淡紫色紫毛霉11BB株防治根结线虫的潜力评价

P. V. Nguyễn, H. Mai, Tuyen T. T. Doan, Thanh T. L. Bien
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摘要

寄生真菌被认为是防治植物寄生线虫的有效生物制剂。根据形态和ITS序列分析,从144份土壤样品中分离到7株紫丁香紫毛菌。这些土壤样品分别采集于巴黎头省周德区和同奈省定泉区。在添加酪蛋白、几丁质和吐温20的培养基中培养96 h后,7株菌株显示出蛋白酶、几丁质酶和脂肪酶活性。在实验室条件下,11BB和11SN菌株的虫卵寄生率分别为31 ~ 34%和58 ~ 62%。在温室条件下,与对照相比,11BB菌株在番茄植株上的幼虫数减少79.0 ~ 80.3%,卵数减少77.4 ~ 79.7%。结果表明,该菌株可用于根结线虫的防治。
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Evaluation of the control potential to root - knot nematode Meloidogyne incognita of Purpureocillium lilacinum 11BB strain
Parasitic fungi are considered effective biological agents to control plant -parasitic nematodes. Based on morphology and ITS sequence, seven Purpureocillium lilacinum strains were isolated from 144 soil samples collected from Chau Duc district, Ba Ria - Vung Tau province, and Dinh Quan district, Dong Nai province. After 96 h of culture in medium supplemented with casein, chitin, and tween 20, seven strains showed protease, chitinase, and lipase activity. In laboratory conditions, both 11BB and 11SN strains parasitzed 31 – 34% eggs and 58 – 62% eggmass, respectively. In greenhouse conditions, the 11BB strain decreased 79.0 - 80.3% of juveniles (J2s) and 77.4 - 79.7% of egg numbers on tomato plants as compared with the control. Results showed that the 11BB fungal strain could be used in control of root - knot nematodes.
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