseases, considered various experimental models in pathology and paid attention to the discussion of teaching pathophysiology and the introduction of digital technologies in the educational process in modern conditions. The holding of the event in the format of Zoom video conferences with the broadcast of meetings on YouTube gave an opportunity to participate as a listener to a wide range of interested persons.","PeriodicalId":378052,"journal":{"name":"International bulletin of Veterinary Medicine","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123736948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-31DOI: 10.52419/issn2072-2419.2021.4.206
A. Trunov, R. R. Kadyrov, V. Videnin
The paper presents the results of a clinical and experimental study of determining the clinical effectiveness of surgical treatment of animals with patent ductus arteriosus using open ligation and endovascular occlusion using the example of dogs of the Corgi and Spitz breeds. A retrospective study, the morphofunctional state of the cardiovascular system was assessed before and after surgery. The total sample consisted of 25 animals. Diagnosis of this defect was carried out by auscultation and using echocardiography [ultrasound]. During auscultation, a systolic-diastolic murmur is heard. On ultrasound, the normalized parameters were compared before and after surgery. Animals with critical pulmonary hypertension were excluded from the study, since this complication is a contraindication for surgical treatment. In the postoperative period, the animals after open correction of the defect recovered longer and needed more prolonged analgesia than animals after endovascular treatment. After open treatment with thoracotomy, the animals were in the intensive care unit for a day, and after transcatheter surgery - 6-12 hours. After that, all animals were discharged for outpatient treatment. After closure of the duct, all dogs with a congenital defect became more active, their general condition improved, shortness of breath during physical exertion disappeared, and the respiratory rate decreased significantly. It was found that corgi are more difficult to tolerate this defect than Spitz dogs. They had a more frequent complication in the form of pulmonary hypertension. One year after the surgical treatment, when performing echocardiography, the indicators of the morphofunctional state of the heart were normalized.
{"title":"Diagnosis and surgical treatment open ductus arteriosus in corgi and pomeranian dogs","authors":"A. Trunov, R. R. Kadyrov, V. Videnin","doi":"10.52419/issn2072-2419.2021.4.206","DOIUrl":"https://doi.org/10.52419/issn2072-2419.2021.4.206","url":null,"abstract":"The paper presents the results of a clinical and experimental study of determining the clinical effectiveness of surgical treatment of animals with patent ductus arteriosus using open ligation and endovascular occlusion using the example of dogs of the Corgi and Spitz breeds. A retrospective study, the morphofunctional state of the cardiovascular system was assessed before and after surgery. The total sample consisted of 25 animals. Diagnosis of this defect was carried out by auscultation and using echocardiography [ultrasound]. During auscultation, a systolic-diastolic murmur is heard. On ultrasound, the normalized parameters were compared before and after surgery. Animals with critical pulmonary hypertension were excluded from the study, since this complication is a contraindication for surgical treatment. In the postoperative period, the animals after open correction of the defect recovered longer and needed more prolonged analgesia than animals after endovascular treatment. After open treatment with thoracotomy, the animals were in the intensive care unit for a day, and after transcatheter surgery - 6-12 hours. After that, all animals were discharged for outpatient treatment. After closure of the duct, all dogs with a congenital defect became more active, their general condition improved, shortness of breath during physical exertion disappeared, and the respiratory rate decreased significantly. It was found that corgi are more difficult to tolerate this defect than Spitz dogs. They had a more frequent complication in the form of pulmonary hypertension. One year after the surgical treatment, when performing echocardiography, the indicators of the morphofunctional state of the heart were normalized.","PeriodicalId":378052,"journal":{"name":"International bulletin of Veterinary Medicine","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126555654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-31DOI: 10.52419/issn2072-2419.2021.4.213
M. O. Voronova, Y. Vatnikov
The objective of this study was to compare three different local anesthetics in terms of duration and effectiveness of block development in brachial plexus block for osteosynthesis of radius with plate in dogs. The blockade was received a electroneurostimulation-guided Stimuplex HNS 12 with SENSe function by B. Brown for all group. 15 dogs of smallest breeds were divided into three random groups: in the first, 2% lidocaine solution was used as anesthesia; in the second bupivacaine 0.5%, in the third ropivacaine 0.5%. The time to development and the duration of the local block were assessed with a pinch method in several areas. The fastest development of a complete limb block was observed in group 1, which took an average of 7-10 minutes. The development of a complete block in the 2nd group exceeded three times, and in the 3rd - twice, the anesthetic indicators of the 1st group. All patients from group 1 were immediately injected with analgesics after surgery to relieve postoperative pain. The duration of the blockade of the third group was significantly shorter than in the second group, however, the motor block in the second group was significantly longer than in the 3rd group, patients experienced pain, but the motor function of the distal limb has not yet recovered. Thus, ropivacaine, by its properties, is the drug of choice for brachial plexus blockade for periosteal osteosynthesis of the forelimb in dogs and can be used for surgeries longer than 80 minutes.
