Pub Date : 2021-01-01DOI: 10.1079/9781789249095.0013
Leila Bagheri, M. Lotfi, Mansour Nori
Abstract The irradiated pollen technique (IPT) is the most successful haploidization technique within Cucurbitaceae. The influence of gamma-ray doses (250, 350, 450 and 550 Gy), genotypes and stage of development of embryos obtained by IPT on the induction of haploid embryos were studied in several Iranian melon cultivars as well as their hybrids with alien cultivars. Female flowers were pollinated using pollen that had been irradiated with gamma rays. Different shapes and stages of embryos were excised 21-25 days after pollination and cultured on E20A medium. Direct culture, liquid culture and integrated culture methods were used; integrated culture and liquid culture methods showed advantages in increasing the efficiency of haploid plant production in melon breeding programmes. Results revealed that 550 Gy of gamma irradiation was successful in inducing parthenogenesis and fruit development, whereas lower irradiation doses were not effective in inducing haploid embryos. The percentages of embryos per seed were the highest in 'Samsoori' (1.2%) and 'Saveh' (1.1%) cultivars. Some of the heart-shaped and cotyledon-shaped embryos developed into haploid plants. In total, 52 parthenogenic melon plantlets were recovered from 274 embryos via IPT. Production of haploid embryos and haploid plants was strongly influenced by gamma-ray dose, embryo stage and genotype. Indirect methods and chromosome counting performed on the root cells of regenerated plants showed that these plants were haploid (n = x = 12).
摘要辐照花粉技术是葫芦科植物单倍体化最成功的技术。研究了γ射线剂量(250、350、450和550 Gy)、IPT胚胎基因型和发育阶段对诱导伊朗甜瓜单倍体胚胎的影响。雌花用经过伽马射线照射的花粉授粉。授粉后21 ~ 25天，取不同形状和阶段的胚，在E20A培养基上培养。采用直接培养法、液体培养法和综合培养法;在甜瓜育种中，综合培养和液体培养在提高单倍体植株生产效率方面具有优势。结果表明，550 Gy辐照能成功诱导孤雌生殖和果实发育，而较低剂量辐照则不能诱导单倍体胚胎。单粒胚率最高的品种是‘Samsoori’(1.2%)和‘Saveh’(1.1%)。一些心形和子叶形的胚胎发育成单倍体植株。通过IPT技术，共从274个胚胎中获得52个孤雌瓜苗。单倍体胚和单倍体植株的产生受γ射线剂量、胚期和基因型的强烈影响。对再生植株的根细胞进行间接方法和染色体计数表明，这些植株为单倍体(n = x = 12)。
{"title":"Production of haploid embryos and plants in Iranian melon (Cucumis melo L.) through irradiated pollen-induced parthenogenesis.","authors":"Leila Bagheri, M. Lotfi, Mansour Nori","doi":"10.1079/9781789249095.0013","DOIUrl":"https://doi.org/10.1079/9781789249095.0013","url":null,"abstract":"Abstract\u0000 The irradiated pollen technique (IPT) is the most successful haploidization technique within Cucurbitaceae. The influence of gamma-ray doses (250, 350, 450 and 550 Gy), genotypes and stage of development of embryos obtained by IPT on the induction of haploid embryos were studied in several Iranian melon cultivars as well as their hybrids with alien cultivars. Female flowers were pollinated using pollen that had been irradiated with gamma rays. Different shapes and stages of embryos were excised 21-25 days after pollination and cultured on E20A medium. Direct culture, liquid culture and integrated culture methods were used; integrated culture and liquid culture methods showed advantages in increasing the efficiency of haploid plant production in melon breeding programmes. Results revealed that 550 Gy of gamma irradiation was successful in inducing parthenogenesis and fruit development, whereas lower irradiation doses were not effective in inducing haploid embryos. The percentages of embryos per seed were the highest in 'Samsoori' (1.2%) and 'Saveh' (1.1%) cultivars. Some of the heart-shaped and cotyledon-shaped embryos developed into haploid plants. In total, 52 parthenogenic melon plantlets were recovered from 274 embryos via IPT. Production of haploid embryos and haploid plants was strongly influenced by gamma-ray dose, embryo stage and genotype. Indirect methods and chromosome counting performed on the root cells of regenerated plants showed that these plants were haploid (n = x = 12).","PeriodicalId":287197,"journal":{"name":"Mutation breeding, genetic diversity and crop adaptation to climate change","volume":"84 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124895061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1079/9781789249095.0030
A. Sevanthi, P. Kale, C. Prakash, M. Ramkumar, Neera Yadav, V. Sureshkumar, Yugandhar Poli, Nitasha Grover, M. Behera, A. Khandual, P. Jeyaparakash, M. Kar, M. Raveendran, S. Manonmani, S. G. Krishnan, S. K. Mangrauthia, N. Sarla, M. Sheshshayee, T. Mohapatra, Ashok K. Singh, N. Singh, Rameshwar Sharma
Abstract The Indian initiative for creating mutant resources in rice has generated 87,000 mutants in the background of a popular drought- and heat-tolerant upland cultivar, Nagina 22 (N22), through EMS mutagenesis. So far, 541 macro-mutants from this resource have been identified, maintained in the mutant garden and characterized in detail based on 44 descriptors pertaining to distinctness, uniformity and stability (DUS) of rice and other agronomic parameters. The similarity index of the mutants was more than 0.6 for nearly 90% of the mutants with respect to DUS descriptors, further establishing the validity of the mutants. The available high-quality sequence resource of N22 has been improved by reducing the gaps by 0.02% in the coding sequence (CDS) region. This was made possible using the newly synthesized whole-genome data of N22 which helped to remove 9006 'Ns' and replace 12,746 existing nucleotides with the accurate ones. These sequence and morphological details have been updated in the mutant database 'EMSgardeN22'. Further, 1058 mutants have been identified for low-P tolerance, tolerance to sheath blight, blast, drought, heat, higher photosynthetic efficiency and agronomic and root traits from this resource. A novel herbicide-tolerant (imazethapyr) mutant earlier identified and characterized from this resource is now being used in introgressing the herbicide-tolerant trait in eight major rice varieties in India. Further, robust and simpler screening systems have been tested for studying low-P tolerance of the mutants. A grain-size mutant, heat-tolerant mutant, drought-tolerant mutant, stay-green mutant and low-P tolerant and water-use efficient high-root-volume mutants have been characterized at morphological and molecular levels. A brief account of all these mutants, the entire mutant resource and the elaborate trait-based screenings is presented in this chapter.
