{"title":"[培养L-929细胞的生长期对其对霍乱弧菌溶细胞素作用敏感性的影响]。","authors":"Iu L Kiselev, A O Tsittser","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The activity of purified V. cholerae cytolysin was estimated from its cytotoxic effect on L-929 cells in various growth phases. The period of target cell development was supposed to influence the sensitivity of the test. Cytolysin preparation is thermolabile, and its effect is neutralized with homologous antiserum.</p>","PeriodicalId":18012,"journal":{"name":"Laboratornoe delo","volume":" 5","pages":"59-60"},"PeriodicalIF":0.0000,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[The effect of the growth period of a culture of L-929 cells on its sensitivity to the action of Vibrio cholerae cytolysin].\",\"authors\":\"Iu L Kiselev, A O Tsittser\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The activity of purified V. cholerae cytolysin was estimated from its cytotoxic effect on L-929 cells in various growth phases. The period of target cell development was supposed to influence the sensitivity of the test. Cytolysin preparation is thermolabile, and its effect is neutralized with homologous antiserum.</p>\",\"PeriodicalId\":18012,\"journal\":{\"name\":\"Laboratornoe delo\",\"volume\":\" 5\",\"pages\":\"59-60\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1991-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Laboratornoe delo\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Laboratornoe delo","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
[The effect of the growth period of a culture of L-929 cells on its sensitivity to the action of Vibrio cholerae cytolysin].
The activity of purified V. cholerae cytolysin was estimated from its cytotoxic effect on L-929 cells in various growth phases. The period of target cell development was supposed to influence the sensitivity of the test. Cytolysin preparation is thermolabile, and its effect is neutralized with homologous antiserum.
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