S. Hejazi, S. Saberi, R. Arjmand, S. Soleimanifard
{"title":"p -糖蛋白A、g -谷氨酰半胱氨酸合成酶1和甘油oporin 1基因在未愈合的人畜共患皮肤利什曼病中的表达研究","authors":"S. Hejazi, S. Saberi, R. Arjmand, S. Soleimanifard","doi":"10.34172/jsums.2021.27","DOIUrl":null,"url":null,"abstract":"Background and aims: Regarding the increasing numbers of clinical antimonial-resistant Leishmaniasis, understanding the reasons for drug resistance is helpful. This study aimed to find the expression level of the genes related to resistance, P-glycoprotein A (PgpA), G-glutamylcysteine synthetase 1 (Gsh1), and aquaglyceroporin 1 (Aqp1) in antimonial-resistant clinical isolates. Methods: Samples were isolated from leishmaniasis ulcers of 10 non-healing patients and the species were identified by the nested-polymerase chain reaction (PCR) method. In vitro experiments were performed using the amastigote-macrophage model by J774 cell line, and in vivo studies were conducted by animal model, the Balb/c mice. Finally, the values of genes expression were determined by quantitative-reverse transcription (q-RT) real-time PCR method and then compared with non-resistant Leishmania major (MRHO/IR/75/ER). Results: Molecular identification showed that all isolated protozoa were L. major. The isolated samples from clinical resistant patients represented no increase in expression in the tested resistance genes (P>0.05). Finally, it was found that the lack of improvements in patients was not associated with the increased expression of resistance genes. Conclusion: In general, no inherent resistance was observed in the tested samples neither a correlation between the healing of lesions and the level of genes expression.","PeriodicalId":318974,"journal":{"name":"Journal of Shahrekord University of Medical Sciences","volume":"42 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2021-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The study of P-glycoprotein A, G-glutamylcysteine synthetase 1, and aquaglyceroporin 1 genes expression in non-healing zoonotic cutaneous leishmaniasis cases\",\"authors\":\"S. Hejazi, S. Saberi, R. Arjmand, S. Soleimanifard\",\"doi\":\"10.34172/jsums.2021.27\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background and aims: Regarding the increasing numbers of clinical antimonial-resistant Leishmaniasis, understanding the reasons for drug resistance is helpful. This study aimed to find the expression level of the genes related to resistance, P-glycoprotein A (PgpA), G-glutamylcysteine synthetase 1 (Gsh1), and aquaglyceroporin 1 (Aqp1) in antimonial-resistant clinical isolates. Methods: Samples were isolated from leishmaniasis ulcers of 10 non-healing patients and the species were identified by the nested-polymerase chain reaction (PCR) method. In vitro experiments were performed using the amastigote-macrophage model by J774 cell line, and in vivo studies were conducted by animal model, the Balb/c mice. Finally, the values of genes expression were determined by quantitative-reverse transcription (q-RT) real-time PCR method and then compared with non-resistant Leishmania major (MRHO/IR/75/ER). Results: Molecular identification showed that all isolated protozoa were L. major. The isolated samples from clinical resistant patients represented no increase in expression in the tested resistance genes (P>0.05). Finally, it was found that the lack of improvements in patients was not associated with the increased expression of resistance genes. Conclusion: In general, no inherent resistance was observed in the tested samples neither a correlation between the healing of lesions and the level of genes expression.\",\"PeriodicalId\":318974,\"journal\":{\"name\":\"Journal of Shahrekord University of Medical Sciences\",\"volume\":\"42 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-12-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Shahrekord University of Medical Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.34172/jsums.2021.27\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Shahrekord University of Medical Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.34172/jsums.2021.27","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The study of P-glycoprotein A, G-glutamylcysteine synthetase 1, and aquaglyceroporin 1 genes expression in non-healing zoonotic cutaneous leishmaniasis cases
Background and aims: Regarding the increasing numbers of clinical antimonial-resistant Leishmaniasis, understanding the reasons for drug resistance is helpful. This study aimed to find the expression level of the genes related to resistance, P-glycoprotein A (PgpA), G-glutamylcysteine synthetase 1 (Gsh1), and aquaglyceroporin 1 (Aqp1) in antimonial-resistant clinical isolates. Methods: Samples were isolated from leishmaniasis ulcers of 10 non-healing patients and the species were identified by the nested-polymerase chain reaction (PCR) method. In vitro experiments were performed using the amastigote-macrophage model by J774 cell line, and in vivo studies were conducted by animal model, the Balb/c mice. Finally, the values of genes expression were determined by quantitative-reverse transcription (q-RT) real-time PCR method and then compared with non-resistant Leishmania major (MRHO/IR/75/ER). Results: Molecular identification showed that all isolated protozoa were L. major. The isolated samples from clinical resistant patients represented no increase in expression in the tested resistance genes (P>0.05). Finally, it was found that the lack of improvements in patients was not associated with the increased expression of resistance genes. Conclusion: In general, no inherent resistance was observed in the tested samples neither a correlation between the healing of lesions and the level of genes expression.