Nista Gurung, Manoj Sharma, Ashma Gurung, Santosh Chaudhary, H. P. Devkota, S. Panta, H. Paudel, Atisammodavardhana Kaundinnyayana, Khem Raj Joshi
Numerous medicinal plants have been used traditionally to treat, relieve, and prevent various diseases since ancient time, yet many of them are to be scientifically investigated. The present study was carried out with the aim of phytochemical screening, determination of 1,1–diphenyl–2–picrylhydrazyl (DPPH) free radical scavenging activity and antibacterial activity of 70% methanolic extracts of six medicinal plants of Nepal used by different ethnic tribes, and to isolate chemical compounds from one of them. Agar well diffusion method was used to assess the in vitro antibacterial activity, whereas the chemical isolation was carried out using different chromatographic techniques. Then, the structure of the isolated compounds was determined by using NMR (Nuclear Magnetic Resonance) spectroscopic method, followed by spectral analysis in comparison with published data. Of the six extracts, the extracts of Angiopteris helferiana and Drynaria coronans showed moderate antibacterial activities. Moreover, the extract of Dischidia bengalensis showed the most potent antioxidant activity and was thus subjected to chromatographic separations utilizing different chromatographic principles to isolate 2"-O- rhamnosylvitexin (1) and isovitexin (2). These secondary metabolites are reported for the first time from this plant whilst, their pharmacological activity is yet to be screened.
{"title":"Phytochemical Analysis and Bioactivity of Nepalese Medicinal Plants, with Compound Isolation from Dischidia bengalensis Colebr.","authors":"Nista Gurung, Manoj Sharma, Ashma Gurung, Santosh Chaudhary, H. P. Devkota, S. Panta, H. Paudel, Atisammodavardhana Kaundinnyayana, Khem Raj Joshi","doi":"10.53365/nrfhh/188221","DOIUrl":"https://doi.org/10.53365/nrfhh/188221","url":null,"abstract":"Numerous medicinal plants have been used traditionally to treat, relieve, and prevent various diseases since ancient time, yet many of them are to be scientifically investigated. The present study was carried out with the aim of phytochemical screening, determination of 1,1–diphenyl–2–picrylhydrazyl (DPPH) free radical scavenging activity and antibacterial activity of 70% methanolic extracts of six medicinal plants of Nepal used by different ethnic tribes, and to isolate chemical compounds from one of them. Agar well diffusion method was used to assess the in vitro antibacterial activity, whereas the chemical isolation was carried out using different chromatographic techniques. Then, the structure of the isolated compounds was determined by using NMR (Nuclear Magnetic Resonance) spectroscopic method, followed by spectral analysis in comparison with published data. Of the six extracts, the extracts of Angiopteris helferiana and Drynaria coronans showed moderate antibacterial activities. Moreover, the extract of Dischidia bengalensis showed the most potent antioxidant activity and was thus subjected to chromatographic separations utilizing different chromatographic principles to isolate 2\"-O- rhamnosylvitexin (1) and isovitexin (2). These secondary metabolites are reported for the first time from this plant whilst, their pharmacological activity is yet to be screened.","PeriodicalId":394827,"journal":{"name":"Natural Resources for Human Health","volume":" 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141372307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Claudia Mancuello, Yanine Maubet, Enzo Cristaldo, Brenda Veloso, G. Robledo, Angela Traba, Luis Marín, Elvio Gayoso, Michele G. Campi
In this work, we evaluated the antioxidant, toxicological, mutagenic, antigenotoxic and nutritional properties of Oudemansiella cubensis, which is a mushroom found in neotropical regions worldwide and native to Paraguay. Nutritional content analyses revealed that O. cubensis is a rich source of protein, dietary fiber, and fats. Antimicrobial analyses showed antimicrobial activity against Pseudomonas aeruginosa and Enterococcus faecalis. Additionally, the species resulted to be nontoxic for human consumption with an LD50 value of 37.1 mg.mL-1. The ethanolic extract of O. cubensis showcased an important antimutagenic activity at a concentration of 20 mg.mL-1, which promotes the prevention of genotoxic damage. Regarding its chemical profile, Gas Chromatography – Mass Spectrometry confirmed the presence of compounds such as l-(+)-ascorbic acid 2,6-dihexadecanoate, octacosanol and cyclo(l-leucyl-l-prolyl), which stood out for antioxidant and antimicrobial properties. This study provided further evidence that Oudemansiella cubensis is a valuable species because of its potential for biotechnological applications.
