2SCA-02 Visualizing stator-protein distributions of bacterial flagellar motors(2SCA Japan-China-Taiwan joint sympoium on cooperativity in supramolcular machine,Symposium,The 52nd Annual Meeting of the Biophysical Society of Japan(BSJ2014))

The bacterial flagellar motor is a natural electrical rotary molecular machine. The stator-unit consists of MotA/B proteins couples ion-flux to motor rotations. More than 10 stator-units in a motor turn over dynamically in response to the cellular energetical conditions and external loads. However, spatial features and temporal dynamics of stator-units have never been studied thoroughly. We have built a super-resolution fluorescent microscope with 10 nm resolution to study stator-units dynamics using dual color labeling on rotorand statorproteins. We will present protein counting, spatial distribution and dynamical properties of stator proteins. We thank Seiji Kojima, Hajime Fukuoka, Akihiko Ishijima and Michio Homma for the bacterial strains.