{"title":"National repository of EMS induced mutants of an upland rice cultivar Nagina 22: progress update on characterization and utilization.","authors":"A. Sevanthi, P. Kale, C. Prakash, M. Ramkumar, Neera Yadav, V. Sureshkumar, Yugandhar Poli, Nitasha Grover, M. Behera, A. Khandual, P. Jeyaparakash, M. Kar, M. Raveendran, S. Manonmani, S. G. Krishnan, S. K. Mangrauthia, N. Sarla, M. Sheshshayee, T. Mohapatra, Ashok K. Singh, N. Singh, Rameshwar Sharma","doi":"10.1079/9781789249095.0030","DOIUrl":"https://doi.org/10.1079/9781789249095.0030","url":null,"abstract":"Abstract\u0000 The Indian initiative for creating mutant resources in rice has generated 87,000 mutants in the background of a popular drought- and heat-tolerant upland cultivar, Nagina 22 (N22), through EMS mutagenesis. So far, 541 macro-mutants from this resource have been identified, maintained in the mutant garden and characterized in detail based on 44 descriptors pertaining to distinctness, uniformity and stability (DUS) of rice and other agronomic parameters. The similarity index of the mutants was more than 0.6 for nearly 90% of the mutants with respect to DUS descriptors, further establishing the validity of the mutants. The available high-quality sequence resource of N22 has been improved by reducing the gaps by 0.02% in the coding sequence (CDS) region. This was made possible using the newly synthesized whole-genome data of N22 which helped to remove 9006 'Ns' and replace 12,746 existing nucleotides with the accurate ones. These sequence and morphological details have been updated in the mutant database 'EMSgardeN22'. Further, 1058 mutants have been identified for low-P tolerance, tolerance to sheath blight, blast, drought, heat, higher photosynthetic efficiency and agronomic and root traits from this resource. A novel herbicide-tolerant (imazethapyr) mutant earlier identified and characterized from this resource is now being used in introgressing the herbicide-tolerant trait in eight major rice varieties in India. Further, robust and simpler screening systems have been tested for studying low-P tolerance of the mutants. A grain-size mutant, heat-tolerant mutant, drought-tolerant mutant, stay-green mutant and low-P tolerant and water-use efficient high-root-volume mutants have been characterized at morphological and molecular levels. A brief account of all these mutants, the entire mutant resource and the elaborate trait-based screenings is presented in this chapter.","PeriodicalId":287197,"journal":{"name":"Mutation breeding, genetic diversity and crop adaptation to climate change","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115818421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1079/9781789249095.0025
E. Abdalla, T. Ahmed, Omar Bakhit, Y. Gamar, Salih Elshaikh, Y. Mohammed, Abdellatif Sulaiman And Hatim Mardi
Abstract Groundnut (Arachis hypogaea L.), produced in the traditional small-scale rainfed sector of Western Sudan, accounts for 80% of the total annual groundnut acreage, producing 70% of the total production. Low productivity of groundnut is a characteristic feature in North Kordofan State, which is characterized as the most vulnerable state to the impact of climate change. Terminal drought stress resulting from reduction in rainfall amount and distribution at the end of the season is the most deleterious drought period, as it coincides with groundnut pod filling and maturation periods. High and stable yields under subsistence farming conditions in North Kordofan State could be realized only by using adapted high-yielding, drought-tolerant genotypes. Mutation induction by gamma-rays of 200 and 300 Gy was utilized to irradiate 500 dry seeds of the Spanish-type groundnut genotypes, Barberton, Sodari, ICGV 89104, ICGV 86743, ICGV 86744 and ICG 221, aiming at increasing the chances of obtaining genotypes with the desired drought-tolerant traits. Mutants were selected from the M3 plants using visual morphological traits. Groundnut mutants at the M4 and M5 generations, advanced by single seed descent, were evaluated for end-of-season drought tolerance. A terminal drought period of 25 days was imposed after 60 days from planting, using a rainout shelter. Mutants that survived 25 days of terminal drought stress were further evaluated for agronomic performance under rainfed field conditions. The groundnut mutant, Barberton-b-30-3-B, produced 1024 kg/ha, a significantly higher mean pod yield over 12 seasons compared with 926 kg/ha for 'Gubeish', the widely grown released check cultivar, showing overall yield advantage of 11%. Under 5 years of participatory research, Barberton-b-30-3-B was ranked the best with yield increment of 21% over 'Gubeish' under the mother trials. The GGE biplot analysis for 12 and five seasons, respectively, showed that Barberton-b-30-3-B was stable and produced a good yield in both high and low rainfall situations. Hence, Barberton-b-30-3-B was found to be a suitable mutant for sustainable profitable yields in the marginal dry lands of North Kordofan State and was officially released as 'Tafra-1' by the National Variety Release Committee during its second meeting of April 2018.
