大麦叶绿体突变体(cpm)突变体,质体变异性的一个特殊来源。

A. Landau, F. Lencina, M. Petterson, M. G. Pacheco, S. Costoya, Vanina Brizuela, A. Prina
{"title":"大麦叶绿体突变体(cpm)突变体,质体变异性的一个特殊来源。","authors":"A. Landau, F. Lencina, M. Petterson, M. G. Pacheco, S. Costoya, Vanina Brizuela, A. Prina","doi":"10.1079/9781789249095.0027","DOIUrl":null,"url":null,"abstract":"Abstract\n The plastome is usually considered a highly conserved genome. Compared with the nuclear genome, it is small and has different genetic rules. Through different molecular methods (TILLING, candidate gene sequencing, amplicon massive sequencing and plastome re-sequencing) applied to barley chloroplast mutator (cpm) seedlings, we detected more than 60 polymorphisms affecting a wide variety of plastid genes and several intergenic regions. The genes affected belonged mostly to the plastid genetic machinery and the photosynthetic apparatus, but there were also genes like matK, whose functions are so far not clearly established. Among the isolated mutants, we found the first infA gene mutant in higher plants, two mutants in ycf3 locus and the first psbA gene mutant in barley. The latter is used in breeding barley cultivars where PSII is tolerant to toxic herbicides. Most of the molecular changes were substitutions, and small indels located in microsatellites. However, particular combinations of polymorphisms observed in the rpl23 gene and pseudogene suggest that, besides an increased rate of mutations, an augmented rate of illegitimate recombination also occurred. Although a few substitutions were observed in the mitochondria of cpm plants, we have not yet determined the implications of the cpm for mitochondrial stability. The spectrum of plastome polymorphisms highly suggests that the cpm gene is involved in plastid DNA repair, more precisely taking part in the mismatch repair system. All results show that the cpm mutant is an extraordinary source of plastome variability for plant research and/or plant breeding. This mutant also provides an interesting experimental system in which to investigate the mechanisms responsible for maintaining plastid stability.","PeriodicalId":287197,"journal":{"name":"Mutation breeding, genetic diversity and crop adaptation to climate change","volume":"1 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The barley chloroplast mutator (cpm) mutant, an extraordinary source of plastome variability.\",\"authors\":\"A. Landau, F. Lencina, M. Petterson, M. G. Pacheco, S. Costoya, Vanina Brizuela, A. Prina\",\"doi\":\"10.1079/9781789249095.0027\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract\\n The plastome is usually considered a highly conserved genome. Compared with the nuclear genome, it is small and has different genetic rules. Through different molecular methods (TILLING, candidate gene sequencing, amplicon massive sequencing and plastome re-sequencing) applied to barley chloroplast mutator (cpm) seedlings, we detected more than 60 polymorphisms affecting a wide variety of plastid genes and several intergenic regions. The genes affected belonged mostly to the plastid genetic machinery and the photosynthetic apparatus, but there were also genes like matK, whose functions are so far not clearly established. Among the isolated mutants, we found the first infA gene mutant in higher plants, two mutants in ycf3 locus and the first psbA gene mutant in barley. The latter is used in breeding barley cultivars where PSII is tolerant to toxic herbicides. Most of the molecular changes were substitutions, and small indels located in microsatellites. However, particular combinations of polymorphisms observed in the rpl23 gene and pseudogene suggest that, besides an increased rate of mutations, an augmented rate of illegitimate recombination also occurred. Although a few substitutions were observed in the mitochondria of cpm plants, we have not yet determined the implications of the cpm for mitochondrial stability. The spectrum of plastome polymorphisms highly suggests that the cpm gene is involved in plastid DNA repair, more precisely taking part in the mismatch repair system. All results show that the cpm mutant is an extraordinary source of plastome variability for plant research and/or plant breeding. This mutant also provides an interesting experimental system in which to investigate the mechanisms responsible for maintaining plastid stability.\",\"PeriodicalId\":287197,\"journal\":{\"name\":\"Mutation breeding, genetic diversity and crop adaptation to climate change\",\"volume\":\"1 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1900-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mutation breeding, genetic diversity and crop adaptation to climate change\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1079/9781789249095.0027\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mutation breeding, genetic diversity and crop adaptation to climate change","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1079/9781789249095.0027","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

质体通常被认为是高度保守的基因组。与核基因组相比,它体积小,遗传规律不同。通过对大麦叶绿体突变体(cpm)幼苗进行TILLING、候选基因测序、扩增子大规模测序和质体重测序等不同的分子方法,我们检测到60多个影响多种质体基因和多个基因间区域的多态性。受影响的基因主要属于质体遗传机制和光合机构,但也有像matK这样的基因,其功能迄今尚未明确确定。在分离的突变体中,我们发现了高等植物中第一个infA基因突变体,在ycf3位点发现了两个突变体,在大麦中发现了第一个psbA基因突变体。后者用于培育大麦品种,其中PSII耐有毒除草剂。分子变化多为取代,微卫星上有小的indel。然而,在rpl23基因和假基因中观察到的特殊多态性组合表明,除了突变率增加外,非法重组率也增加了。虽然在cpm植物的线粒体中观察到一些替代,但我们尚未确定cpm对线粒体稳定性的影响。质体多态谱高度提示cpm基因参与了质体DNA修复,更准确地说是参与错配修复系统。所有结果表明,cpm突变体是植物研究和/或植物育种中质体变异的一个非凡来源。这种突变体也提供了一个有趣的实验系统,用于研究维持质体稳定性的机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
The barley chloroplast mutator (cpm) mutant, an extraordinary source of plastome variability.
Abstract The plastome is usually considered a highly conserved genome. Compared with the nuclear genome, it is small and has different genetic rules. Through different molecular methods (TILLING, candidate gene sequencing, amplicon massive sequencing and plastome re-sequencing) applied to barley chloroplast mutator (cpm) seedlings, we detected more than 60 polymorphisms affecting a wide variety of plastid genes and several intergenic regions. The genes affected belonged mostly to the plastid genetic machinery and the photosynthetic apparatus, but there were also genes like matK, whose functions are so far not clearly established. Among the isolated mutants, we found the first infA gene mutant in higher plants, two mutants in ycf3 locus and the first psbA gene mutant in barley. The latter is used in breeding barley cultivars where PSII is tolerant to toxic herbicides. Most of the molecular changes were substitutions, and small indels located in microsatellites. However, particular combinations of polymorphisms observed in the rpl23 gene and pseudogene suggest that, besides an increased rate of mutations, an augmented rate of illegitimate recombination also occurred. Although a few substitutions were observed in the mitochondria of cpm plants, we have not yet determined the implications of the cpm for mitochondrial stability. The spectrum of plastome polymorphisms highly suggests that the cpm gene is involved in plastid DNA repair, more precisely taking part in the mismatch repair system. All results show that the cpm mutant is an extraordinary source of plastome variability for plant research and/or plant breeding. This mutant also provides an interesting experimental system in which to investigate the mechanisms responsible for maintaining plastid stability.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Production of haploid embryos and plants in Iranian melon (Cucumis melo L.) through irradiated pollen-induced parthenogenesis. Progress of mutant resource development and tilling on starch biosynthesis in wheat. Scandinavian mutation research during the past 90 years - a historical review. Impact of mutant varieties in Malaysia: challenges and future perspectives for mutation breeding. Induced mutagenesis for improvement of bean (Phaseolus vulgaris L.) production in Bulgaria.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1