{"title":"农杆菌介导的ARGOS-LIKE转基因苋菜上胚轴的转化","authors":"R. Taipova, B. R. Kuluev","doi":"10.28983/plamic2020.244","DOIUrl":null,"url":null,"abstract":"The present study describes the results of our research in Agrobacterium-mediated transformation of epicotyl segments of Amaranthus cruentus variety “Bagryanyi” by the ARGOS-LIKE transgene of Arabidopsis thaliana controlled by the 35S promoter. For shoot regeneration from epicotyl segments after Agrobacterium-mediated transformation, Murashige-Skoog (MS) medium containing 13 μM 6-benzylaminopurine and 1 μM α-naphthylacetic acid was used. For the selection of transgenic shoots, 10 mg/L of hygromycin B was added to the MS medium.","PeriodicalId":114265,"journal":{"name":"Abstract book of the 2nd International Scientific Conference \"Plants and Microbes: the Future of Biotechnology\" PLAMIC2020","volume":"7 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Agrobacterium-mediated transformation of Amaranthus cruentus L. epicotyls by the ARGOS-LIKE transgene\",\"authors\":\"R. Taipova, B. R. Kuluev\",\"doi\":\"10.28983/plamic2020.244\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The present study describes the results of our research in Agrobacterium-mediated transformation of epicotyl segments of Amaranthus cruentus variety “Bagryanyi” by the ARGOS-LIKE transgene of Arabidopsis thaliana controlled by the 35S promoter. For shoot regeneration from epicotyl segments after Agrobacterium-mediated transformation, Murashige-Skoog (MS) medium containing 13 μM 6-benzylaminopurine and 1 μM α-naphthylacetic acid was used. For the selection of transgenic shoots, 10 mg/L of hygromycin B was added to the MS medium.\",\"PeriodicalId\":114265,\"journal\":{\"name\":\"Abstract book of the 2nd International Scientific Conference \\\"Plants and Microbes: the Future of Biotechnology\\\" PLAMIC2020\",\"volume\":\"7 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-12-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Abstract book of the 2nd International Scientific Conference \\\"Plants and Microbes: the Future of Biotechnology\\\" PLAMIC2020\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.28983/plamic2020.244\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Abstract book of the 2nd International Scientific Conference \"Plants and Microbes: the Future of Biotechnology\" PLAMIC2020","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.28983/plamic2020.244","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Agrobacterium-mediated transformation of Amaranthus cruentus L. epicotyls by the ARGOS-LIKE transgene
The present study describes the results of our research in Agrobacterium-mediated transformation of epicotyl segments of Amaranthus cruentus variety “Bagryanyi” by the ARGOS-LIKE transgene of Arabidopsis thaliana controlled by the 35S promoter. For shoot regeneration from epicotyl segments after Agrobacterium-mediated transformation, Murashige-Skoog (MS) medium containing 13 μM 6-benzylaminopurine and 1 μM α-naphthylacetic acid was used. For the selection of transgenic shoots, 10 mg/L of hygromycin B was added to the MS medium.
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