Pub Date : 2021-07-08DOI: 10.17582/journal.pjn/2021.39.1.65.67
Sagir Hussain, Q. Abbas, A. Razzaq, S. Khan
| Cyst nematode, the Heterodera Schmidt (1871) is one of the most widely distributed and economically important plant parasitic nematode genus. During the present investigation of plant-parasitic nematode fauna from Gilgit-Baltistan, Pakistan, four cyst nematode species viz., Heterodera avenae Wollenweber, 1924; H. mani Mathews,1971; H. schachtii Schmidt, 1871 and H. zeae Koshy, Swarup and Sethi, 1971 were detected from all four districts.
{"title":"Distribution of Cyst Nematodes in Gilgit-Baltistan, Pakistan","authors":"Sagir Hussain, Q. Abbas, A. Razzaq, S. Khan","doi":"10.17582/journal.pjn/2021.39.1.65.67","DOIUrl":"https://doi.org/10.17582/journal.pjn/2021.39.1.65.67","url":null,"abstract":"| Cyst nematode, the Heterodera Schmidt (1871) is one of the most widely distributed and economically important plant parasitic nematode genus. During the present investigation of plant-parasitic nematode fauna from Gilgit-Baltistan, Pakistan, four cyst nematode species viz., Heterodera avenae Wollenweber, 1924; H. mani Mathews,1971; H. schachtii Schmidt, 1871 and H. zeae Koshy, Swarup and Sethi, 1971 were detected from all four districts.","PeriodicalId":207209,"journal":{"name":"Pakistan Journal of Nematology","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115109883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-08DOI: 10.17582/journal.pjn/2021/39.1.59.64
Aly Khan, Shagufta Ambreen Sheikh, K. A. Khanzada, S. Shaukat, J. Akhtar
| The study involves evaluation of two nematicides, namely Turtob-F (a new nematicide) and carbofuran on 3 nematode populations and two bacteria associated with the rhizosphere of brinjal. The effect of the nematicides was observed on the population size of the selected nematodes and bacteria as well as the yield of brinjal crop. All three nematode populations were markedly reduced by the applied nematicides. The bacterial populations (Pseudomonas and Klebsiella) however, remained uninfluenced by the nematicides. The yield of brinjal was found to be significantly elevated (p<0.05).
{"title":"Effect of Two Nematicides on Nematodes Populations and Two Bacterial Populations Associated with Brinjal (Solanum melongena L.) Crop","authors":"Aly Khan, Shagufta Ambreen Sheikh, K. A. Khanzada, S. Shaukat, J. Akhtar","doi":"10.17582/journal.pjn/2021/39.1.59.64","DOIUrl":"https://doi.org/10.17582/journal.pjn/2021/39.1.59.64","url":null,"abstract":"| The study involves evaluation of two nematicides, namely Turtob-F (a new nematicide) and carbofuran on 3 nematode populations and two bacteria associated with the rhizosphere of brinjal. The effect of the nematicides was observed on the population size of the selected nematodes and bacteria as well as the yield of brinjal crop. All three nematode populations were markedly reduced by the applied nematicides. The bacterial populations (Pseudomonas and Klebsiella) however, remained uninfluenced by the nematicides. The yield of brinjal was found to be significantly elevated (p<0.05).","PeriodicalId":207209,"journal":{"name":"Pakistan Journal of Nematology","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114795035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-27DOI: 10.17582/journal.pjn/2021/39.1.52.58
Khan Tamoor, Hafsa Hafsa, Hanif Maryam
| The present research study was undertaken to evaluate the nematicidal property of four indigenous medicinal plants viz., Ferula oopoda, Zataria multiflora, Achillea santolina and Nepeta cateria against Meloidogyne incognita at banana orchards of District Lasbela, Balochistan, Pakistan. Crude extracts, essential oils and fixed oil of these selected plants were tested separately. The crude extracts of Z. multiflora and F. oopoda showed higher mortality as compared to A. santolina and N. cateria. The results showed a maximum mortality of 76% by Z. multiflora and F. oopoda at highest dose of 3 g/ liter which was followed by 72% of N. cateria and 49% by A. santolina at the same dose. However, essential oils of A. santolina showed the highest mortality (79%) as compared to Z. multiflora (74%), F. oopoda (70%) and N. cateria (68%) at the same dose of 1.2 ml/ liter. While results of fixed oils showed maximum mortality of 74% by F. oopoda followed by 71%, 70% and 66% in Z. multiflora, A. santolina and N. cateria, respectively at a formulation of 3ml/ liter. A nematicide Furadan was kept as standard for efficacy comparison.