{"title":"Comparative characteristics of local anesthetics in brachial plexus block for surgery in dogs","authors":"M. O. Voronova, Y. Vatnikov","doi":"10.52419/issn2072-2419.2021.4.213","DOIUrl":"https://doi.org/10.52419/issn2072-2419.2021.4.213","url":null,"abstract":"The objective of this study was to compare three different local anesthetics in terms of duration and effectiveness of block development in brachial plexus block for osteosynthesis of radius with plate in dogs. The blockade was received a electroneurostimulation-guided Stimuplex HNS 12 with SENSe function by B. Brown for all group. 15 dogs of smallest breeds were divided into three random groups: in the first, 2% lidocaine solution was used as anesthesia; in the second bupivacaine 0.5%, in the third ropivacaine 0.5%. The time to development and the duration of the local block were assessed with a pinch method in several areas. The fastest development of a complete limb block was observed in group 1, which took an average of 7-10 minutes. The development of a complete block in the 2nd group exceeded three times, and in the 3rd - twice, the anesthetic indicators of the 1st group. All patients from group 1 were immediately injected with analgesics after surgery to relieve postoperative pain. The duration of the blockade of the third group was significantly shorter than in the second group, however, the motor block in the second group was significantly longer than in the 3rd group, patients experienced pain, but the motor function of the distal limb has not yet recovered. Thus, ropivacaine, by its properties, is the drug of choice for brachial plexus blockade for periosteal osteosynthesis of the forelimb in dogs and can be used for surgeries longer than 80 minutes.","PeriodicalId":378052,"journal":{"name":"International bulletin of Veterinary Medicine","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123969349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-31DOI: 10.52419/issn2072-2419.2021.4.172
O. Kryachko, L. Lukoyanova, V. Gaponova
The study was conducted on 15 dogs aged 1.5-12 years, kept in urban conditions with symptoms of chronic stress caused by various factors: moving to a new place of residence, fear of loneliness, loss of the owner, exhibition career, active breeding activities. The drug Klim Peter was given to all animals for 21 days at a dosage of 1 tablet per 10 kg of live weight 1 time per day. Blood samples were taken for morphological and biochemical studies before the start of the study and after the use of the drug (on the 24th day from the start of the experiment). The drug contributed to a significant reduction in the initially elevated level of white blood cells, the number of red blood cells and the concentration of hemoglobin, a decrease in ESR. The ratio of white blood cells in the leukogram approached the standard values. Protein and albumin levels were significantly increased.The normalization of anabolic processes was confirmed by a decrease in urea and creatinine levels, as well as a significant decrease in the level of bilirubin and the activity of enzymes – ALT, AST and alkaline phosphatase. After 5-10 days after the start of taking the drug, the animals showed normalization of appetite and digestion, after 14-18 days, neurological symptoms were leveled (destructive behavior, tremor, unmotivated aggression, obsessive behavior). By the 20th day, there was an improvement in the condition of the skin and coat, molting in most stopped or decreased, the coat became thick, shiny. Thus, the use of the drug Klim Pet had a positive effect on the clinical condition, and also helped to neutralize the effects of stress factors of various etiologies, had a hepatoprotective effect, optimized the metabolism and activity of the immune and nervous systems, contributed to improving the condition of the fur and skin.