{"title":"Groundnut mutants with end-of-season drought tolerance for the marginal dry lands of North Kordofan State, Sudan.","authors":"E. Abdalla, T. Ahmed, Omar Bakhit, Y. Gamar, Salih Elshaikh, Y. Mohammed, Abdellatif Sulaiman And Hatim Mardi","doi":"10.1079/9781789249095.0025","DOIUrl":"https://doi.org/10.1079/9781789249095.0025","url":null,"abstract":"Abstract\u0000 Groundnut (Arachis hypogaea L.), produced in the traditional small-scale rainfed sector of Western Sudan, accounts for 80% of the total annual groundnut acreage, producing 70% of the total production. Low productivity of groundnut is a characteristic feature in North Kordofan State, which is characterized as the most vulnerable state to the impact of climate change. Terminal drought stress resulting from reduction in rainfall amount and distribution at the end of the season is the most deleterious drought period, as it coincides with groundnut pod filling and maturation periods. High and stable yields under subsistence farming conditions in North Kordofan State could be realized only by using adapted high-yielding, drought-tolerant genotypes. Mutation induction by gamma-rays of 200 and 300 Gy was utilized to irradiate 500 dry seeds of the Spanish-type groundnut genotypes, Barberton, Sodari, ICGV 89104, ICGV 86743, ICGV 86744 and ICG 221, aiming at increasing the chances of obtaining genotypes with the desired drought-tolerant traits. Mutants were selected from the M3 plants using visual morphological traits. Groundnut mutants at the M4 and M5 generations, advanced by single seed descent, were evaluated for end-of-season drought tolerance. A terminal drought period of 25 days was imposed after 60 days from planting, using a rainout shelter. Mutants that survived 25 days of terminal drought stress were further evaluated for agronomic performance under rainfed field conditions. The groundnut mutant, Barberton-b-30-3-B, produced 1024 kg/ha, a significantly higher mean pod yield over 12 seasons compared with 926 kg/ha for 'Gubeish', the widely grown released check cultivar, showing overall yield advantage of 11%. Under 5 years of participatory research, Barberton-b-30-3-B was ranked the best with yield increment of 21% over 'Gubeish' under the mother trials. The GGE biplot analysis for 12 and five seasons, respectively, showed that Barberton-b-30-3-B was stable and produced a good yield in both high and low rainfall situations. Hence, Barberton-b-30-3-B was found to be a suitable mutant for sustainable profitable yields in the marginal dry lands of North Kordofan State and was officially released as 'Tafra-1' by the National Variety Release Committee during its second meeting of April 2018.","PeriodicalId":287197,"journal":{"name":"Mutation breeding, genetic diversity and crop adaptation to climate change","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121089435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1079/9781789249095.0020
S. Begum, M. Islam, Rigyan Gupta
Abstract Chickpea has a high yield potential, nutritional importance and diversity of use. A mutation breeding programme was undertaken at Bangladesh Institute of Nuclear Agriculture (BINA) with a view to developing early-maturing, large-seeded and high-yielding varieties of chickpea. Seeds of the popular chickpea variety 'Desi Binasola-2' were treated with different doses of gamma-rays (200, 300 and 400 Gy). The treated seeds were grown in batches according to dose for raising the M1 generation. M2 seeds were collected from individual M1 plants and subsequently grown in plant-progeny rows in the M2 generation and selections were made from the M2 families. Only 85 plants were selected from the M2 population and these were grown in the M3 generation. The mutant 'CPM-kabuli' and 28 other mutants were selected from M3 and were grown in the M4 generation. Only five mutants, including 'CPM-kabuli', were selected from M4 and were grown in M5. The selected mutant 'CPM-kabuli' along with check varieties were put into preliminary yield trials. Finally, the mutant lines were evaluated, with respect to two check varieties, in advanced, zonal-yield, on-farm and on-station trials in successive generations. All the selected mutant lines were grown at different locations in Bangladesh to observe the yield and other characteristics. The performance of the mutants was evaluated under two management practices: research management and farmers' management. Contrary to its parent, 'CPM-kabuli' was found to be tolerant to root rot and Botrytis grey mould, and also showed greater tolerance to pod borer insect-pest infestation than other mutants and check varieties. The main improved attributes are a cream seed coat colour, which reflects kabuli type, larger seed size and higher seed yield. The mutant 'CPM-kabuli' matures in the range of 115-125 days and is high yielding (1.7 t/ha). Considering all these, the bold Kabuli type chickpea mutant 'CPM-kabuli' was registered as the variety 'Binasola-9' for commercial cultivation during 2017 and is suitable for farmers in drought-prone areas in Bangladesh.