{"title":"Evaluation of Some Medicinal Plants for the Management of Root-Knot Diseases of Banana","authors":"Khan Tamoor, Hafsa Hafsa, Hanif Maryam","doi":"10.17582/journal.pjn/2021/39.1.52.58","DOIUrl":"https://doi.org/10.17582/journal.pjn/2021/39.1.52.58","url":null,"abstract":"| The present research study was undertaken to evaluate the nematicidal property of four indigenous medicinal plants viz., Ferula oopoda, Zataria multiflora, Achillea santolina and Nepeta cateria against Meloidogyne incognita at banana orchards of District Lasbela, Balochistan, Pakistan. Crude extracts, essential oils and fixed oil of these selected plants were tested separately. The crude extracts of Z. multiflora and F. oopoda showed higher mortality as compared to A. santolina and N. cateria. The results showed a maximum mortality of 76% by Z. multiflora and F. oopoda at highest dose of 3 g/ liter which was followed by 72% of N. cateria and 49% by A. santolina at the same dose. However, essential oils of A. santolina showed the highest mortality (79%) as compared to Z. multiflora (74%), F. oopoda (70%) and N. cateria (68%) at the same dose of 1.2 ml/ liter. While results of fixed oils showed maximum mortality of 74% by F. oopoda followed by 71%, 70% and 66% in Z. multiflora, A. santolina and N. cateria, respectively at a formulation of 3ml/ liter. A nematicide Furadan was kept as standard for efficacy comparison.","PeriodicalId":207209,"journal":{"name":"Pakistan Journal of Nematology","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130751933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-12DOI: 10.17582/JOURNAL.PJN/2020/38.2.171.178
M. S. Bajestani, E. M. Moghadam, R. Aghnoum, H. Rohani
{"title":"Study of Plant Parasitic Nematodes and Description of New Record (Rotylenchus alius) Associated with Barley (Hordium vulgare L.) in Khorasan Razavi Province, Northeast Iran","authors":"M. S. Bajestani, E. M. Moghadam, R. Aghnoum, H. Rohani","doi":"10.17582/JOURNAL.PJN/2020/38.2.171.178","DOIUrl":"https://doi.org/10.17582/JOURNAL.PJN/2020/38.2.171.178","url":null,"abstract":"","PeriodicalId":207209,"journal":{"name":"Pakistan Journal of Nematology","volume":"25 12","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"113931732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-30DOI: 10.18681/pjn.v38.i01.p15-24
M. Kajol
A population of Heterorhabditis (DH3) was isolated from agricultural fields from Meerut district, India by insect baiting technique. The isolate was identified as Heterorhabditis indica based on morpho-taxometrical and molecular analyses. The present populations were identical to the original description; however, the size of the 3 stages was longer than the topotype populations. Analysis of ITS-rDNA sequences showed 1 nucleotide base pair difference in aligned data with type description, however, no nucleotide base pair difference was seen in the D2D3 domain. The associated bacterial symbiont of the DH3 strain was identified as Photorhabdus akhurstii based on phenotypic, biochemical and 16S rDNA data. Most of the biochemical tests were negative and only three tests were found positive viz., Urease, Nitrogen reduction and Oxidase. Further, we evaluated its pathogenicity against Galleria mellonella and Helicoverpa armigera. The LD50 value of strain DH3 against G. mellonella larvae at 48 hours was 8 IJs/larva for 50% larval mortality whereas in Helicoverpa armigera it needed 13 IJs/larva, to kill 50% of the larvae.
