植物化学筛选分析、总表型黄素和抗氧化活性泥炭沼泽(菊苣caju提取物,roxb)

Raden Roro Ariessanty Alicia Kusuma Wardhani, Okviyoandra Akhyar, Emilda Prasiska
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引用次数: 4

摘要

森林galam是加里曼丹南部典型的森林,能够生长在泥炭上,有潜力成为草本植物。树皮galam是未充分利用的部分植物。因此,本文研究了树皮中所含的复合生物活性因子,使树皮具有更广泛的药用价值。筛选植物化学分析,总酚类黄酮和抗氧化活性对提取乙醇树皮galam。植物化学筛选结果表明,树皮提取物的次生代谢产物主要为生物碱、多酚、类黄酮和醌。采用Folin-Ciocalteu法测定总酚含量,测定结果为33,8646 ppm,总酚含量为30,47814 mg GAE/g。在373,6 nm处用氯化铝法测定了总黄酮的含量。结果表明,黄酮类化合物总浓度为0.0942 ppm,总黄酮含量为0.2826 mg QE/g。抗氧化活性测定采用DPPH(1,1-二苯基-2-苦基肼)法,紫外-可见分光光度法测定,波长518、90 nm。所使用的比较化合物是抗坏血酸。抗氧化活性测试表明,树皮提取物的ic50值分别为44、4888 ppm。甘兰提取物树皮的抗氧化强度包括在非常强的强度(<50 ppm)。
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ANALISIS SKRINING FITOKIMIA, KADAR TOTAL FENOL-FLAVONOID DAN AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL KULIT KAYU TANAMAN GALAM RAWA GAMBUT (Melaleuca cajuputi roxb)
Forest galam is forest typical south kalimantan capable of growing on land peat and have the potential as herbs. Bark galam is part plant which remains underutilized. Hence the study was done to know compound bioaktif which is found in bark galam so bark galam could be more used especially as herbs. Screening phytochemistry analysis, the total fenol-flavonoid and the antioxidant activity performed on extract ethanol bark galam. Based on the results of phytochemical screening, compounds of secondary metabolite contained in the bark extract were alkaloid, polyphenol, flavonoid and quinone. Total phenolic was defined by using Folin-Ciocalteu method at wavelengths 748 nm and the results was 33,8646 ppm with the total of phenol  level was 30,47814 mg GAE/g of extract. The total flavonoid was specified by using the method of AlCl 3 at 373,6 nm. The results showed that the total concentration of flavonoid 0,0942 ppm with the total level of extract flavonoid was 0,2826 mg QE/g of extract. The antioxidant activity test uses DPPH (1,1-diphenyl-2- picrylhidrazyl) method and the measurement uses spectrophotometry UV-Vis at wavelengths 518,90 nm. The comparison compound used is ascorbic acid. Antioxidant activity test showed that the IC 50 values in the bark extract is 44,4888 ppm. Antioxidant intensity in the bark of galam extract is included in very strong intensity (<50 ppm).
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