Isolation and Determination of Amylase Enzyme Activity from Durian Seed Sprouts

: One of the favorite fruits in Medan-Indonesia is durian. The number of fruit is comparable to the most waste of durian seed produced. Seeds are one of the ways to breed through the process of germination. In the process of germination which plays an important role is the enzyme amylase. Based on this, the research aims to isolate the enzyme amylase found in durian seed sprouts on the fifth day through dialysis with ammonium sulfate and then determined its activity against temperature, pH, substrate concentration. Through this research, the enzyme amylase has been isolated from the sprouts of durian beans with the best activity at temperature 40 oC (36.87 U/ml), pH 7 (36.86 U/ml), substrate concentration 1% (26.36 U/ml). The best ammonium sulfate saturation in the dialysis process is 60% acquired enzyme activity of 58.59 U/ml.