{"title":"Oudemansiella cubensis an Edible Mushroom from the Neotropics with Biological and Nutritional Benefits","authors":"Claudia Mancuello, Yanine Maubet, Enzo Cristaldo, Brenda Veloso, G. Robledo, Angela Traba, Luis Marín, Elvio Gayoso, Michele G. Campi","doi":"10.53365/nrfhh/189170","DOIUrl":"https://doi.org/10.53365/nrfhh/189170","url":null,"abstract":"In this work, we evaluated the antioxidant, toxicological, mutagenic, antigenotoxic and nutritional properties of Oudemansiella cubensis, which is a mushroom found in neotropical regions worldwide and native to Paraguay. Nutritional content analyses revealed that O. cubensis is a rich source of protein, dietary fiber, and fats. Antimicrobial analyses showed antimicrobial activity against Pseudomonas aeruginosa and Enterococcus faecalis. Additionally, the species resulted to be nontoxic for human consumption with an LD50 value of 37.1 mg.mL-1. The ethanolic extract of O. cubensis showcased an important antimutagenic activity at a concentration of 20 mg.mL-1, which promotes the prevention of genotoxic damage. Regarding its chemical profile, Gas Chromatography – Mass Spectrometry confirmed the presence of compounds such as l-(+)-ascorbic acid 2,6-dihexadecanoate, octacosanol and cyclo(l-leucyl-l-prolyl), which stood out for antioxidant and antimicrobial properties. This study provided further evidence that Oudemansiella cubensis is a valuable species because of its potential for biotechnological applications.","PeriodicalId":394827,"journal":{"name":"Natural Resources for Human Health","volume":" 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141370915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Menga Mukolo Pisco, Gerengbo Koto Georges, M. Monizi, Masengo Ashande Colette, Tshimankinda Mpiana Pius, Mudogo Virima, K. Ngbolua
The research conducted an investigation into the utilization of medicinal plants for treating influenza, while also employing molecular modeling to scientifically validate their traditional applications. Informants were selected from IBI-Village and its environs using a snowball sampling technique. Data collection utilized semi-structured questionnaires and interviews. Consensus among informants and the cultural significance of the plants were assessed. A total of 40 plants employed in traditional influenza care were identified, including Cymbopogon citratus, Ocimum gratissimum, Tetradenia riparia, Eucalyptus globulus, Citrus limon, Zingiber officinalis, and Lippia multiflora. The majority of these plants were shrubs and trees. Analysis revealed that marital status influenced only the mode of recipe administration. Respondents were categorized based on ethnomedical practices: some were prescribed based on plant condition and parts used, while others focused on administration mode, preparation method, and form of use. Molecular docking demonstrated that selected compounds formed stable complexes, categorized into five classes: Neral and Eugenol (Class 1), Fenchone and Oseltamivir (Class 2), Eucalyptol and 1,8-Cineole (Class 3), (+)-Limonene (Class 4), and Zingiberene (Class 5). Neral and Eugenol formed two and four hydrogen bonds with the receptor, respectively. Molecular dynamics simulations showed around 300 amino acid residues interacting with the ligand, with specific residues like Arg_706, Val_709, Ser_712, and Arg_721 forming hydrogen bonds with Eugenol. The integration of molecular dynamics, molecular docking, and ethnobotany highlighted the potential efficacy of C. citratus and O. gratissimum against influenza at the molecular level. Formulating pharmaceuticals based on essential oils from these plants could enhance influenza management.
