不同盐度和光照强度对黄斑卟啉生长的影响

Leyla Uslu
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摘要

在实验室条件下,分别在20±2℃、16:8(亮:暗)光周期、不同盐度(20‰、30‰、40‰)和两种不同光强(37µmol m-2s-1光子和110µmol m-2s-1光子)连续曝气条件下培养cruentum卟啉(Porphyridium cruentum),并测定其生长情况。用干物质、光密度和叶绿素a参数测定生长。在110µmol m-2s-1光子光强度下,盐度为30‰的培养基生长最佳。该组光密度(OD)为1.504±0.003,干物质量为1.327gl-1。在37µmolµmol m-2s-1光子光强的情况下,30‰和50‰盐度组的光密度值相似,分别为1.234±0.004和1.215±0.002。干物质的数量也相似;分别为1.168和1.159gl-1。而在37µmol m-2s-1光子光强和20‰盐度条件下,生长最低。该组最后一天的光密度为1.165±0.004,测定干物质量为0.986gl-1。在37µmol m-2s-1光子光强下测定各组叶绿素a的含量。
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EFFECT OF DIFFERENT SALINITY AND LIGHT INTENSITY ON THE GROWTH OF PORPHYRIDIUM CRUENTUM
In the study, Porphyridium cruentum was cultured under laboratory conditions at 20±2°C, 16:8 (light:dark) photoperiod and continuous aeration to different salinity (20‰, 30‰, 40‰) and two different light intensities (37 µmol m-2s-1 photon and 110 µmol m-2s-1 photon) and growth was determined. Dry matter, optical density and chlorophyll a parameter were used to determine growth. The best growth was determined in culture with a salinity of 30‰ at 110 µmol m-2s-1 photon light intensity. In this group, the optical density (OD) was 1.504±0.003 and the dry matter amount was 1.327gl-1. In the case of 37µmol µmol m-2s-1 photon light intensity, the optical density values were found to be similar in groups with 30‰ and 50‰ salinity and were found to be 1.234±0.004 and 1.215±0.002, respectively. The amounts of dry matter were also similar; 1.168gl-1 and 1.159gl-1, respectively. While the lowest growth was in the culture at 37 µmol m-2s-1 photon light intensity and 20‰ salinity. The optical density obtained on the last day of this group was 1.165±0.004 and the dry matter amount was determined as 0.986gl-1. The amount of chlorophyll a was determined in the cultured groups at the best 37 µmol m-2s-1 photon light intensity.
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