高韧性蜘蛛丝纤维由哺乳动物细胞产生的可溶性重组丝纺成

Biopolymers Online Pub Date : 2003-01-27 DOI:10.1002/3527600035.BPOL8005

C. Karatzas, N. Chrétien, F. Duguay, Annie Bellemare, J. Zhou, A. Rodenhiser, Shafiul Islam, C. Turcotte, Yue-Jin Huang, A. Lazaris

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引用次数: 12

摘要

rc-蜘蛛丝蛋白的生产历史概述可溶性rc-蜘蛛丝蛋白在哺乳动物细胞中的过表达rc-拖丝蜘蛛丝蛋白的分泌rc-蜘蛛丝蛋白的大规模生产rc-蜘蛛丝蛋白的纯化和特性rc-蜘蛛丝蛋白在转基因动物的乳汁中生产rc-蜘蛛丝蛋白转基因小鼠的产生为蜘蛛拖丝基因的乳腺生产蜘蛛拖丝蛋白重组蛛丝基纤维纺丝纺浆料制备、纤维纺丝及表征纤维纺丝后处理展望丝纤维的形成及rc-蛛丝纤维的纺丝工艺应用及专利展望基因工程;重组丝蛋白;纤维纺纱;的表情;哺乳动物细胞;转基因小鼠;自旋涂料;纤维

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High‐toughness Spider Silk Fibers Spun from Soluble Recombinant Silk Produced in Mammalian Cells

Introduction Historical Outline Production of rc-Spider Silk Proteins Over-expression of Soluble rc-Spider silk Proteins in Mammalian Cells Secretion of rc-Dragline Spider Silk proteins Large-scale Production of rc-Spider Silk Proteins Purification and Characterization of rc-Spider Silk Proteins Production of rc-Spider Silk Proteins in the Milk of Transgenic Animals Generation of Transgenic Mice for the Spider Dragline Silk Genes Mammary Gland Production of Spider Dragline Silk Proteins Recombinant Spider Silk-based Fiber Spinning Spin Dope Preparation, Fiber Spinning and Characterization Fiber Post-Spinning Treatment Perspective on the Formation of Silk Fibers and the Spinning Process Applications of rc-Spider Silk Fibers, and Prospects Patents Chronological Summary of Patents Conclusions Acknowledgments Keywords: spider silk; genetic engineering; recombinant silk protein; fiber spinning; expression; mammalian cells; transgenic mice; spin dope; fibers

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