该研究对利用药用植物治疗流感进行了调查，同时还利用分子模型对其传统应用进行了科学验证。研究人员采用滚雪球式抽样技术，从 IBI 村及其周边地区选取了信息提供者。数据收集采用了半结构化问卷和访谈的方式。对信息提供者之间的共识和植物的文化意义进行了评估。共确定了 40 种用于传统流感护理的植物，包括枸橼酸香蒲（Cymbopogon citratus）、广藿香（Ocimum gratissimum）、四季青（Tetradenia riparia）、蓝桉（Eucalyptus globulus）、柠檬（Citrus limon）、细辛（Zingiber officinalis）和多花菩提（Lippia multiflora）。这些植物大部分是灌木和乔木。分析表明，婚姻状况只影响配方的使用方式。根据民族医药实践对受访者进行了分类：一些受访者根据植物状况和使用部位开具处方，而另一些受访者则侧重于给药方式、制备方法和使用形式。分子对接表明，所选化合物形成了稳定的复合物，可分为五类：Neral和丁香酚（第1类）、葑酮和奥司他韦（第2类）、桉叶油醇和1,8-蒎烯（第3类）、(+)-柠檬烯（第4类）和辛夷烯（第5类）。Neral 和 Eugenol 分别与受体形成了两个和四个氢键。分子动力学模拟显示，约有 300 个氨基酸残基与配体相互作用，其中 Arg_706、Val_709、Ser_712 和 Arg_721 等特定残基与丁香酚形成氢键。分子动力学、分子对接和人种植物学的结合，从分子水平上凸显了 C. citratus 和 O. gratissimum 对流感的潜在功效。以这些植物的精油为基础配制药物，可以提高流感防治效果。
{"title":"Integrating Ethnobotany and Artificial intelligence to validate the potential bioactivity of Two Medicinal Plants Traditionally used in the treatment of influenza in IBI-Village and surrounding areas, Democratic Republic of the Congo","authors":"Menga Mukolo Pisco, Gerengbo Koto Georges, M. Monizi, Masengo Ashande Colette, Tshimankinda Mpiana Pius, Mudogo Virima, K. Ngbolua","doi":"10.53365/nrfhh/187385","DOIUrl":"https://doi.org/10.53365/nrfhh/187385","url":null,"abstract":"The research conducted an investigation into the utilization of medicinal plants for treating influenza, while also employing molecular modeling to scientifically validate their traditional applications. Informants were selected from IBI-Village and its environs using a snowball sampling technique. Data collection utilized semi-structured questionnaires and interviews. Consensus among informants and the cultural significance of the plants were assessed. A total of 40 plants employed in traditional influenza care were identified, including Cymbopogon citratus, Ocimum gratissimum, Tetradenia riparia, Eucalyptus globulus, Citrus limon, Zingiber officinalis, and Lippia multiflora. The majority of these plants were shrubs and trees. Analysis revealed that marital status influenced only the mode of recipe administration. Respondents were categorized based on ethnomedical practices: some were prescribed based on plant condition and parts used, while others focused on administration mode, preparation method, and form of use. Molecular docking demonstrated that selected compounds formed stable complexes, categorized into five classes: Neral and Eugenol (Class 1), Fenchone and Oseltamivir (Class 2), Eucalyptol and 1,8-Cineole (Class 3), (+)-Limonene (Class 4), and Zingiberene (Class 5). Neral and Eugenol formed two and four hydrogen bonds with the receptor, respectively. Molecular dynamics simulations showed around 300 amino acid residues interacting with the ligand, with specific residues like Arg_706, Val_709, Ser_712, and Arg_721 forming hydrogen bonds with Eugenol. The integration of molecular dynamics, molecular docking, and ethnobotany highlighted the potential efficacy of C. citratus and O. gratissimum against influenza at the molecular level. Formulating pharmaceuticals based on essential oils from these plants could enhance influenza management.","PeriodicalId":394827,"journal":{"name":"Natural Resources for Human Health","volume":" 24","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141375174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aswathi K Biju, Rahul Raj Chennam Lakshmikumar, Kannan R. R. Rengasamy
ATP Citrate Lyase (ACLY) is a central enzyme bridging carbohydrate metabolism and lipid biosynthesis, crucial for various physiological processes and implicated in numerous pathologies. This review comprehensively describes ACLY, a pivotal enzyme at lipid biosynthesis and cellular metabolism interface. It serves as crucial role for several physiological functions, making it a highly valued topic for research. Starting with a description of the biochemistry and molecular biology of ACLY, elucidating its structural features, functions, and regulatory mechanisms, particularly in lipid synthesis and histone acetylation. Considering the pathophysiological aspect, the review also examines the relationship between ACLY dysregulation and associated diseases, explaining its contribution to pathological conditions mechanistically. An explanation of ACLY inhibitors is as follows: an overview of these inhibitors, an understanding of their mechanisms of action, and an analysis of their effectiveness and specificity. The following sections transition from preclinical studies, summarising key findings and knowledge into therapeutic potential, to clinical trials. The review offers updated information about ACLY research, integrating data from multiple sources to give an in-depth understanding. It concludes with the challenges and outlook facing the developing ACLY inhibitors, considering the strategies to overcome these challenges and the upcoming path in research and development in this promising field. This comprehensive examination not only encapsulates the knowledge at present but also aims to inspire and guide future inquiries into the complex realm of ACLY and its potential as a therapeutic target, ultimately improving patient outcomes and quality of life.