{"title":"Development of the first kabuli type chickpea mutant variety in Bangladesh.","authors":"S. Begum, M. Islam, Rigyan Gupta","doi":"10.1079/9781789249095.0020","DOIUrl":"https://doi.org/10.1079/9781789249095.0020","url":null,"abstract":"Abstract\u0000 Chickpea has a high yield potential, nutritional importance and diversity of use. A mutation breeding programme was undertaken at Bangladesh Institute of Nuclear Agriculture (BINA) with a view to developing early-maturing, large-seeded and high-yielding varieties of chickpea. Seeds of the popular chickpea variety 'Desi Binasola-2' were treated with different doses of gamma-rays (200, 300 and 400 Gy). The treated seeds were grown in batches according to dose for raising the M1 generation. M2 seeds were collected from individual M1 plants and subsequently grown in plant-progeny rows in the M2 generation and selections were made from the M2 families. Only 85 plants were selected from the M2 population and these were grown in the M3 generation. The mutant 'CPM-kabuli' and 28 other mutants were selected from M3 and were grown in the M4 generation. Only five mutants, including 'CPM-kabuli', were selected from M4 and were grown in M5. The selected mutant 'CPM-kabuli' along with check varieties were put into preliminary yield trials. Finally, the mutant lines were evaluated, with respect to two check varieties, in advanced, zonal-yield, on-farm and on-station trials in successive generations. All the selected mutant lines were grown at different locations in Bangladesh to observe the yield and other characteristics. The performance of the mutants was evaluated under two management practices: research management and farmers' management. Contrary to its parent, 'CPM-kabuli' was found to be tolerant to root rot and Botrytis grey mould, and also showed greater tolerance to pod borer insect-pest infestation than other mutants and check varieties. The main improved attributes are a cream seed coat colour, which reflects kabuli type, larger seed size and higher seed yield. The mutant 'CPM-kabuli' matures in the range of 115-125 days and is high yielding (1.7 t/ha). Considering all these, the bold Kabuli type chickpea mutant 'CPM-kabuli' was registered as the variety 'Binasola-9' for commercial cultivation during 2017 and is suitable for farmers in drought-prone areas in Bangladesh.","PeriodicalId":287197,"journal":{"name":"Mutation breeding, genetic diversity and crop adaptation to climate change","volume":"64 6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123446681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1079/9781789249095.0042
T. Abe, H. Ichida, Y. Hayashi, R. Morita, Y. Shirakawa, K. Ishii, Tadashi Sato, Hiroki Saito, Y. Okumoto
Abstract We have developed a unique technology for mutation induction of plants using energetic ion beams at the RI Beam Factory (RIBF) of Rikagaku Kenkyūjo (RIKEN) (Institute of Physical and Chemical Research). Ion beams effectively induce mutations at relatively low doses without severely inhibiting growth. The irradiation treatment can be given to various plant materials and mutation can be induced in a short time, between seconds and a few minutes. The linear energy transfer (LET) of ions depends on the nuclide and velocity. Since LET value affects the mutation frequency, it is an important parameter to determine the most effective irradiation condition in mutagenesis. We determined the most effective dose in each LET for mutation induction in imbibed rice seeds. Subsequently, we analysed the mutated DNA responsible for the phenotype in morphological mutants. Most of the mutations were small deletions of less than 100 bp. Irradiations of C-ions and Ne-ions are effective for plant breeding because of the very high mutation rate and sufficient energy to disrupt a single gene. On the other hand, all mutations induced by Ar-ion (290 keV/μm) irradiation were large deletions ranging from 176 bp to approximately 620 kb. The average number of mutations in the target exon regions was 7.3, 8.5 and 4.3 per M3 mutant plant in C-ions, Ne-ions and Ar-ions, respectively. The number of mutations induced by heavy-ion irradiation was relatively small. We could identify six responsible genes for eight mutants induced by C-ion and Ne-ion irradiations and two responsible genes for four mutants induced by Ar-ion irradiation. Three of these were genes not previously described.