{"title":"Correction of the functional state of the regulatory systems of the body of dogs under the influence of environmental stress factors","authors":"O. Kryachko, L. Lukoyanova, V. Gaponova","doi":"10.52419/issn2072-2419.2021.4.172","DOIUrl":"https://doi.org/10.52419/issn2072-2419.2021.4.172","url":null,"abstract":"The study was conducted on 15 dogs aged 1.5-12 years, kept in urban conditions with symptoms of chronic stress caused by various factors: moving to a new place of residence, fear of loneliness, loss of the owner, exhibition career, active breeding activities. The drug Klim Peter was given to all animals for 21 days at a dosage of 1 tablet per 10 kg of live weight 1 time per day. Blood samples were taken for morphological and biochemical studies before the start of the study and after the use of the drug (on the 24th day from the start of the experiment). The drug contributed to a significant reduction in the initially elevated level of white blood cells, the number of red blood cells and the concentration of hemoglobin, a decrease in ESR. The ratio of white blood cells in the leukogram approached the standard values. Protein and albumin levels were significantly increased.The normalization of anabolic processes was confirmed by a decrease in urea and creatinine levels, as well as a significant decrease in the level of bilirubin and the activity of enzymes – ALT, AST and alkaline phosphatase. After 5-10 days after the start of taking the drug, the animals showed normalization of appetite and digestion, after 14-18 days, neurological symptoms were leveled (destructive behavior, tremor, unmotivated aggression, obsessive behavior). By the 20th day, there was an improvement in the condition of the skin and coat, molting in most stopped or decreased, the coat became thick, shiny. Thus, the use of the drug Klim Pet had a positive effect on the clinical condition, and also helped to neutralize the effects of stress factors of various etiologies, had a hepatoprotective effect, optimized the metabolism and activity of the immune and nervous systems, contributed to improving the condition of the fur and skin.","PeriodicalId":378052,"journal":{"name":"International bulletin of Veterinary Medicine","volume":"126 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122855113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-31DOI: 10.52419/issn2072-2419.2021.4.219
G. Shiryaev
Purpose: The aim of the research is to study the effect of subclinical ketosis (SCK) on the effectiveness of artificial insemination of highly productive dairy cows in the postpartum period.Materials and methods. The studied animals were selected according to the principle of conditional analogs and were divided into 2 groups of 6 animals each. Group 1 - animals with a blood concentration of 3- hydroxybutyrate˂ 1.0 mmol / l, group 2 - animals with subclinical ketosis with a concentration of 3-hydroxybutyrate in the blood in the range of 1.0-1.4 mmol / l. Blood was collected from animals on the 5th, 15th and 33-35th days after calving. In the obtained blood serum samples, the content of total protein, albumin, urea, glucose, cholesterol, triglycerides, calcium, phosphorus, magnesium, creatinine, total bilirubin, the activity of transaminase enzymes and alkaline phosphatase was determined. The concentration of steroid hormones has been determined. Before artificial insemination, the animals were synchronized according to the pressinovsinh scheme.Results. Most of the biochemical blood parameters did not go beyond the reference values. By the time of hormonal stimulation of sexual heat in cows with SCK, a decrease in 3-hydroxybutyrate (˂ 1,0 mmol / l) and an increase in glucose (> 3.0 mmol / l) were recorded. On the 15th day after calving, in the group with SCK, there was an increase in total bilirubin, cholesterol, triglycerides, and magnesium (p˂ 0,01). On the 15th day after calving, testosterone levels were 13% higher in the SCK group. By the time of synchronization, the level of progesterone in the group of animals with SCK was lower in comparison with the control group. In the group of animals with SCK, the lowest reproductive indices were recorded: the insemination index in the group with SSC was higher by 46%, the service period was 49 days longer, the interbody period was significantly longer by 68 days (p˂ 0,05).Conclusion. Subclinical ketosis, recorded on the 15th day after calving, helps to reduce the reproductive performance of high -yielding dairy cows. Normalization of the concentration of 3-hydroxybutyrate from subclinical to physiologically normal values, and subsequent hormonal synchronization do not reduce this negative effect of SCK.
