用玻尔染色组织学标本确定创伤性和非创伤性出血形成时间的不同可能性

M. Garazdiuk
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摘要

对创伤性(HTG)和非创伤性(HNG)脑出血(HB)形成的确切时间进行验证,可以在危害人类生命的犯罪案件中大大缩小嫌疑人范围。工作的目的。人脑组织切片光镜下创伤性和非创伤性出血形成年龄的法医诊断标准的建立。材料和方法。本研究采用80例已知出血时间为2 ~ 15天的尸体的脑组织染色标本,根据医学文献:HNG - 30例死亡的脑组织标本(1组),HTG - 30例死亡的脑组织标本(2组),冠心病死亡的脑组织标本20例(3组)。分析现有的组织修复,发现出血年龄的相应时间依赖性不是线性的,相反,其值有波动——不同原因的出血形成后,HB色素呈现不均匀,时间逐渐增加。用珍珠染色法对脑物质的微制剂进行组织学检查,对确定出血的年龄无效。
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DIFFERENTIAL POSSIBILITIES OF THE METHOD OF STAINING HISTOLOGICAL SPECIMENTS BY PERLS FOR ESTABLISHING THE TIME OF FORMATION OF TRAUMATIC AND NONTRAUMATIC GENESIS HEMORRHAGES
Verifi cation of the exact time of formation of hemorrhages in the human brain (HB) of traumatic (HTG) and non-traumatic (HNG) genesis makes it possible to signifi cantly narrow the circle of suspects in the case of a crime against human life.Aim of the work. Developing of forensic criteria for the diagnosis of the age of formation of hemorrhages of traumatic and non-traumatic genesis by light microscopy of histological sections of human brain.Materials and methods. For the study used stained histological specimens of the brain from 80 corpses with a known time of hemorrhage from 2 to 15 days, according to medical documents: in case of death from HNG – 30 histological specimens (1 group), death from HTG – 30 histological samples (group 2), brain preparations in case of death from coronary heart disease were selected for control – 20 samples (group 3).Results. Analyzing the available histopreparations, it was found that the corresponding time dependences of the age of hemorrhage are not linear, but on the contrary have fluctuations in their values – uneven appearance of pigment in HB with a gradual increase in time since the formation of hemorrhages of diff erent genesis.Conclusion. Histological examination of micropreparations of the substance of the brain, stained by the method of Pearls, is not eff ective for determining the age of hemorrhage.
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