THE COMBINATION OF HIGH FAT DIET AND MONOSODIUM GLUTAMATE ALTERING ADIPOGENESIS, BRAIN RESISTIN AND SERUM CORTISOL LEVEL IN FEMALE RAT

Home / Archives / Vol. 8 No. 2 (2022): July / Research Article THE COMBINATION OF HIGH FAT DIET AND MONOSODIUM GLUTAMATE ALTERING ADIPOGENESIS, BRAIN RESISTIN AND SERUM CORTISOL LEVEL IN FEMALE RAT Authors Aris Widayati Department of Physiology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia Dewi Mustika Department of Physiology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia Yhusi Karina Riskawati Department of Physiology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia Agustin Iskandar Department of Parasitology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia Nia Kurnianingsih Department of Physiology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia DOI: https://doi.org/10.21776/ub.mnj.2022.008.02.8 Keywords: glutamate, fat diet, inflammation, behavior, BDNF Abstract Background: The prevalence of obesity is increasing tremendously worldwide. Obesity is correlated with the consumption of a high-fat diet (HFD) and monosodium glutamate (MSG). Although the effect of MSG or HFD on inflammation has been established, lack of information about the effects of both combination on simultaneous systemic and brain inflammation that can be evaluated from cortisol serum and brain resistin level respectively. Objectives: This study aimed to investigate the effects of combining HFD and MSG on brain resistin, serum cortisol, lipid profiles, visceral weight, and body weight gain. Methods: Sixteen adult female rats were randomized into 4 groups consist of standard diet; HFD+MSG 0.05 mg/gBW, HFD+MSG 0.2 mg/gBW and HFD+MSG 0.35 mg/gBW. Lipid profiles were measured using automatic biochemical analyzer. Brain resistin and serum cortisol level were determined using enzyme-linked immunoassay. Results: The groups of HFD+MSG significantly have higher level of brain resistin (p<0.003), serum cortisol (p<0.01), total cholesterol (p<0.003) and visceral fat weight (p<0.05) than control group. Conclusion: The combination of HFD, and MSG generates obesity that led to systemic-brain alteration on inflammation. Further study is necessary to evaluate further the functional impact of both combinations on behavioral profiles.