{"title":"Subclinical ketosis as a factor reduction of reproductive indicators of high productive cows","authors":"G. Shiryaev","doi":"10.52419/issn2072-2419.2021.4.219","DOIUrl":"https://doi.org/10.52419/issn2072-2419.2021.4.219","url":null,"abstract":"Purpose: The aim of the research is to study the effect of subclinical ketosis (SCK) on the effectiveness of artificial insemination of highly productive dairy cows in the postpartum period.Materials and methods. The studied animals were selected according to the principle of conditional analogs and were divided into 2 groups of 6 animals each. Group 1 - animals with a blood concentration of 3- hydroxybutyrate˂ 1.0 mmol / l, group 2 - animals with subclinical ketosis with a concentration of 3-hydroxybutyrate in the blood in the range of 1.0-1.4 mmol / l. Blood was collected from animals on the 5th, 15th and 33-35th days after calving. In the obtained blood serum samples, the content of total protein, albumin, urea, glucose, cholesterol, triglycerides, calcium, phosphorus, magnesium, creatinine, total bilirubin, the activity of transaminase enzymes and alkaline phosphatase was determined. The concentration of steroid hormones has been determined. Before artificial insemination, the animals were synchronized according to the pressinovsinh scheme.Results. Most of the biochemical blood parameters did not go beyond the reference values. By the time of hormonal stimulation of sexual heat in cows with SCK, a decrease in 3-hydroxybutyrate (˂ 1,0 mmol / l) and an increase in glucose (> 3.0 mmol / l) were recorded. On the 15th day after calving, in the group with SCK, there was an increase in total bilirubin, cholesterol, triglycerides, and magnesium (p˂ 0,01). On the 15th day after calving, testosterone levels were 13% higher in the SCK group. By the time of synchronization, the level of progesterone in the group of animals with SCK was lower in comparison with the control group. In the group of animals with SCK, the lowest reproductive indices were recorded: the insemination index in the group with SSC was higher by 46%, the service period was 49 days longer, the interbody period was significantly longer by 68 days (p˂ 0,05).Conclusion. Subclinical ketosis, recorded on the 15th day after calving, helps to reduce the reproductive performance of high -yielding dairy cows. Normalization of the concentration of 3-hydroxybutyrate from subclinical to physiologically normal values, and subsequent hormonal synchronization do not reduce this negative effect of SCK.","PeriodicalId":378052,"journal":{"name":"International bulletin of Veterinary Medicine","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125610039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-31DOI: 10.52419/issn2072-2419.2021.4.166
A. Stekolnikov, L. Karpenko, N. A. Shinkarevich, A. Bakhta, A. I. Kozitsyna
In the agro-industrial complex livestock sector the tendency to reduce the use of antibacterial agents in production is increasing. In this regard, the use of probiotics is gaining popularity around the world. Being antagonists of pathogenic and opportunistic intestinal microorganisms, suppressing their growth and reproduction, as well as creating favorable conditions for the obligate microflora of the gastrointestinal tract, probiotics are becoming increasingly important. The feed biologically active additive "Vetlaktoflor" belongs to the preparations of this group.The purpose of the presented work was to study the effect of the feed biologically active additive "Vetlaktoflor" on the biochemical parameters of the blood of pregnant pigs. To study the effect of the drug, 4 groups of sows were formed. One control group without the use of the drug and 3 experimental groups where the drug was used according to the following scheme: experimental group-2 - animals were given the active additive "Vetlaktoflor" 2 weeks before farrowing, experimental group-3 - animals were given the active additive "Vetlaktoflor" 2 months before farrowing, experimental group-4 - animals received the biologically active additive "Vetlaktoflor" twice during pregnancy: 2 months and 2 weeks before farrowing.As a result of the use of a biologically active additive, stable values of total protein and its fractions, urea, creatinine were noted. At the same time, the increase in total protein values increased in the range from 16.12% to 17.01%, albumin values increased to 62.10%, globulin values increased to 34.96%. Indicators of urea and creatinine with a slight increase remained within the reference values. Thus, the probiotic "Vetlaktoflor" is effective when used during pregnancy, stimulating the processes of protein metabolism, thereby ensuring a stable course of pregnancy. With intensive nitrogen metabolism characteristic of pregnancy, this biologically active additive ensures that urea and creatinine indicators are found within the average reference values.
