移动场毛细管电泳中塞分散补偿技术的应用

F. Tatar, Lujun Zhang, J. Bastemeijer, P. Turmezei, J. Mollinger, A. Bossche
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引用次数: 2

摘要

过去对毛细管电泳中的移动场进行了介绍。然而,这些尝试都是不成功的,因为已知电场区域内的流体柱引入了渗透流动剖面的抛物线畸变,从而引入了严重的堵塞分散。本文介绍了一种利用移动场毛细管电泳分离效率的方法,该方法消除了严重的堵塞分散(99%的样品平坦剖面)的缺点。此外,电压保持在低值上,样品的速度降低,分离效率提高。
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Technique for Plug Dispersion Compensation In Moving Field Capillary Electrophoresis Application
In the past times moving field in capillary electrophoresis (CE) have been introduced. However these attempts are unsuccessfully because the fluid column in the known electric field area introduces parabolic distortion of the osmotic flow profile, and in this way it introduces sever plug dispersions. This paper introduces a method to utilize the separation efficiency of moving field capillary electrophoresis, without the drawback of severe plug dispersion (99% sample flat profile). Also the voltage is maintained on the low values and the speed of the sample is reduced and the efficiency of the separation is increased.
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