Isolation and structural determination of ursane-type triterpene from Neolamarckia cadamba dried leaves

This article documents the first time isolation of Carissic acid from Neolamarckia cadamba (Roxb.) Bosser (Family: Rubiaceae) leaves. This bioactive triterpenic acid was isolated with a yield of 3.26% by ultrasonic waves. One-dimensional Nuclear Magnetic Resonance (NMR) spectroscopic techniques such as proton NMR (1H NMR), carbon-13 NMR (13C NMR), distortionless enhancement by polarisation transfer (DEPT), and two-dimensional NMR spectroscopic methods such as heteronuclear single quantum coherence (HSQC) were used to identify the structure. About 63.0 mg of pure carissic acid was isolated from 1.931 grams of acetone extract using sonomaceration as an extraction technique.