{"title":"三种不同类型的石斛兰使用四种媒体配方","authors":"Elizabeth Handini, Dwi Murti Puspitaningtyas","doi":"10.14203/BKR.V23I3.647","DOIUrl":null,"url":null,"abstract":"Dendrobium is an important genus in Orchidaceae because it is widely used in crossbreeding and cut flowers. Seed is a valuable material for propagation as well as useful for maintaining genetic diversity. Seed banks play an important role in orchid conservation. This study aimed to find out the viability of Dendrobium macrophyllum, D. discolor, and D. crumenatum seeds, after being stored for 10 years, predicting the lifespan of seed, and knowing the appropriate germination medium. The method of testing seed viability is directly by germinating the seeds orchids on 4 culture media and calculate the percentage of seed germination. Seeds are stored at -20 ° C. Seed viability test was carried out in media: that are modified of Knudson'C (KC), Vacin and Went (VWs), Hyponex (HS) and Knudson C + micro elements (KCA). The results show that D. discolor seeds can be stored for up to 60 years, D. macrophyllum seeds can last up to 20 years, while D. crumenatum seeds lose viability after 9 years of storage. After nine years, the highest percentage of germination achieved by D. discolor was around 52.17%. The best medium for D. macrophyllum and D. crumenatum seed germination was KC. While D. discolor seeds germinated well on HS media.","PeriodicalId":274763,"journal":{"name":"Buletin Kebun Raya","volume":"30 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2020-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"PERKECAMBAHAN DAN PREDIKSI MASA HIDUP TIGA JENIS Dendrobium MENGGUNAKAN EMPAT MACAM FORMULASI MEDIA\",\"authors\":\"Elizabeth Handini, Dwi Murti Puspitaningtyas\",\"doi\":\"10.14203/BKR.V23I3.647\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Dendrobium is an important genus in Orchidaceae because it is widely used in crossbreeding and cut flowers. Seed is a valuable material for propagation as well as useful for maintaining genetic diversity. Seed banks play an important role in orchid conservation. This study aimed to find out the viability of Dendrobium macrophyllum, D. discolor, and D. crumenatum seeds, after being stored for 10 years, predicting the lifespan of seed, and knowing the appropriate germination medium. The method of testing seed viability is directly by germinating the seeds orchids on 4 culture media and calculate the percentage of seed germination. Seeds are stored at -20 ° C. Seed viability test was carried out in media: that are modified of Knudson'C (KC), Vacin and Went (VWs), Hyponex (HS) and Knudson C + micro elements (KCA). The results show that D. discolor seeds can be stored for up to 60 years, D. macrophyllum seeds can last up to 20 years, while D. crumenatum seeds lose viability after 9 years of storage. After nine years, the highest percentage of germination achieved by D. discolor was around 52.17%. The best medium for D. macrophyllum and D. crumenatum seed germination was KC. While D. discolor seeds germinated well on HS media.\",\"PeriodicalId\":274763,\"journal\":{\"name\":\"Buletin Kebun Raya\",\"volume\":\"30 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-11-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Buletin Kebun Raya\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.14203/BKR.V23I3.647\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Buletin Kebun Raya","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.14203/BKR.V23I3.647","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
石斛是兰科植物中重要的属,广泛用于杂交育种和切花。种子是一种有价值的繁殖材料,也是保持遗传多样性的有用材料。种子库在兰花保护中发挥着重要作用。本研究旨在研究巨叶石斛、变色石斛和皱状石斛种子在贮存10年后的生存能力,预测种子寿命,确定合适的萌发培养基。测定种子活力的方法是直接将兰花种子在4种培养基上萌发,计算种子发芽率。种子在-20℃下保存,在Knudson’C (KC)、Vacin and go (VWs)、Hyponex (HS)和Knudson C +微量元素(KCA)改性的培养基中进行种子活力试验。结果表明,黄花龙葵种子可保存60年,大叶龙葵种子可保存20年,而黄花龙葵种子在保存9年后失去活力。经过9年处理,褐变菊发芽率最高,约为52.17%。其中,KC培养基对大叶金莲种子萌发效果最好,HS培养基对大叶金莲种子萌发效果最好。
PERKECAMBAHAN DAN PREDIKSI MASA HIDUP TIGA JENIS Dendrobium MENGGUNAKAN EMPAT MACAM FORMULASI MEDIA
Dendrobium is an important genus in Orchidaceae because it is widely used in crossbreeding and cut flowers. Seed is a valuable material for propagation as well as useful for maintaining genetic diversity. Seed banks play an important role in orchid conservation. This study aimed to find out the viability of Dendrobium macrophyllum, D. discolor, and D. crumenatum seeds, after being stored for 10 years, predicting the lifespan of seed, and knowing the appropriate germination medium. The method of testing seed viability is directly by germinating the seeds orchids on 4 culture media and calculate the percentage of seed germination. Seeds are stored at -20 ° C. Seed viability test was carried out in media: that are modified of Knudson'C (KC), Vacin and Went (VWs), Hyponex (HS) and Knudson C + micro elements (KCA). The results show that D. discolor seeds can be stored for up to 60 years, D. macrophyllum seeds can last up to 20 years, while D. crumenatum seeds lose viability after 9 years of storage. After nine years, the highest percentage of germination achieved by D. discolor was around 52.17%. The best medium for D. macrophyllum and D. crumenatum seed germination was KC. While D. discolor seeds germinated well on HS media.