{"title":"ATP-Citrate Lyase (ACLY): An Extensive Investigation from Molecular Insight to Therapeutic implications","authors":"Aswathi K Biju, Rahul Raj Chennam Lakshmikumar, Kannan R. R. Rengasamy","doi":"10.53365/nrfhh/189500","DOIUrl":"https://doi.org/10.53365/nrfhh/189500","url":null,"abstract":"ATP Citrate Lyase (ACLY) is a central enzyme bridging carbohydrate metabolism and lipid biosynthesis, crucial for various physiological processes and implicated in numerous pathologies. This review comprehensively describes ACLY, a pivotal enzyme at lipid biosynthesis and cellular metabolism interface. It serves as crucial role for several physiological functions, making it a highly valued topic for research. Starting with a description of the biochemistry and molecular biology of ACLY, elucidating its structural features, functions, and regulatory mechanisms, particularly in lipid synthesis and histone acetylation. Considering the pathophysiological aspect, the review also examines the relationship between ACLY dysregulation and associated diseases, explaining its contribution to pathological conditions mechanistically. An explanation of ACLY inhibitors is as follows: an overview of these inhibitors, an understanding of their mechanisms of action, and an analysis of their effectiveness and specificity. The following sections transition from preclinical studies, summarising key findings and knowledge into therapeutic potential, to clinical trials. The review offers updated information about ACLY research, integrating data from multiple sources to give an in-depth understanding. It concludes with the challenges and outlook facing the developing ACLY inhibitors, considering the strategies to overcome these challenges and the upcoming path in research and development in this promising field. This comprehensive examination not only encapsulates the knowledge at present but also aims to inspire and guide future inquiries into the complex realm of ACLY and its potential as a therapeutic target, ultimately improving patient outcomes and quality of life.","PeriodicalId":394827,"journal":{"name":"Natural Resources for Human Health","volume":"60 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141382980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bisma Jan, Sameer Ahmad, Mohammad Ibrahim, Bharti Choudhary
Polycystic Ovary Syndrome (PCOS) is the primary endocrine disorder whose cause remains unidentified but is widely prevalent. This intricate condition affects a significant portion of women of reproductive age, affecting approximately 17.8% of them. While dietary approaches have been explored as the initial method of treatment for individuals with PCOS, there is no definitive consensus on the most effective diet. There is limited evidence to recommend a specific diet composition for PCOS with approaches including modifying protein, carbohydrate or fat quality or quantity generally having similar effects on the presentations of PCOS. It is crucial to recognize that maintaining a proper diet and ensuring sufficient nutritional status are pivotal factors in preventing the development of PCOS. In this comprehensive review, we explored various aspects of PCOS, ranging from its molecular pathogenesis to therapeutic dietary innovations and specific therapeutic interventions. Understanding the molecular pathogenesis of PCOS is vital, and we delve into the intricate mechanisms involved in its development. We examined the interplay between PCOS and metabolic conditions, such as insulin resistance and dyslipidemia, highlighting the interconnectedness of these aspects and their impact on the clinical presentation and management of the syndrome. While the optimal diet remains a subject of ongoing research, we emphasize on the critical role of proper diet and its potential in the avoidance and management of PCOS. Additionally, we reviewed specific dietary components, such as curcumin and green tea, and their potential therapeutic benefits in PCOS management.