{"title":"Ion beam mutagenesis - an innovative and effective method for plant breeding and gene discovery.","authors":"T. Abe, H. Ichida, Y. Hayashi, R. Morita, Y. Shirakawa, K. Ishii, Tadashi Sato, Hiroki Saito, Y. Okumoto","doi":"10.1079/9781789249095.0042","DOIUrl":"https://doi.org/10.1079/9781789249095.0042","url":null,"abstract":"Abstract\u0000 We have developed a unique technology for mutation induction of plants using energetic ion beams at the RI Beam Factory (RIBF) of Rikagaku Kenkyūjo (RIKEN) (Institute of Physical and Chemical Research). Ion beams effectively induce mutations at relatively low doses without severely inhibiting growth. The irradiation treatment can be given to various plant materials and mutation can be induced in a short time, between seconds and a few minutes. The linear energy transfer (LET) of ions depends on the nuclide and velocity. Since LET value affects the mutation frequency, it is an important parameter to determine the most effective irradiation condition in mutagenesis. We determined the most effective dose in each LET for mutation induction in imbibed rice seeds. Subsequently, we analysed the mutated DNA responsible for the phenotype in morphological mutants. Most of the mutations were small deletions of less than 100 bp. Irradiations of C-ions and Ne-ions are effective for plant breeding because of the very high mutation rate and sufficient energy to disrupt a single gene. On the other hand, all mutations induced by Ar-ion (290 keV/μm) irradiation were large deletions ranging from 176 bp to approximately 620 kb. The average number of mutations in the target exon regions was 7.3, 8.5 and 4.3 per M3 mutant plant in C-ions, Ne-ions and Ar-ions, respectively. The number of mutations induced by heavy-ion irradiation was relatively small. We could identify six responsible genes for eight mutants induced by C-ion and Ne-ion irradiations and two responsible genes for four mutants induced by Ar-ion irradiation. Three of these were genes not previously described.","PeriodicalId":287197,"journal":{"name":"Mutation breeding, genetic diversity and crop adaptation to climate change","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128693845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1079/9781789249095.0046
Libin Zhou, Y. Du, Zhuo Feng, Tao Cui, Xia Chen, Shanwei Luo, Yuze Chen, H. Feng, Jin-hu Mu, Lixia Yu, Wen‐jian Li
Abstract Mutation breeding induced by irradiation with highly energetic photons and ion beams is one of the important methods to improve plant varieties, but the mutagenic effects and molecular mechanisms are often not entirely clear. Traditional research is focused on phenotype screening, chromosome aberration tests and genetic variation analysis of specific genes. The whole genome sequencing technique provides a new method to understand and undertake the comprehensive identification of mutations caused by irradiations with different linear energy transfer (LET). In this study, ten Arabidopsis thaliana M3 lines induced by carbon-ion beams (CIB) and ten M3 lines induced by gamma-rays were re-sequenced by using the Illumina HiSeq sequencing platform, and the single base substitutions (SBSs) and small insertions or deletions (indels) were analysed comparatively. It was found that the ratio of SBSs to small indels for M3 lines induced by CIB was 2.57:1, whereas the ratio was 1.78:1 for gamma-rays. The ratios of deletions to insertions for carbon ions and gamma-rays were 4.8:1 and 2.8:1, respectively. The single-base indels were more prevalent than those equal to or greater than 2 bp in both CIB and gamma-ray induced M3 lines. Among the detected SBSs, the ratio of transitions to transversions induced by carbon-ion irradiation was 1.01 and 1.42 for gamma-rays; these values differ greatly from the 2.41 reported for spontaneous substitutions. This study provides novel data on molecular characteristics of CIB and gamma-ray induced mutations at the genome-wide scale. It can also provide valuable clues for explaining the potential mechanism of plant mutation breeding by irradiations with different LETs.
