Ujang Siron, Noertjahyani Noertjahyani, Yana Taryana, Romiyadi Romiyadi
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引用次数: 2

摘要

本文旨在研究NAA和BAP浓度对石斛原球茎生长的互作效应,了解石斛原球茎生长的适宜浓度。本研究方法采用在Sumedang区Tanjungsari街道Winaya Mukti大学农学院实验室进行的实验方法。该实验于2017年6月至2017年9月进行。试验采用完全随机设计(CRD),因子模式,包括两个因素,重复两次。第一个因素是NAA浓度的影响,NAA浓度分为不含NAA、0.5 mg kg-1、1.0 mg kg-1、1.5 mg kg-1和2.0 mg kg-1 5个水平。第二个因素是BAP浓度,由5个级别组成,即不含BAP, 1.0 mg kg-1, 2.0 mg kg-1, 3.0 mg kg-1和4.0 mg kg-1。Eksplant是兰花D. spectabile的原球茎,在MS培养基(Murashig和Skoog)一半配方上作为基础培养基,每次处理12周。试验结果表明,NAA和BAP浓度仅对叶片数有交互作用。无NAA或NAA浓度为1.5 mg kg-1, BAP浓度为2.0 mg kg-1,叶片多。NAA和BAP浓度不影响植株的芽数、根数、根长、鲜重和干重,也不影响植株的生长能力。BAP浓度仅影响株高,未添加BAP的培养基株高最高
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Pengaruh Konsentrasi Zat Pengatur Tumbuh Naphthalene Acetic Acid dan Benzil Amino Purin terhadap Pertumbuhan Protokorm Anggrek Dendrobium spectabile pada Kultur In Vitro
The aim of the article is to study the interaction effect between NAA and BAP concentration on protocorm growth and to know the proper concentration for growth of Dendrobium  spectabile  orchid protocorm. This research method using an experimental method which is conducted in the Laboratory of Agriculture Faculty of Winaya Mukti University, Tanjungsari Subdistrict, Sumedang District. The experiment was conducted from June 2017 until September 2017. The experiment used was a Completely Randomized Design (CRD) with the factorial pattern, consisting of two factors and repeated twice. the first factor was the effect of NAA concentration which consisted of five levels, namely without NAA, 0.5 mg kg-1, 1.0 mg kg-1, 1.5 mg kg-1, and 2.0 mg kg-1.  The second factor is the BAP concentration which consists of five levels, namel without BAP, 1.0 mg kg-1, 2.0 mg kg-1, 3.0 mg kg-1, and 4.0 mg kg-1. Eksplant is protocorm from orchid D. spectabile which is grown on MS medium (Murashig and Skoog) half recipe as base medium accompanied by each treatment for 12 weeks. The experimental results show that there is an interaction between the effect of NAA and BAP concentration on the number of leaves only. Without NAA or 1.5 mg kg-1 NAA concentration with BAP 2.0 mg kg-1 gives more leaves.  Independent of NAA or BAP concentrations did not affect the number of buds, number of roots, root length, fresh and dry weight of plantlets, and also growth ability of plantlets.  BAP concentration only affect plant height, and the highest plantlet height is found without add of BAP in medium culture 
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