{"title":"Protein metabolism activation in pig industry in pregnant sows","authors":"A. Stekolnikov, L. Karpenko, N. A. Shinkarevich, A. Bakhta, A. I. Kozitsyna","doi":"10.52419/issn2072-2419.2021.4.166","DOIUrl":"https://doi.org/10.52419/issn2072-2419.2021.4.166","url":null,"abstract":"In the agro-industrial complex livestock sector the tendency to reduce the use of antibacterial agents in production is increasing. In this regard, the use of probiotics is gaining popularity around the world. Being antagonists of pathogenic and opportunistic intestinal microorganisms, suppressing their growth and reproduction, as well as creating favorable conditions for the obligate microflora of the gastrointestinal tract, probiotics are becoming increasingly important. The feed biologically active additive \"Vetlaktoflor\" belongs to the preparations of this group.The purpose of the presented work was to study the effect of the feed biologically active additive \"Vetlaktoflor\" on the biochemical parameters of the blood of pregnant pigs. To study the effect of the drug, 4 groups of sows were formed. One control group without the use of the drug and 3 experimental groups where the drug was used according to the following scheme: experimental group-2 - animals were given the active additive \"Vetlaktoflor\" 2 weeks before farrowing, experimental group-3 - animals were given the active additive \"Vetlaktoflor\" 2 months before farrowing, experimental group-4 - animals received the biologically active additive \"Vetlaktoflor\" twice during pregnancy: 2 months and 2 weeks before farrowing.As a result of the use of a biologically active additive, stable values of total protein and its fractions, urea, creatinine were noted. At the same time, the increase in total protein values increased in the range from 16.12% to 17.01%, albumin values increased to 62.10%, globulin values increased to 34.96%. Indicators of urea and creatinine with a slight increase remained within the reference values. Thus, the probiotic \"Vetlaktoflor\" is effective when used during pregnancy, stimulating the processes of protein metabolism, thereby ensuring a stable course of pregnancy. With intensive nitrogen metabolism characteristic of pregnancy, this biologically active additive ensures that urea and creatinine indicators are found within the average reference values.","PeriodicalId":378052,"journal":{"name":"International bulletin of Veterinary Medicine","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131317568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-31DOI: 10.52419/issn2072-2419.2021.4.177
V. Reshetnyak, A. Stekolnikov, V. Burdeyniy, L. Malakhova
Pig breeding is one of the leading farm industries for the production of meat products. Castration is one of the techniques that help to increase the productivity of animals and regulate the quality of meat (eliminating the “smell of boar meat”). Its disadvantage is the presence of post-castration complications. An effective method of reducing their number is the use of drugs that have an immunotropic effect.Therefore, we conducted an experiment to find out the effect of one of this drug group representatives (timogen) on the dynamics of hematological parameters during castration of boars.The data of hematological studies (during the experiment) signified a violation of homeostasis, manifested in the first fourteen days after castration by the development of anemia (a decrease in the number of erythrocytes and hemoglobin) and thrombocytopenia, which (like erythropenia) was recorded against the background of anisocytosis. At the same time, the results, reflecting the picture of white blood, indicated an inflammatory process accompanied by leukocytosis, due to both the absolute and the relative number of lymphocytes, as well as the absolute number of medium-sized cells (MID). On the 30th day of observation, an increase in the number of erythrocytes and hemoglobin relative to the 14th day was noted against the background of a decrease in platelets and leukopenia.Thus, immunocorrection with a five-day course of timogen made it possible to neutralize the negative effect of surgical intervention on hematopoiesis, which allowed us to avoid sharp fluctuations in the morphological composition of red and white blood.
