紫毛狼尾草提取物对芒果炭疽病病原菌炭疽病菌的抑制潜力

T. Chuah, Eh Teet Sudau
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摘要

炭疽病是由芒果炭疽菌引起的芒果主要采前和采后病害。采用体外和体内实验研究了紫荆草提取物对黄孢霉的抑菌活性。结果:体外实验结果表明,紫荆甲醇粗提物对黄颡鱼蛾的抑菌活性最佳,平均最低抑菌浓度为3.13 mg/ml,总抑菌活性最高,为5.28 ml/g。同时,体内实验结果表明,与其他处理相比,褐藻酸盐粗提物包被浓度为18 mg/ml的果实最有效地保持了果实的硬度,且损伤直径最小(0.34 cm)。甲醇粗提物的植物化学分析结果显示,含酚类化合物(单宁)、生物碱和黄酮类化合物,但不含类固醇、皂苷、氨基酸和油。结论:褐藻酸盐基包衣与紫荆枝甲醇粗提物结合可有效抑制芒果炭疽病病原菌,从而延长芒果果实的保质期。关键词:炭疽病,包衣,复方,植物提取物,芒果
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Potential of Pennisetum purpureum weed extract on inhibition of anthracnose pathogen, Colletotrichum gloeosporioides on mango fruits
Anthracnose is the major pre- and postharvest disease of mango which caused by Colletotrichum gloeosporioides. In-vitro and in-vivo studies were done to evaluate the antifungal activity of Pennisetum purpureum weed extract on control of C. gloeosporioides. Result: The in-vitro result showed that methanol crude extract of P. purpureum exhibited the best antifungal activity against C. gloeosprioides with an average minimum inhibition concentration value of 3.13 mg/ml and it had the highest total activity (5.28 ml/g) on inhibition of C. gloeosprioides. Meanwhile, the in-vivo result demonstrated that the fruits coated with alginate incorporated crude extract at a concentration of 18 mg/ml were most effective in retaining fruit firmness and had the smallest size lesion in diameter (0.34 cm) as compared to other treatments. The phytochemical assay of methanolic crude extract revealed the presence of phenolic compound (tannins), alkaloids and flavonoids but steroids, saponin, amino acid and oil were absent. Conclusion: This study has demonstrated that alginate based coating incorporated with methanolic crude extract of P. purpureum could be used to inhibit anthracnose pathogen of C. gloeosporioides, thereby extending the shelf life of mango fruits. Keywords: anthracnose, coating, compound, plant extract, mango
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