大肠杆菌csgD基因编码生物膜形成的主要调控:多个转录因子之间的相互作用

H. Ogasawara, A. Kori, Kayoko Yamada, Kaneyoshi Yamamoto, A. Ishihama
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引用次数: 2

摘要

在自然界的应激条件下,大肠杆菌形成生物膜以长期生存。卷毛是生物膜内细胞接触的基本结构。curli的结构成分和组装因子由csgBA和csgDEFG两个不同排列的操纵子编码,并受csgD基因产物调控。因此,CsgD是细菌生物膜形成的主要调节因子。csgD启动子受多种转录因子控制,包括CRP、CpxR、Crl、H-NS、IHF、MlrA、OmpR、RcsB和RstA,反映了生物膜形成对各种外部因素和条件的响应。我们对这几种转录因子对csgD启动子的调控机制进行了系统分析。在体外以csgD启动子的不同片段作为DNA探针,对这些因子的不同组合进行DNA结合实验后,我们不仅发现了正调控因子和负调控因子之间的竞争,还发现了正调控因子和负调控因子之间的合作相互作用。这些发现提出了一个调控模型,其中csgD启动子是由这些转录因子的功能分子的相对水平控制的。
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Regulation of the E. coli csgD gene encoding the master regulator of biofilm formation: Interplay between multiple transcription factors
Under stressful conditions in nature, Escherichia coli forms biofilms for long-term survival. Curli fimbriae are an essential architecture for cell-cell contacts within biofilms. Structural components and assembly factors of curli are encoded by two divergently arranged operons, csgBA and csgDEFG, which are regulated by the csgD gene product. Thus, CsgD is a master regulator of bacterial biofilm formation. Reflecting the response of biofilm formation to various external factors and conditions, the csgD promoter is under the control of multiple transcription factors including CRP, CpxR, Crl, H-NS, IHF, MlrA, OmpR, RcsB and RstA. We have conducted a systematic analysis of the regulation mechanism of the csgD promoter by these multiple transcription factors. After the DNA binding assays in vitro of various combinations of these factors with various segments of the csgD promoter as DNA probes, we realized not only competition between positive and negative regulators but also cooperative interplay within both positive and negative factor groups. These findings raise a regulation model, in which the csgD promoter is controlled by the relative levels of functional molecules of these transcription factors.
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