{"title":"Comparative study of mutations induced by carbon-ion beams and gamma-ray irradiations in Arabidopsis thaliana at the genome-wide scale.","authors":"Libin Zhou, Y. Du, Zhuo Feng, Tao Cui, Xia Chen, Shanwei Luo, Yuze Chen, H. Feng, Jin-hu Mu, Lixia Yu, Wen‐jian Li","doi":"10.1079/9781789249095.0046","DOIUrl":"https://doi.org/10.1079/9781789249095.0046","url":null,"abstract":"Abstract\u0000 Mutation breeding induced by irradiation with highly energetic photons and ion beams is one of the important methods to improve plant varieties, but the mutagenic effects and molecular mechanisms are often not entirely clear. Traditional research is focused on phenotype screening, chromosome aberration tests and genetic variation analysis of specific genes. The whole genome sequencing technique provides a new method to understand and undertake the comprehensive identification of mutations caused by irradiations with different linear energy transfer (LET). In this study, ten Arabidopsis thaliana M3 lines induced by carbon-ion beams (CIB) and ten M3 lines induced by gamma-rays were re-sequenced by using the Illumina HiSeq sequencing platform, and the single base substitutions (SBSs) and small insertions or deletions (indels) were analysed comparatively. It was found that the ratio of SBSs to small indels for M3 lines induced by CIB was 2.57:1, whereas the ratio was 1.78:1 for gamma-rays. The ratios of deletions to insertions for carbon ions and gamma-rays were 4.8:1 and 2.8:1, respectively. The single-base indels were more prevalent than those equal to or greater than 2 bp in both CIB and gamma-ray induced M3 lines. Among the detected SBSs, the ratio of transitions to transversions induced by carbon-ion irradiation was 1.01 and 1.42 for gamma-rays; these values differ greatly from the 2.41 reported for spontaneous substitutions. This study provides novel data on molecular characteristics of CIB and gamma-ray induced mutations at the genome-wide scale. It can also provide valuable clues for explaining the potential mechanism of plant mutation breeding by irradiations with different LETs.","PeriodicalId":287197,"journal":{"name":"Mutation breeding, genetic diversity and crop adaptation to climate change","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129309790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1079/9781789249095.0014
Z. Tadele, K. Assefa, S. Chanyalew, A. Bekele, A. Weichert, M. Schnell, Nora Röckel, Negussu Hussein, G. Cannarozzi
Abstract Induced mutation has been playing a significant role in the improvement of diverse crop types. This led to the release of over 3200 crop varieties in over 70 countries. We implemented induced mutation on tef (Eragrostis tef (Zucc.) Trotter), one of the most important cereal crops in the Horn of Africa, especially in Ethiopia, where it is annually cultivated on over 3 million hectares of land, equivalent to 30% of the total area allocated to cereals. Although tef is extensively cultivated in Ethiopia due to its resilience to diverse environmental stresses, the productivity of the crop is very low. The Tef Improvement Project based at the University of Bern in Switzerland employs mutation breeding to tackle major constraints in tef in order to enhance crop productivity. About 12,000 EMS (ethyl methanesulfonate) mutagenized M2 families were generated from four improved tef varieties, namely 'Tsedey', 'Dukem', 'Kora' and 'Dagim'. Screening for major traits of importance helped us to obtain several candidate lines, including semi-dwarf and lodging-tolerant, drought-tolerant and acid-soil-tolerant lines. Among these, the most promising ones were introgressed to locally adapted improved varieties followed by several years of testing at representative locations for traits of interest. As a result, a new variety called 'Tesfa' with a novel and desirable combination of traits was approved for release to the farming community. This shows that the project has been actively involved in all three phases of induced mutation: mutation induction, mutation detection and mutation breeding.
{"title":"Application of mutation breeding to the improvement of the under-studied crop tef (Eragrostis tef (Zucc.) Trotter).","authors":"Z. Tadele, K. Assefa, S. Chanyalew, A. Bekele, A. Weichert, M. Schnell, Nora Röckel, Negussu Hussein, G. Cannarozzi","doi":"10.1079/9781789249095.0014","DOIUrl":"https://doi.org/10.1079/9781789249095.0014","url":null,"abstract":"Abstract\u0000 Induced mutation has been playing a significant role in the improvement of diverse crop types. This led to the release of over 3200 crop varieties in over 70 countries. We implemented induced mutation on tef (Eragrostis tef (Zucc.) Trotter), one of the most important cereal crops in the Horn of Africa, especially in Ethiopia, where it is annually cultivated on over 3 million hectares of land, equivalent to 30% of the total area allocated to cereals. Although tef is extensively cultivated in Ethiopia due to its resilience to diverse environmental stresses, the productivity of the crop is very low. The Tef Improvement Project based at the University of Bern in Switzerland employs mutation breeding to tackle major constraints in tef in order to enhance crop productivity. About 12,000 EMS (ethyl methanesulfonate) mutagenized M2 families were generated from four improved tef varieties, namely 'Tsedey', 'Dukem', 'Kora' and 'Dagim'. Screening for major traits of importance helped us to obtain several candidate lines, including semi-dwarf and lodging-tolerant, drought-tolerant and acid-soil-tolerant lines. Among these, the most promising ones were introgressed to locally adapted improved varieties followed by several years of testing at representative locations for traits of interest. As a result, a new variety called 'Tesfa' with a novel and desirable combination of traits was approved for release to the farming community. This shows that the project has been actively involved in all three phases of induced mutation: mutation induction, mutation detection and mutation breeding.","PeriodicalId":287197,"journal":{"name":"Mutation breeding, genetic diversity and crop adaptation to climate change","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116282082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1079/9781789249095.0031
Souframanien Jegadeesan, K. S. Reddy
Abstract Black gram (Vigna mungo (L.) Hepper), popularly known as urdbean or mash or black gram, is a grain legume rich in protein (25-28%), widely cultivated in the Indian subcontinent and to a lesser extent in Thailand, Australia and other Asian and South Pacific countries. Genetic improvement in this crop is hindered due to the narrow genetic base. As genetic variability is a prerequisite for any crop improvement programme, induced mutations provide an important source for generating variability. Radiation (gamma, X-rays and neutron) induced mutants were identified for various morphological and biochemical traits, creating a pool of genetic variability. These mutants were used in a cross-breeding programme to develop high-yielding, disease-resistant varieties in black gram. The effective blend of mutation and recombination breeding at the Bhabha Atomic Research Centre has resulted in the release of five black gram varieties (TAU-1, TAU-2, TPU-4, TU94-2 and TU-40) by incorporating desirable traits like large seed, wider adaptability, resistance to disease and improved quality. These varieties have been developed from mutants directly or by using them in cross-breeding programmes. For example, a black gram variety, N0.55, was irradiated with gamma-rays and electron beams to obtain a large number of mutants. The large-seed mutants, UM-196 and UM-201, were used in cross-breeding with the elite cultivar T-9 for developing the high-yielding varieties TAU-1, TAU-2, TPU-4, TU94-2 and TU-40. TAU-1 has become the most popular variety in Maharashtra state, occupying the maximum area under black gram cultivation. Induced mutations will continue to play an increasing role in generating genetic variability for various traits as a major component of environmentally sustainable agriculture.