{"title":"Biochemical and molecular characterization of Photorhabdus akhurstii associated with Heterorhabditis indica from Meerut, India","authors":"M. Kajol","doi":"10.18681/pjn.v38.i01.p15-24","DOIUrl":"https://doi.org/10.18681/pjn.v38.i01.p15-24","url":null,"abstract":"A population of Heterorhabditis (DH3) was isolated from agricultural fields from Meerut district, India by insect baiting technique. The isolate was identified as Heterorhabditis indica based on morpho-taxometrical and molecular analyses. The present populations were identical to the original description; however, the size of the 3 stages was longer than the topotype populations. Analysis of ITS-rDNA sequences showed 1 nucleotide base pair difference in aligned data with type description, however, no nucleotide base pair difference was seen in the D2D3 domain. The associated bacterial symbiont of the DH3 strain was identified as Photorhabdus akhurstii based on phenotypic, biochemical and 16S rDNA data. Most of the biochemical tests were negative and only three tests were found positive viz., Urease, Nitrogen reduction and Oxidase. Further, we evaluated its pathogenicity against Galleria mellonella and Helicoverpa armigera. The LD50 value of strain DH3 against G. mellonella larvae at 48 hours was 8 IJs/larva for 50% larval mortality whereas in Helicoverpa armigera it needed 13 IJs/larva, to kill 50% of the larvae.","PeriodicalId":207209,"journal":{"name":"Pakistan Journal of Nematology","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125407160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-30DOI: 10.18681/pjn.v38.i01.p45-55
Shahid Ahmed
{"title":"Evaluation of media for mass production and fermentation of Pseudomonas spp. for integrated management of cereal cyst nematode (Heterodera avenae)","authors":"Shahid Ahmed","doi":"10.18681/pjn.v38.i01.p45-55","DOIUrl":"https://doi.org/10.18681/pjn.v38.i01.p45-55","url":null,"abstract":"","PeriodicalId":207209,"journal":{"name":"Pakistan Journal of Nematology","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114884030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-30DOI: 10.18681/pjn.v38.i01.p93-101
K. Abbasi
{"title":"Chitinase activity of biocontrol fungi cultured from the golden potato cyst nematode, Globodera rostochiensis","authors":"K. Abbasi","doi":"10.18681/pjn.v38.i01.p93-101","DOIUrl":"https://doi.org/10.18681/pjn.v38.i01.p93-101","url":null,"abstract":"","PeriodicalId":207209,"journal":{"name":"Pakistan Journal of Nematology","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116389311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-30DOI: 10.18681/pjn.v38.i01.p1-13
E. Iqbal
{"title":"Description of Discolaimus miniodontii n.sp. and Laevides hunderansis n. sp. with notes on Discolaimoides spatilabium (Nematoda: Dorylaimida) from District Ghizer, Gilgit-Baltistan, Pakistan","authors":"E. Iqbal","doi":"10.18681/pjn.v38.i01.p1-13","DOIUrl":"https://doi.org/10.18681/pjn.v38.i01.p1-13","url":null,"abstract":"","PeriodicalId":207209,"journal":{"name":"Pakistan Journal of Nematology","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123449542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-30DOI: 10.18681/pjn.v38.i01.p57-65
U. A. Al-Karim
Greenhouse experiments were conducted to reveal the effectiveness of potential biological control products to manage and evaluate the development of Rotylenchulus reniformis on cotton. Tests included seeds treated with the Abamectin, ILeVo, and a non-treated control. Results showed that seed treatments with Abamectin and Burkholderia sp. suppressed the numbers of R. reniformis eggs significantly. Seeds treated with Abamectin and bacteria had fewer vermiform adults in the soil in comparison with the non-treated seeds. The bacteria and Burkholderia sp. seeds treatments drastically suppressed the number of eggs isolated from cotton roots compared with the non-treated control. Abamectin also inhibited the number of vermiform life-stages found in the soil as compared to the non-treated control. Biological seed treatments produced no negative effects on plant growth. The use of different biological control as seed treatments can manage plantparasitic nematodes and limit damage.
{"title":"Evaluation of biological seed treatments for management of Rotylenchulus reniformis on cotton","authors":"U. A. Al-Karim","doi":"10.18681/pjn.v38.i01.p57-65","DOIUrl":"https://doi.org/10.18681/pjn.v38.i01.p57-65","url":null,"abstract":"Greenhouse experiments were conducted to reveal the effectiveness of potential biological control products to manage and evaluate the development of Rotylenchulus reniformis on cotton. Tests included seeds treated with the Abamectin, ILeVo, and a non-treated control. Results showed that seed treatments with Abamectin and Burkholderia sp. suppressed the numbers of R. reniformis eggs significantly. Seeds treated with Abamectin and bacteria had fewer vermiform adults in the soil in comparison with the non-treated seeds. The bacteria and Burkholderia sp. seeds treatments drastically suppressed the number of eggs isolated from cotton roots compared with the non-treated control. Abamectin also inhibited the number of vermiform life-stages found in the soil as compared to the non-treated control. Biological seed treatments produced no negative effects on plant growth. The use of different biological control as seed treatments can manage plantparasitic nematodes and limit damage.","PeriodicalId":207209,"journal":{"name":"Pakistan Journal of Nematology","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114686184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-30DOI: 10.18681/pjn.v38.i01.p67-80
El-Nuby A. S. M
{"title":"Control of root-knot nematodes using wild plants colonized Sinai, Egypt","authors":"El-Nuby A. S. M","doi":"10.18681/pjn.v38.i01.p67-80","DOIUrl":"https://doi.org/10.18681/pjn.v38.i01.p67-80","url":null,"abstract":"","PeriodicalId":207209,"journal":{"name":"Pakistan Journal of Nematology","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133909879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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