{"title":"Nutritional Management of Polycystic Ovary Syndrome: Insights into Diet and Nutrition Strategies","authors":"Bisma Jan, Sameer Ahmad, Mohammad Ibrahim, Bharti Choudhary","doi":"10.53365/nrfhh/183616","DOIUrl":"https://doi.org/10.53365/nrfhh/183616","url":null,"abstract":"Polycystic Ovary Syndrome (PCOS) is the primary endocrine disorder whose cause remains unidentified but is widely prevalent. This intricate condition affects a significant portion of women of reproductive age, affecting approximately 17.8% of them. While dietary approaches have been explored as the initial method of treatment for individuals with PCOS, there is no definitive consensus on the most effective diet. There is limited evidence to recommend a specific diet composition for PCOS with approaches including modifying protein, carbohydrate or fat quality or quantity generally having similar effects on the presentations of PCOS. It is crucial to recognize that maintaining a proper diet and ensuring sufficient nutritional status are pivotal factors in preventing the development of PCOS. In this comprehensive review, we explored various aspects of PCOS, ranging from its molecular pathogenesis to therapeutic dietary innovations and specific therapeutic interventions. Understanding the molecular pathogenesis of PCOS is vital, and we delve into the intricate mechanisms involved in its development. We examined the interplay between PCOS and metabolic conditions, such as insulin resistance and dyslipidemia, highlighting the interconnectedness of these aspects and their impact on the clinical presentation and management of the syndrome. While the optimal diet remains a subject of ongoing research, we emphasize on the critical role of proper diet and its potential in the avoidance and management of PCOS. Additionally, we reviewed specific dietary components, such as curcumin and green tea, and their potential therapeutic benefits in PCOS management.","PeriodicalId":394827,"journal":{"name":"Natural Resources for Human Health","volume":"14 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140081219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shreya Nair, Tina Sharma, Rohith Krishna, Archana Gautam, M. S. Sankhla
Opium is a psychoactive substance, that is often involved in drug trafficking, drug abuse, and various other crimes. Its ability to induce sleep and relieve pain makes it prone to its consumption. This opium and its byproducts when consumed, are bound by the opium receptors, namely, alpha, delta, and mu receptors. Mu-opioid receptors (MOR) are involved in the pain-relieving mechanism in the human body. Therefore, molecular docking was performed to dock MOR with various natural and synthetic opioids with the help of software tools Python, MGL Tools, AutoDock, and Babel. With the help of the software Discovery Studio, the result was analyzed. The study showed the synthetic opioids Buprenorphine, Hydromorphone, and Oxymorphone with the lowest binding energy of -9.10, -8.39, and -8.39 respectively. ADME analysis of the drugs was done for better understanding by the SwissADME tool and was found that Oxycodone, Oxymorphone, Remifentanil, and Noscapine were found to be more absorbed in the intestines whereas, all the others were found to be permeable by the Blood-Brain Barrier.
鸦片是一种精神活性物质，经常涉及贩毒、吸毒和其他各种犯罪。鸦片具有诱导睡眠和缓解疼痛的作用，因此很容易被人吸食。鸦片及其副产品在吸食后会与鸦片受体结合，即α、δ和μ受体。缪阿片受体（MOR）参与了人体的止痛机制。因此，在 Python、MGL Tools、AutoDock 和 Babel 等软件工具的帮助下，对 MOR 与各种天然和合成阿片类药物进行了分子对接。在 Discovery Studio 软件的帮助下，对结果进行了分析。研究结果表明，合成阿片类药物丁丙诺啡、氢吗啡酮和氧吗啡酮的结合能最低，分别为-9.10、-8.39和-8.39。研究发现，羟考酮、羟吗啡酮、雷米芬太尼和莨菪碱在肠道中的吸收率较高，而其他药物在血脑屏障中的渗透率较低。
{"title":"An In-Silico Study on the Molecular Docking of Various Natural and Synthetic Opioids to Mu-Opioid Receptors in Homo sapiens","authors":"Shreya Nair, Tina Sharma, Rohith Krishna, Archana Gautam, M. S. Sankhla","doi":"10.53365/nrfhh/181166","DOIUrl":"https://doi.org/10.53365/nrfhh/181166","url":null,"abstract":"Opium is a psychoactive substance, that is often involved in drug trafficking, drug abuse, and various other crimes. Its ability to induce sleep and relieve pain makes it prone to its consumption. This opium and its byproducts when consumed, are bound by the opium receptors, namely, alpha, delta, and mu receptors. Mu-opioid receptors (MOR) are involved in the pain-relieving mechanism in the human body. Therefore, molecular docking was performed to dock MOR with various natural and synthetic opioids with the help of software tools Python, MGL Tools, AutoDock, and Babel. With the help of the software Discovery Studio, the result was analyzed. The study showed the synthetic opioids Buprenorphine, Hydromorphone, and Oxymorphone with the lowest binding energy of -9.10, -8.39, and -8.39 respectively. ADME analysis of the drugs was done for better understanding by the SwissADME tool and was found that Oxycodone, Oxymorphone, Remifentanil, and Noscapine were found to be more absorbed in the intestines whereas, all the others were found to be permeable by the Blood-Brain Barrier.","PeriodicalId":394827,"journal":{"name":"Natural Resources for Human Health","volume":"47 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140082716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Asthma is a chronic allergic disease that affects the airways of the lungs. It makes breathing, speaking, and moving difficult or impossible. The current research aims to evaluate the ameliorative potential of stigmasterol-enriched extract (STIG) on ovalbumin (OVA)-induced asthma in a guinea pigs model. STIG was given orally to guinea pigs with asthma for a total of 28 days (dose of 100 mg/kg/b.w.). Guinea pigs exposed to OVA exhibited severe airway remodelling and inflammation resembling chronic asthma, as shown by the histological findings, elevated cytokines associated with inflammation, malondialdehyde (MDA) and reduced antioxidant enzymes. However, STIG significantly decreases inflammatory mediators and MDA levels and also enhances the antioxidant enzyme level. Furthermore, network pharmacology showed that STIG could combat asthma by acting on key targets and closely related pathways. Hence, it is concluded that STIG has the potential to reduce lung airway inflammation and oxidative stress, and it could serve as a promising medication for the management of asthma.