{"title":"Radiation-induced mutations in genetic enhancement and development of new crop varieties in black gram (Vigna mungo (L.) Hepper).","authors":"Souframanien Jegadeesan, K. S. Reddy","doi":"10.1079/9781789249095.0031","DOIUrl":"https://doi.org/10.1079/9781789249095.0031","url":null,"abstract":"Abstract\u0000 Black gram (Vigna mungo (L.) Hepper), popularly known as urdbean or mash or black gram, is a grain legume rich in protein (25-28%), widely cultivated in the Indian subcontinent and to a lesser extent in Thailand, Australia and other Asian and South Pacific countries. Genetic improvement in this crop is hindered due to the narrow genetic base. As genetic variability is a prerequisite for any crop improvement programme, induced mutations provide an important source for generating variability. Radiation (gamma, X-rays and neutron) induced mutants were identified for various morphological and biochemical traits, creating a pool of genetic variability. These mutants were used in a cross-breeding programme to develop high-yielding, disease-resistant varieties in black gram. The effective blend of mutation and recombination breeding at the Bhabha Atomic Research Centre has resulted in the release of five black gram varieties (TAU-1, TAU-2, TPU-4, TU94-2 and TU-40) by incorporating desirable traits like large seed, wider adaptability, resistance to disease and improved quality. These varieties have been developed from mutants directly or by using them in cross-breeding programmes. For example, a black gram variety, N0.55, was irradiated with gamma-rays and electron beams to obtain a large number of mutants. The large-seed mutants, UM-196 and UM-201, were used in cross-breeding with the elite cultivar T-9 for developing the high-yielding varieties TAU-1, TAU-2, TPU-4, TU94-2 and TU-40. TAU-1 has become the most popular variety in Maharashtra state, occupying the maximum area under black gram cultivation. Induced mutations will continue to play an increasing role in generating genetic variability for various traits as a major component of environmentally sustainable agriculture.","PeriodicalId":287197,"journal":{"name":"Mutation breeding, genetic diversity and crop adaptation to climate change","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132020138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1079/9781789249095.0036
S. Bado, F. Maghuly, V. Várzea, M. Laimer
Abstract Coffee is one of the most valuable commodity tree crops worldwide. However, it suffers from several devastating diseases and pests, for example coffee leaf rust and coffee berry borer, whose impact is being amplified by changing climatic conditions. Development of new adapted varieties remains a laborious effort by conventional breeding due to the long juvenile period in tree crops. Plant cell/tissue culture represents the ultimate method to produce large amounts of true-to-type healthy plants and of explants for mutation breeding. In fact, mutation induction combined with in vitro cell/tissue culture techniques has proved to be effective for developing improved cultivars of perennial crops. Prior to mutation breeding, cell and tissue radiosensitivity tests to various mutagens need to be performed, so that optimal treatments can be applied for large population development. Thus, different in vitro explants (plantlet, leaf, callus, embryogenic callus, globular and torpedo stage embryos) of Coffea arabica and Coffea canephora were exposed to different gamma-ray doses (0, 10, 15, 20, 40, 60 and 80 Gy). After 9-21 weeks incubation, a radiosensitivity test was conducted on the different explants and LD50 doses corresponding to 50% of viability or survival of callus, embryogenic callus, globular and torpedo stage embryos and 50% growth reduction (GR50) of shoot were also determined. Callus explants showed a relatively high radio-resistance (LD30-LD50 50-100 Gy) in comparison with entire plantlets or embryos (LD30-GR50 8-46 Gy). Globular embryo development into plantlets and also leaf area of irradiated plantlets were more severely affected by irradiation than other explants. It was possible to confirm the relative radio-resistance of unicellular explants compared with multicellular explants. Estimation of optimal mutation induction dosage range for various in vitro explants is important for tree crops, especially for coffee improvement.
