The study is aimed at developing methods for creating plants rich in anthocyanins using the CRISPR/Cas9 genome editing technology to improve consumer and agricultural properties.
{"title":"Stimulation of anthocyanin pigmentation in Solanum lycopersicum and Nicotiana tabacum using \u0000CRISPR/Cas9 technology","authors":"A. Sukhareva, E. Mikhaylova, B. Kuluev","doi":"10.28983/plamic2020.237","DOIUrl":"https://doi.org/10.28983/plamic2020.237","url":null,"abstract":"The study is aimed at developing methods for creating plants rich in anthocyanins using the CRISPR/Cas9 genome editing technology to improve consumer and agricultural properties.","PeriodicalId":114265,"journal":{"name":"Abstract book of the 2nd International Scientific Conference \"Plants and Microbes: the Future of Biotechnology\" PLAMIC2020","volume":"94 5","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120974952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The interaction of composite tomato plants carrying constructs with pea genes encoding receptors to Nod factors, with nitrogen-fixing bacteria rhizobia was investigated.
研究了携带豌豆Nod因子受体基因构建体的番茄复合植株与固氮细菌根瘤菌的相互作用。
{"title":"Production and analysis of tomato Solanum lycopersicum composite plants carrying the genes of pea Pisum sativum receptors to rhizobial signaling molecules","authors":"E. Rudaya, E. Dolgikh","doi":"10.28983/plamic2020.208","DOIUrl":"https://doi.org/10.28983/plamic2020.208","url":null,"abstract":"The interaction of composite tomato plants carrying constructs with pea genes encoding receptors to Nod factors, with nitrogen-fixing bacteria rhizobia was investigated.","PeriodicalId":114265,"journal":{"name":"Abstract book of the 2nd International Scientific Conference \"Plants and Microbes: the Future of Biotechnology\" PLAMIC2020","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127104818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Yurkov, A. Kryukov, A. Gorbunova, Y. Mikhaylova, K. Dobryakova, A. Afonin, S. Kurbaniyazov, Alexander V. Rodionov, M. Shishova
The expressions of phosphate transport and carbohydrate metabolism genes in Medicago lupulina with and without Rhizophagus irregularis inoculation were evaluated. Differences in their transcript levels were found under conditions of different phosphorus levels in substrate.
{"title":"Effective arbuscular mycorrhiza development between Medicago lupulina and Rhizophagus irregularis under conditions of different phosphorus levels in the substrate","authors":"A. Yurkov, A. Kryukov, A. Gorbunova, Y. Mikhaylova, K. Dobryakova, A. Afonin, S. Kurbaniyazov, Alexander V. Rodionov, M. Shishova","doi":"10.28983/plamic2020.284","DOIUrl":"https://doi.org/10.28983/plamic2020.284","url":null,"abstract":"The expressions of phosphate transport and carbohydrate metabolism genes in Medicago lupulina with and without Rhizophagus irregularis inoculation were evaluated. Differences in their transcript levels were found under conditions of different phosphorus levels in substrate.","PeriodicalId":114265,"journal":{"name":"Abstract book of the 2nd International Scientific Conference \"Plants and Microbes: the Future of Biotechnology\" PLAMIC2020","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128246853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. V. Safronava, Z. Aleschenkova, I. N. Ananyeva, K. I. Evenkova-Chernetsova
The application of microbial preparations Gordebac, AgroMyc and Baktopin in spring rape culture stimulates the development of nitrogen-fixing and phosphate-mobilizing microbiocenoses in crop rhizosphere.
{"title":"Rape rhizospheric nitrogen-fixing and phosphate-mobilizing biocenoses promoted by microbial preparations","authors":"H. V. Safronava, Z. Aleschenkova, I. N. Ananyeva, K. I. Evenkova-Chernetsova","doi":"10.28983/plamic2020.213","DOIUrl":"https://doi.org/10.28983/plamic2020.213","url":null,"abstract":"The application of microbial preparations Gordebac, AgroMyc and Baktopin in spring rape culture stimulates the development of nitrogen-fixing and phosphate-mobilizing microbiocenoses in crop rhizosphere.","PeriodicalId":114265,"journal":{"name":"Abstract book of the 2nd International Scientific Conference \"Plants and Microbes: the Future of Biotechnology\" PLAMIC2020","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128378665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Shaposhnikov, N. Vishnevskaya, V. Shakhnazarova, D. Syrova, E. Borodina, O. Kovaleva, O. Strunnikova
Inoculation of barley with a F. culmorum not accompanied by an increase in the number of abscisic acid in plants, but under P. fluorescens 2137 inoculation, the accumulation of abscisic acid in the roots occurred.
接种黄芽孢杆菌后，大麦植株脱落酸的数量没有增加，但接种荧光孢杆菌2137后，脱落酸在根系中积累。
{"title":"The effect of Fusarium culmorum and Pseudomonas fluorescens 2137 on the content of abscisic acid in the roots and shoots of barley seedlings","authors":"A. Shaposhnikov, N. Vishnevskaya, V. Shakhnazarova, D. Syrova, E. Borodina, O. Kovaleva, O. Strunnikova","doi":"10.28983/plamic2020.220","DOIUrl":"https://doi.org/10.28983/plamic2020.220","url":null,"abstract":"Inoculation of barley with a F. culmorum not accompanied by an increase in the number of abscisic acid in plants, but under P. fluorescens 2137 inoculation, the accumulation of abscisic acid in the roots occurred.","PeriodicalId":114265,"journal":{"name":"Abstract book of the 2nd International Scientific Conference \"Plants and Microbes: the Future of Biotechnology\" PLAMIC2020","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130950810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The interaction of legumes with rhizobia leads to formation of the symbiotic nodules on their roots, which are specialized plant organs for nitrogen fixation. Considerable progress has been made in deciphering the molecular-genetic and cellular mechanisms of symbiotic nodule development in recent years. However, some aspects of nodule development clearly merit much more attention.