{"title":"Protective effect of stigmasterol enriched extract of Bacopa monnieri in ovalbumin (OVA)-induced asthma: An experimental and network pharmacology studies","authors":"Shiv Narayan, Sudeep Kumar Mandal, J. K. Gupta","doi":"10.53365/nrfhh/177625","DOIUrl":"https://doi.org/10.53365/nrfhh/177625","url":null,"abstract":"Asthma is a chronic allergic disease that affects the airways of the lungs. It makes breathing, speaking, and moving difficult or impossible. The current research aims to evaluate the ameliorative potential of stigmasterol-enriched extract (STIG) on ovalbumin (OVA)-induced asthma in a guinea pigs model. STIG was given orally to guinea pigs with asthma for a total of 28 days (dose of 100 mg/kg/b.w.). Guinea pigs exposed to OVA exhibited severe airway remodelling and inflammation resembling chronic asthma, as shown by the histological findings, elevated cytokines associated with inflammation, malondialdehyde (MDA) and reduced antioxidant enzymes. However, STIG significantly decreases inflammatory mediators and MDA levels and also enhances the antioxidant enzyme level. Furthermore, network pharmacology showed that STIG could combat asthma by acting on key targets and closely related pathways. Hence, it is concluded that STIG has the potential to reduce lung airway inflammation and oxidative stress, and it could serve as a promising medication for the management of asthma.","PeriodicalId":394827,"journal":{"name":"Natural Resources for Human Health","volume":"74 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139452578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Musa Isah, Gokhan Zengin, Wan Nor Amilah Wan Abdul Wahab, Hasmah Abdullah, Mohd Dasuki Sul’ain, Abdullahi Ibrahim Uba, Wan Rosli Wan Ishak, Shajarahtunnur Jamil
Melaleuca cajuputi plant has piqued the interest of researchers due to its pharmacological properties. However, there is scarce information regarding its enzyme inhibitory effects and possible mechanism of action. Thus, this study aimed to investigate the antioxidant, enzyme inhibitory, and potential binding interactions of bioactive components in M. cajuputi extract and fractions with the target enzymes. The 1,1-diphenyl-2-picrylhydrazyl radical scavenging (DPPH) assay was used for the antioxidant activity. The enzyme inhibition potential was assessed using tyrosinase and acetylcholinesterase (AChE) enzymes. M. cajuputi methanolic extract (MCME) demonstrated the most potent scavenging activity in comparison to Melaleuca fraction (MF1) and (MF2), as evidenced by IC50 values of 28.14 ± 0.96, 35.26 ± 0.64, and 80.90 ± 0.17 μg/mL, respectively. Similarly, the results of the enzyme inhibition assay showed that the MCME (IC50 65.81± 0.39 μg/mL) had the highest potency on tyrosinase enzyme compared to MF1 (IC50 131.3± 0.43 μg/mL) and MF2 (IC50 215.3 ± 0.48 μg/mL). However, MF2 (IC50 3.35 ± 0.37 μg/mL) showed a remarkable AChE enzyme inhibition than MCME and MF1. On the other hand, MCME possessed a low toxicity effect with an LC50 of 781 ± 0.07 μg/mL, whereas MF1 and MF2 were non-toxic. The 10-methylanthracene-9-carboxaldehyde, 2-isopropyl-10-methylphenanthrene, 2-tert butylanthracene, β-eudesmol, and α-eudesmol showed a strong binding propensity ranging from -5.1 to -6.2 and -6.4 to -7.6 kcal/mol towards tyrosinase and AChE respectively. The leaf extract of M. cajuputi could be considered a novel source of naturally occurring antioxidants and promising enzyme inhibitors.