{"title":"Dynamics of hematological parameters during castration of boars on the background of immunocorrection","authors":"V. Reshetnyak, A. Stekolnikov, V. Burdeyniy, L. Malakhova","doi":"10.52419/issn2072-2419.2021.4.177","DOIUrl":"https://doi.org/10.52419/issn2072-2419.2021.4.177","url":null,"abstract":"Pig breeding is one of the leading farm industries for the production of meat products. Castration is one of the techniques that help to increase the productivity of animals and regulate the quality of meat (eliminating the “smell of boar meat”). Its disadvantage is the presence of post-castration complications. An effective method of reducing their number is the use of drugs that have an immunotropic effect.Therefore, we conducted an experiment to find out the effect of one of this drug group representatives (timogen) on the dynamics of hematological parameters during castration of boars.The data of hematological studies (during the experiment) signified a violation of homeostasis, manifested in the first fourteen days after castration by the development of anemia (a decrease in the number of erythrocytes and hemoglobin) and thrombocytopenia, which (like erythropenia) was recorded against the background of anisocytosis. At the same time, the results, reflecting the picture of white blood, indicated an inflammatory process accompanied by leukocytosis, due to both the absolute and the relative number of lymphocytes, as well as the absolute number of medium-sized cells (MID). On the 30th day of observation, an increase in the number of erythrocytes and hemoglobin relative to the 14th day was noted against the background of a decrease in platelets and leukopenia.Thus, immunocorrection with a five-day course of timogen made it possible to neutralize the negative effect of surgical intervention on hematopoiesis, which allowed us to avoid sharp fluctuations in the morphological composition of red and white blood.","PeriodicalId":378052,"journal":{"name":"International bulletin of Veterinary Medicine","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131733769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-30DOI: 10.52419/issn2072-2419.2021.4.141
V. Khvatov, M. Shchipakin, N. Zelenevsky, D. Bylinskaya
The study of the features of the morphology of the heart and its structures in farm animals in species and breed aspects is an urgent direction in fundamental research. Anglo-Nubian goats are a versatile goat breed, and currently their breeding is gaining popularity in the North-West region of the Russian Federation. Due to the capriciousness of this breed of goats to cold temperatures, they can often suffer from diseases of the cardiovascular and respiratory systems. The purpose of our study is to establish the histological features of the structure of the interventricular septum of the heart of an Anglo-Nubian goat.Eleven corpses of Anglo-Nubian goats obtained during slaughter in the farm of the Moscow region of the Russian Federation "Gzhelpodvorie" were presented as the material under study. The age of the corpses of Anglo-Nubian goats was 12-13 months. As research methods were used: fine anatomical preparation and manufacture of histological preparations.We found that the composition of the interventricular septum of the heart of an Anglo-Nubian goat contains contractile cardiomyocytes, strands of loose unformed connective tissue, muscle and mixed blood vessels, nerve trunks, a few Purkinje fibers, as well as the endocardium. Also established morphometric data of histostructures of the interventricular septum in a goat of the Anglo-Nubian breed.
{"title":"Regularities of the histological structure of the interventricular septum of the heart of a goat of the anglonubian breed","authors":"V. Khvatov, M. Shchipakin, N. Zelenevsky, D. Bylinskaya","doi":"10.52419/issn2072-2419.2021.4.141","DOIUrl":"https://doi.org/10.52419/issn2072-2419.2021.4.141","url":null,"abstract":"The study of the features of the morphology of the heart and its structures in farm animals in species and breed aspects is an urgent direction in fundamental research. Anglo-Nubian goats are a versatile goat breed, and currently their breeding is gaining popularity in the North-West region of the Russian Federation. Due to the capriciousness of this breed of goats to cold temperatures, they can often suffer from diseases of the cardiovascular and respiratory systems. The purpose of our study is to establish the histological features of the structure of the interventricular septum of the heart of an Anglo-Nubian goat.Eleven corpses of Anglo-Nubian goats obtained during slaughter in the farm of the Moscow region of the Russian Federation \"Gzhelpodvorie\" were presented as the material under study. The age of the corpses of Anglo-Nubian goats was 12-13 months. As research methods were used: fine anatomical preparation and manufacture of histological preparations.We found that the composition of the interventricular septum of the heart of an Anglo-Nubian goat contains contractile cardiomyocytes, strands of loose unformed connective tissue, muscle and mixed blood vessels, nerve trunks, a few Purkinje fibers, as well as the endocardium. Also established morphometric data of histostructures of the interventricular septum in a goat of the Anglo-Nubian breed.","PeriodicalId":378052,"journal":{"name":"International bulletin of Veterinary Medicine","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125364231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-12-30DOI: 10.52419/issn2072-2419.2021.4.97
T. Larkina, A. Krutikova, G. Peglivanyan, N. V. Dementiev
Obesity of chickens significantly reduces the efficiency of feeding, egg production, palatability of meat carcasses, slaughter yield, consumer value, and there is also such an ecological aspect as the problem of fat utilization. The search for SNPs in genes involved in lipid metabolism is an important task today for modern research in the field of poultry farming. Differences in carcass fat between breeds and lines within a breed illustrate the importance of genetic factors in lipid metabolism in chickens. The aim of the study was to find and analyze SNPs in the PPARG gene by sequencing in various chicken breeds (n = 83). The objects of the study were chickens from the VNIIGZh bioresource collection "Genetic collection of rare and endangered chicken breeds" (Pushkin, St. Petersburg) of various productivity trends (n = 83). Blood for DNA isolation was taken from hens at the age of 330 days from a wing vein into a microtube containing 50 μl of 0.5 mM EDTA as an anticoagulant. Prior to use, blood samples were stored at -200C. Genomic DNA was isolated using a standard phenolic-detergent method. The concentration and purity of the samples were determined using a NanoDrop 2000 instrument (Thermo Fisher, USA).Analysis of the polymorphism of the regulatory region of the PPARG gene was carried out by sequencing. The primer design was carried out on the basis of information from the Internet database.The studies were carried out on the basis of the laboratory of molecular genetics of the RRIFAGB. Genetic variability for rs314476701 of the PPARG gene was determined in the analyzed breeds. SNP rs316237745 revealed a strong shift of the A.
