{"title":"利用RAPD技术估计小麦种质与谷物包囊线虫的亲缘关系","authors":"Y. I. Erum, F. Shahina","doi":"10.18681/PJN.V34.I02.P193","DOIUrl":null,"url":null,"abstract":"Investigations were conducted at molecular level, to estimate genetic diversity among wheat germplasm (20 cultivars/lines) in relation to their response against cereal cyst nematodes Heterodera avenae, through Random Amplified Polymorphic DNA (RAPD) technique. A total of 589 bands were generated using 14 primers with an average of 28.8 bands/genotype. Maximum percentage of polymorphic loci was 92.86% for genotype TJ-83. Inferences have been made regarding bioassay and molecular characterization that the most diverse and resistant genotype against CCN was Moomal-2002 as compared to the rest of the genotypes studied and the most effective loci to screen diversity was OPA-09.","PeriodicalId":207209,"journal":{"name":"Pakistan Journal of Nematology","volume":"13 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2016-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Estimation of genetic relationship of wheat germplasm against cereal cyst nematodes using RAPD technique\",\"authors\":\"Y. I. Erum, F. Shahina\",\"doi\":\"10.18681/PJN.V34.I02.P193\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Investigations were conducted at molecular level, to estimate genetic diversity among wheat germplasm (20 cultivars/lines) in relation to their response against cereal cyst nematodes Heterodera avenae, through Random Amplified Polymorphic DNA (RAPD) technique. A total of 589 bands were generated using 14 primers with an average of 28.8 bands/genotype. Maximum percentage of polymorphic loci was 92.86% for genotype TJ-83. Inferences have been made regarding bioassay and molecular characterization that the most diverse and resistant genotype against CCN was Moomal-2002 as compared to the rest of the genotypes studied and the most effective loci to screen diversity was OPA-09.\",\"PeriodicalId\":207209,\"journal\":{\"name\":\"Pakistan Journal of Nematology\",\"volume\":\"13 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2016-07-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pakistan Journal of Nematology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.18681/PJN.V34.I02.P193\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pakistan Journal of Nematology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18681/PJN.V34.I02.P193","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Estimation of genetic relationship of wheat germplasm against cereal cyst nematodes using RAPD technique
Investigations were conducted at molecular level, to estimate genetic diversity among wheat germplasm (20 cultivars/lines) in relation to their response against cereal cyst nematodes Heterodera avenae, through Random Amplified Polymorphic DNA (RAPD) technique. A total of 589 bands were generated using 14 primers with an average of 28.8 bands/genotype. Maximum percentage of polymorphic loci was 92.86% for genotype TJ-83. Inferences have been made regarding bioassay and molecular characterization that the most diverse and resistant genotype against CCN was Moomal-2002 as compared to the rest of the genotypes studied and the most effective loci to screen diversity was OPA-09.
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