ON THE HEMOTOXIN OF THE SARCOSPORIDIA

1. Sarcosporidiotoxin contains hemolytic and hemagglutinative principles.2. The optimal concentration of the erythrocytal suspension to test both of these reactions is 2.5-5.0%.3. The physico-chemical characteristics of these two principles of the toxin: Hemolytic principle is hardly soluble in water, saline and glycerinated saline, but the hemagglutinative principle is soluble in these media; the one is soluble in alcohol and ether, but the other insoluble; The one is thermostable, but the other is destroyed by heating at 85°C. for 1/2 hour; The one is negative to protein reaction test, but the other positive; Concerning their resistances against acid and alkali, dialysis and adsorption, both of them almost agreed with each other.4. The normal serum acts indifferently up to a certain amount but above this limit, it rather inhibits hemolysis by the hemolytic principle of the Sarcosporidiotoxin.5. Alcohol soluble fraction of the serum (or lecithin fraction) accelerates hemolysis by this toxin, but the insoluble fraction (or albumin fraction) and globulin fraction inhibit hemagglutinative action of the toxin.6. It might be justifiable to conclude from the experimental data that the hemolysis inhibitory substance (albumin fraction) acts directly against hemolytic principle while the hemagglutination inhibitory substance (globulin fraction) first combines with the erythrocytes and consequently inhibits hemagglutination.7. All the batches of the immune sera prepared with the sarcosporidiotoxin treated by divergent methods were indifferent to hemagglutination.8. The serum of the rabbit immunized with the alcoholic extracts of the sarcosporidiotoxin, either simple or in mixture with the pig's serum, was indifferent in hemagglutination and hemolysis, but it neutralized the toxic principle.