蓝光LED光对金鸡纳体外培养的生长有促进作用

Oscar Vivanco-Galván, Yuliana Jiménez-Gaona, D. Castillo, Hernán Lucero
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引用次数: 2

摘要

金鸡纳(chinona officinalis L.)是历史上最重要的药用植物之一,抗疟疾药物奎宁(quinine)就是从这种植物中提取的。它目前是濒临灭绝的物种。因此,本文采用体外培养技术对该物种进行了繁殖,并在控制条件下评价了人工光对Officinalis L.生理发育的影响。因此,本研究确定了蓝色led光对金鸡纳生长和芽数的促进作用。将金鸡纳离体培养在Murashige和Skoog (MS)培养基上,在白色(对照)和蓝色发光二极管(led)光下培养。8周后,测定了山药的生长和芽数。有趣的是,与对照相比,蓝光处理增加了山药的茎长和芽数。在Officinalis L离体繁殖过程中加入蓝光是提高植株品质的有效途径。未来的研究方向可能包括蓝光对体外培养的Officinalis L.次生代谢产物的产生、抗氧化酶的活性和蛋白质表达的影响。
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Blue LED light enhances the growth of Cinchona officinalis L. cultured in vitro
Cinchona officinalis L. is one of the most important and historically medicinal plants from which the antimalarial drug known as quinine is extracted. It is currently an endangered species. Thus, in vitro culture techniques are applied to propagate the species and to evaluate the effect of artificial light on the physiological development of C. Officinalis L. under controlled conditions. In that sense, the current study has determined the impact of blue led light on the enhancement of growth and number of shoots of Cinchona officinalis L. In vitro explants of C. Officinalis L were cultured on Murashige and Skoog (MS) medium and cultured under the white (control) and blue light-emitting diodes (LED) light. After eight weeks, growth and bud numbers were determined in C. officinalis L. Interestingly, blue light treatment increased the shoot length and bud numbers in comparison with the control. Incorporating blue light during in vitro propagation of C. Officinalis L can be a beneficial way to increase plant quality. Future perspectives could include the impact of blue light on the production of secondary metabolites, activities of antioxidant enzymes, and protein expression of in vitro-grown C. Officinalis L.
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