{"title":"Antioxidant, enzyme inhibition, toxicity, and molecular docking analysis of Melaleuca cajuputi leaf extract and fractions","authors":"Musa Isah, Gokhan Zengin, Wan Nor Amilah Wan Abdul Wahab, Hasmah Abdullah, Mohd Dasuki Sul’ain, Abdullahi Ibrahim Uba, Wan Rosli Wan Ishak, Shajarahtunnur Jamil","doi":"10.53365/nrfhh/176775","DOIUrl":"https://doi.org/10.53365/nrfhh/176775","url":null,"abstract":"Melaleuca cajuputi plant has piqued the interest of researchers due to its pharmacological properties. However, there is scarce information regarding its enzyme inhibitory effects and possible mechanism of action. Thus, this study aimed to investigate the antioxidant, enzyme inhibitory, and potential binding interactions of bioactive components in M. cajuputi extract and fractions with the target enzymes. The 1,1-diphenyl-2-picrylhydrazyl radical scavenging (DPPH) assay was used for the antioxidant activity. The enzyme inhibition potential was assessed using tyrosinase and acetylcholinesterase (AChE) enzymes. M. cajuputi methanolic extract (MCME) demonstrated the most potent scavenging activity in comparison to Melaleuca fraction (MF1) and (MF2), as evidenced by IC50 values of 28.14 ± 0.96, 35.26 ± 0.64, and 80.90 ± 0.17 μg/mL, respectively. Similarly, the results of the enzyme inhibition assay showed that the MCME (IC50 65.81± 0.39 μg/mL) had the highest potency on tyrosinase enzyme compared to MF1 (IC50 131.3± 0.43 μg/mL) and MF2 (IC50 215.3 ± 0.48 μg/mL). However, MF2 (IC50 3.35 ± 0.37 μg/mL) showed a remarkable AChE enzyme inhibition than MCME and MF1. On the other hand, MCME possessed a low toxicity effect with an LC50 of 781 ± 0.07 μg/mL, whereas MF1 and MF2 were non-toxic. The 10-methylanthracene-9-carboxaldehyde, 2-isopropyl-10-methylphenanthrene, 2-tert butylanthracene, β-eudesmol, and α-eudesmol showed a strong binding propensity ranging from -5.1 to -6.2 and -6.4 to -7.6 kcal/mol towards tyrosinase and AChE respectively. The leaf extract of M. cajuputi could be considered a novel source of naturally occurring antioxidants and promising enzyme inhibitors.","PeriodicalId":394827,"journal":{"name":"Natural Resources for Human Health","volume":"21 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139000735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. O. Owheruo, G. Edo, Patrick Othuke Akpoghelie, K. A. Okoronkwo, Morgan Ugochukwu Nwaogu, Aghoghoroghene Elizabeth Ojulari, Joy Johnson Agbo
This work focuses on extracting oil from five local seeds using n-hexane in a Soxhlet apparatus. This study was designed to establish the suitability of the oils for domestic and industrial uses. Before extracting the oil, the nearest part of the seeds was inspected. Oils were extracted from selected underutilized seeds such as cashew (CS), orange seed (ORS), watermelon seed (WMS), paw-paw seed (PWS) and cherry seed (CHS) using n-hexane. The oil was analyzed for colour; with conventional techniques, calculate the saponification value, iodine value, acid value, percentage of unsaponifiable matter, and heat of combustion. The findings for proximal structure showed that fat ranged from 7.09 to 60.68% and protein from 17.97 to 31.16%. The levels of acidity, peroxide, iodine, saponification, unsaponifiable materials, and Polenske and density ranged from 1.07 to 5.94 mg KOH/g, 1.89 to 4.00 Meq/kg, 93.18 to 275.55 (MgI2/g), 68.41 to 326.16 KOH/g, 2.57 to 7.22 %, 1.37 to 7.44 and 0.74 to 0.87 g/L respectively. Smoke, flash, and fire points range from 75 to 90 0C, 80 to 170 0C and 230 to 300 0C respectively. According to the fatty acid composition, oleic and linoleic acid ranges were 17.14 and 60.63%, respectively. These criteria' values fell within the FAO's recommended range for edible oils. The samples used in the research oils are of high quality and suggested for use in industrial and culinary applications.
