Rapid Biogenic and Uv-Vis Spectroscopic Study of Silver Nanoparticle Synthesis by Methanolic Extract of Piper Species

Nanotechnology is one of the most developing areas which involve utilization of materials with structural dimension in of 1-100 nm. This technology opened a pathway for the synthesis of silver nanoparticle by various physical and chemical methods. The biosynthesis method from plant extracts are considered as alternative as it is relatively simple, nontoxic, and environmentally friendly methods. The objective was to synthesize and characterize silver nanoparticle via ultraviolet visible spectroscopy of silver nanoparticles from three piper species which are Piper nigrum, Piper sarmentosum and Piper betel. Silver nanoparticles were prepared by the reaction of 1 mM of 90 ml silver nitrate and 10 ml methanolic leaf extract of each type of plant that act as reductant and stabilizer. The synthesized nanoparticle was characterized by color changes from green into dark brown with increasing intensity of the color from 10 minutes until 48 hour indicating the completion of silver nanoparticle formation at 48 hour. A UV–Vis spectrum of the methanolic medium containing silver nanoparticles demonstrated a peak at 413 nm, 417 nm and 420 nm for Piper nigrum, Piper sarmentosum and Piper betel respectively that corresponds to the plasmon absorbance of silver nanoparticles. The maximum percentage increase in the rate of absorbance was found specifically at 24 hour in all the three leaf. In conclusion piper species is a source for synthesizing silver nanoparticles and Piper nigrum being an excellent source for the synthesis due to the narrow, sharp peak and showing maximum percentage increase at 24 hour among all the three plants. This study exhibited the potential of three methanolic extract of piper species for the green synthesis of silver nanoparticles.