鸡的肥胖显著降低了饲养效率、产蛋率、胴体的适口性、屠宰产量、消费价值，还存在着脂肪利用等生态方面的问题。寻找与脂质代谢有关的基因的snp是当今家禽养殖领域现代研究的一项重要任务。不同品种和品种间胴体脂肪的差异说明了遗传因素在鸡脂质代谢中的重要性。本研究的目的是通过测序在各种鸡品种(n = 83)中发现和分析PPARG基因的snp。研究对象为VNIIGZh生物资源库“珍稀和濒危鸡种遗传集”(Pushkin, St. Petersburg)中各种生产力趋势的鸡(n = 83)。取330日龄鸡翅膀静脉的血液，放入含有50 μl 0.5 mM EDTA抗凝剂的微管中进行DNA分离。使用前，血样保存在-200℃。基因组DNA采用标准的酚醛洗涤剂方法分离。样品的浓度和纯度使用NanoDrop 2000仪器(Thermo Fisher, USA)测定。通过测序对PPARG基因调控区进行多态性分析。引子设计是根据Internet数据库的信息进行的。研究是在RRIFAGB分子遗传学实验室的基础上进行的。测定了PPARG基因rs314476701的遗传变异性。SNP rs316237745显示了a的强烈移位。
{"title":"Genetic variability of genetic chicken breeds estimated based on SNPS analysis in the PPARG gene","authors":"T. Larkina, A. Krutikova, G. Peglivanyan, N. V. Dementiev","doi":"10.52419/issn2072-2419.2021.4.97","DOIUrl":"https://doi.org/10.52419/issn2072-2419.2021.4.97","url":null,"abstract":"Obesity of chickens significantly reduces the efficiency of feeding, egg production, palatability of meat carcasses, slaughter yield, consumer value, and there is also such an ecological aspect as the problem of fat utilization. The search for SNPs in genes involved in lipid metabolism is an important task today for modern research in the field of poultry farming. Differences in carcass fat between breeds and lines within a breed illustrate the importance of genetic factors in lipid metabolism in chickens. The aim of the study was to find and analyze SNPs in the PPARG gene by sequencing in various chicken breeds (n = 83). The objects of the study were chickens from the VNIIGZh bioresource collection \"Genetic collection of rare and endangered chicken breeds\" (Pushkin, St. Petersburg) of various productivity trends (n = 83). Blood for DNA isolation was taken from hens at the age of 330 days from a wing vein into a microtube containing 50 μl of 0.5 mM EDTA as an anticoagulant. Prior to use, blood samples were stored at -200C. Genomic DNA was isolated using a standard phenolic-detergent method. The concentration and purity of the samples were determined using a NanoDrop 2000 instrument (Thermo Fisher, USA).Analysis of the polymorphism of the regulatory region of the PPARG gene was carried out by sequencing. The primer design was carried out on the basis of information from the Internet database.The studies were carried out on the basis of the laboratory of molecular genetics of the RRIFAGB. Genetic variability for rs314476701 of the PPARG gene was determined in the analyzed breeds. SNP rs316237745 revealed a strong shift of the A.","PeriodicalId":378052,"journal":{"name":"International bulletin of Veterinary Medicine","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128431194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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