{"title":"Mutagenesis of in vitro explants of Coffea spp. to induce fungal resistance.","authors":"S. Bado, F. Maghuly, V. Várzea, M. Laimer","doi":"10.1079/9781789249095.0036","DOIUrl":"https://doi.org/10.1079/9781789249095.0036","url":null,"abstract":"Abstract\u0000 Coffee is one of the most valuable commodity tree crops worldwide. However, it suffers from several devastating diseases and pests, for example coffee leaf rust and coffee berry borer, whose impact is being amplified by changing climatic conditions. Development of new adapted varieties remains a laborious effort by conventional breeding due to the long juvenile period in tree crops. Plant cell/tissue culture represents the ultimate method to produce large amounts of true-to-type healthy plants and of explants for mutation breeding. In fact, mutation induction combined with in vitro cell/tissue culture techniques has proved to be effective for developing improved cultivars of perennial crops. Prior to mutation breeding, cell and tissue radiosensitivity tests to various mutagens need to be performed, so that optimal treatments can be applied for large population development. Thus, different in vitro explants (plantlet, leaf, callus, embryogenic callus, globular and torpedo stage embryos) of Coffea arabica and Coffea canephora were exposed to different gamma-ray doses (0, 10, 15, 20, 40, 60 and 80 Gy). After 9-21 weeks incubation, a radiosensitivity test was conducted on the different explants and LD50 doses corresponding to 50% of viability or survival of callus, embryogenic callus, globular and torpedo stage embryos and 50% growth reduction (GR50) of shoot were also determined. Callus explants showed a relatively high radio-resistance (LD30-LD50 50-100 Gy) in comparison with entire plantlets or embryos (LD30-GR50 8-46 Gy). Globular embryo development into plantlets and also leaf area of irradiated plantlets were more severely affected by irradiation than other explants. It was possible to confirm the relative radio-resistance of unicellular explants compared with multicellular explants. Estimation of optimal mutation induction dosage range for various in vitro explants is important for tree crops, especially for coffee improvement.","PeriodicalId":287197,"journal":{"name":"Mutation breeding, genetic diversity and crop adaptation to climate change","volume":"264 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130051009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1900-01-01DOI: 10.1079/9781789249095.0038
J. Jankowicz-Cieslak, F. Goessnitzer, S. Datta, A. Viljoen, I. Ingelbrecht, B. Till
Abstract Bananas are a staple for more than 400 million people. Additionally, more than 16.5 million tonnes are exported, making it both an important food security and a cash crop. Productivity of Cavendish-type bananas is threatened by both abiotic and biotic stresses. The fact that triploid bananas are sterile, parthenocarpic and obligate vegetatively propagated makes them particularly susceptible to diseases, including Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (Foc) tropical race 4 (Foc TR4). This is because continual clonal propagation has led to loss of genetic diversity. Additionally, lack of meiosis limits methods for breeding. Foc TR4 has been devastating Cavendish bananas in South-east Asia but has recently also been reported from Queensland in Australia, the Middle East and Mozambique, thus threatening global banana production. To address this, we are performing mutagenesis of in vitro propagated bananas to broaden the genetic diversity in order to find new alleles conferring disease resistance. We have developed methods for efficient induction of mutations in isolated apical meristems from shoot tips using chemical mutagens and ionizing radiation. Mutation discovery methods have been adapted to recover mutations including single point mutations and large deletions spanning millions of base pairs. We have created approximately 5000 mutated lines for forward-genetic screens to identify TR4 resistance in greenhouse- evaluated material. A subset of ca. 500 in vitro plantlets was subjected to glasshouse-based screening using a virulent F. oxysporum isolate. To date, 23 lines showing altered resistance responses to Foc TR4 have been identified.
香蕉是4亿多人的主食。此外，出口超过1650万吨，使其成为一种重要的粮食安全和经济作物。卡文迪什型香蕉的产量受到非生物和生物胁迫的威胁。事实上，三倍体香蕉是不育的、单性生殖的和专性无性繁殖的，这使得它们特别容易受到疾病的影响，包括由Fusarium oxysporum f. sp. cubense (Foc)热带小种4 (Foc TR4)引起的枯萎病。这是因为连续的无性繁殖导致了遗传多样性的丧失。此外，减数分裂的缺乏限制了育种方法。Foc TR4对东南亚的卡文迪什香蕉造成了破坏，但最近澳大利亚昆士兰、中东和莫桑比克也报告了这一情况，从而威胁到全球香蕉生产。为了解决这个问题，我们正在对体外繁殖的香蕉进行诱变，以扩大遗传多样性，从而找到新的抗病等位基因。我们已经开发了利用化学诱变剂和电离辐射有效诱导离体茎尖分生组织突变的方法。突变发现方法已经适应于恢复突变，包括单点突变和跨越数百万碱基对的大缺失。我们已经创建了大约5000个突变系用于前向遗传筛选，以鉴定温室评估材料的TR4抗性。约500个试管苗的一个亚群使用毒力强的尖孢镰刀菌分离物进行了基于温室的筛选。迄今为止，已确定23个菌株对Foc TR4表现出改变的耐药反应。
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