{"title":"Symbiotic nodule development","authors":"V. E. Tsyganov","doi":"10.28983/plamic2020.257","DOIUrl":"https://doi.org/10.28983/plamic2020.257","url":null,"abstract":"The interaction of legumes with rhizobia leads to formation of the symbiotic nodules on their roots, which are specialized plant organs for nitrogen fixation. Considerable progress has been made in deciphering the molecular-genetic and cellular mechanisms of symbiotic nodule development in recent years. However, some aspects of nodule development clearly merit much more attention.","PeriodicalId":114265,"journal":{"name":"Abstract book of the 2nd International Scientific Conference \"Plants and Microbes: the Future of Biotechnology\" PLAMIC2020","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125771651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Low gene expression of CHI and CHS and high expression of PAL and STS were detected in varieties with a high content of stilbenes. A feature of varieties with low stilbene production was a weak level of PAL gene expression.
{"title":"Stilbene biosynthesis in callus culture of grapes of different resistance to pathogens","authors":"M. Sundyreva, E. O. Lutsky, A. Mishko","doi":"10.28983/plamic2020.239","DOIUrl":"https://doi.org/10.28983/plamic2020.239","url":null,"abstract":"Low gene expression of CHI and CHS and high expression of PAL and STS were detected in varieties with a high content of stilbenes. A feature of varieties with low stilbene production was a weak level of PAL gene expression.","PeriodicalId":114265,"journal":{"name":"Abstract book of the 2nd International Scientific Conference \"Plants and Microbes: the Future of Biotechnology\" PLAMIC2020","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130036635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. S. Velichko, A. R. Bagavova, E. Sigida, G. Burygin, Y. P. Fedonenko
Diazotrophic endobionts Herbaspirillum spp. were studied in respect to the structural peculiarities of the lipopolysaccharides (LPS), O-specific polysaccharides (OPS) structure, antigenic composition and genetics.
{"title":"Structural peculiarities of biopolymers produced by diazotrophic endobiont Herbaspirillum spp.","authors":"N. S. Velichko, A. R. Bagavova, E. Sigida, G. Burygin, Y. P. Fedonenko","doi":"10.28983/plamic2020.266","DOIUrl":"https://doi.org/10.28983/plamic2020.266","url":null,"abstract":"Diazotrophic endobionts Herbaspirillum spp. were studied in respect to the structural peculiarities of the lipopolysaccharides (LPS), O-specific polysaccharides (OPS) structure, antigenic composition and genetics.","PeriodicalId":114265,"journal":{"name":"Abstract book of the 2nd International Scientific Conference \"Plants and Microbes: the Future of Biotechnology\" PLAMIC2020","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122375004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The principle of obtaining recombinant antimicrobial polypeptides from plant and microbial origins as a part of chimeric proteins with thioredoxin by heterologous expression in a prokaryotic system is presented. The results obtained in terms of their antifungal activity in relation to plant pathogenic micromycetes allow us to consider these compounds as prototypes of some active substances of environmentally friendly biofungicides, as well as possible components of hybrid plant protection products against fungal diseases.
{"title":"Biotechnology for production of recombinant hybrid proteins from plants and microbes with antifungal activity","authors":"E. Rogozin","doi":"10.28983/plamic2020.206","DOIUrl":"https://doi.org/10.28983/plamic2020.206","url":null,"abstract":"The principle of obtaining recombinant antimicrobial polypeptides from plant and microbial origins as a part of chimeric proteins with thioredoxin by heterologous expression in a prokaryotic system is presented. The results obtained in terms of their antifungal activity in relation to plant pathogenic micromycetes allow us to consider these compounds as prototypes of some active substances of environmentally friendly biofungicides, as well as possible components of hybrid plant protection products against fungal diseases.","PeriodicalId":114265,"journal":{"name":"Abstract book of the 2nd International Scientific Conference \"Plants and Microbes: the Future of Biotechnology\" PLAMIC2020","volume":"79 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132961817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. G. Shpakovski, E. Shematorova, O. Babak, I. Slovokhotov, S. G. Spivak, M. Khaliluev, Y. Doludin, V. N. Klykov, E. Baranova, T. A. Tereshonkova, A. Kilchevsky, G. V. Shpakovski
In plants, along with the brassinosteroid, the older progesterone hormone system also functions. We have characterized some components of this system and shown its importance for enhancing plant immunity.
{"title":"Specific cytochromes P450 and adrenodoxin-like mitochondrial ferredoxins as components of the progesterone hormone system of higher plants involved in comprehensive protection from biotic and abiotic stresse","authors":"D. G. Shpakovski, E. Shematorova, O. Babak, I. Slovokhotov, S. G. Spivak, M. Khaliluev, Y. Doludin, V. N. Klykov, E. Baranova, T. A. Tereshonkova, A. Kilchevsky, G. V. Shpakovski","doi":"10.28983/plamic2020.225","DOIUrl":"https://doi.org/10.28983/plamic2020.225","url":null,"abstract":"In plants, along with the brassinosteroid, the older progesterone hormone system also functions. We have characterized some components of this system and shown its importance for enhancing plant immunity.","PeriodicalId":114265,"journal":{"name":"Abstract book of the 2nd International Scientific Conference \"Plants and Microbes: the Future of Biotechnology\" PLAMIC2020","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131356124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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