{"title":"Physicochemical Characteristics of Oils Extracted from Selected Underutilized Seeds in Ozoro, Delta State, Nigeria","authors":"J. O. Owheruo, G. Edo, Patrick Othuke Akpoghelie, K. A. Okoronkwo, Morgan Ugochukwu Nwaogu, Aghoghoroghene Elizabeth Ojulari, Joy Johnson Agbo","doi":"10.53365/nrfhh/176713","DOIUrl":"https://doi.org/10.53365/nrfhh/176713","url":null,"abstract":"This work focuses on extracting oil from five local seeds using n-hexane in a Soxhlet apparatus. This study was designed to establish the suitability of the oils for domestic and industrial uses. Before extracting the oil, the nearest part of the seeds was inspected. Oils were extracted from selected underutilized seeds such as cashew (CS), orange seed (ORS), watermelon seed (WMS), paw-paw seed (PWS) and cherry seed (CHS) using n-hexane. The oil was analyzed for colour; with conventional techniques, calculate the saponification value, iodine value, acid value, percentage of unsaponifiable matter, and heat of combustion. The findings for proximal structure showed that fat ranged from 7.09 to 60.68% and protein from 17.97 to 31.16%. The levels of acidity, peroxide, iodine, saponification, unsaponifiable materials, and Polenske and density ranged from 1.07 to 5.94 mg KOH/g, 1.89 to 4.00 Meq/kg, 93.18 to 275.55 (MgI2/g), 68.41 to 326.16 KOH/g, 2.57 to 7.22 %, 1.37 to 7.44 and 0.74 to 0.87 g/L respectively. Smoke, flash, and fire points range from 75 to 90 0C, 80 to 170 0C and 230 to 300 0C respectively. According to the fatty acid composition, oleic and linoleic acid ranges were 17.14 and 60.63%, respectively. These criteria' values fell within the FAO's recommended range for edible oils. The samples used in the research oils are of high quality and suggested for use in industrial and culinary applications.","PeriodicalId":394827,"journal":{"name":"Natural Resources for Human Health","volume":"25 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139000756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anubhav Singh, M. S. Sankhla, Vaibhav Sharma, Poonam Kumari, Abhishek R. Rai, Archana Gautam, Tina Sharma, K. Awasthi, Rajeev Kumar
Extensive research has explored the potential utility of industrial hemp (Cannabis sativa) in phytoremediation, particularly its ability to alleviate soil pollution caused by heavy metals—a significant threat to human health and the environment. Various industrial activities, including mining, smelting, and manufacturing, can introduce heavy metals into the soil. Phytoremediation harnesses the unique detoxification abilities of plants like cannabis to rehabilitate polluted sites. Studies indicate that cannabis exhibits remarkable proficiency in extracting heavy metals like lead, cadmium, and nickel from contaminated soil, accumulating them in its tissues. This highlights the promising potential of cannabis as a candidate for phytoremediation. Noteworthy attributes such as hemp's deep roots and robustness enable it to endure diverse metal accumulations, and its biomass presents numerous commercial opportunities post-harvest. Additionally, research suggests that the integration of mycorrhizal fungi may enhance cannabis' phytoremediation capabilities. These symbiotic fungi establish beneficial relationships with plants, promoting nutrient absorption and fortifying environmental resilience.
{"title":"Exploring the Potential of Industrial Hemp in Phytoremediation of Heavy Metals","authors":"Anubhav Singh, M. S. Sankhla, Vaibhav Sharma, Poonam Kumari, Abhishek R. Rai, Archana Gautam, Tina Sharma, K. Awasthi, Rajeev Kumar","doi":"10.53365/nrfhh/176819","DOIUrl":"https://doi.org/10.53365/nrfhh/176819","url":null,"abstract":"Extensive research has explored the potential utility of industrial hemp (Cannabis sativa) in phytoremediation, particularly its ability to alleviate soil pollution caused by heavy metals—a significant threat to human health and the environment. Various industrial activities, including mining, smelting, and manufacturing, can introduce heavy metals into the soil. Phytoremediation harnesses the unique detoxification abilities of plants like cannabis to rehabilitate polluted sites. Studies indicate that cannabis exhibits remarkable proficiency in extracting heavy metals like lead, cadmium, and nickel from contaminated soil, accumulating them in its tissues. This highlights the promising potential of cannabis as a candidate for phytoremediation. Noteworthy attributes such as hemp's deep roots and robustness enable it to endure diverse metal accumulations, and its biomass presents numerous commercial opportunities post-harvest. Additionally, research suggests that the integration of mycorrhizal fungi may enhance cannabis' phytoremediation capabilities. These symbiotic fungi establish beneficial relationships with plants, promoting nutrient absorption and fortifying environmental resilience.","PeriodicalId":394827,"journal":{"name":"Natural Resources for Human Health","volume":"126 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138998377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：