肿瘤细胞系中微丝网络的激波相互作用

S. Hosseini, S. Moosavi-Nejad, K. Takayama
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摘要

冲击波与肾肿瘤细胞系(ACHN)的相互作用研究了其膜微丝结构作为抵抗机械应力的主要细胞系统。在覆盖玻片上培养ACHN单层,用峰值压力为16 MPa的水下冲击波处理。形态学变形被发现与细胞内细胞骨架细丝的紊乱有关。为了模拟激波与细胞纤维网状结构的相互作用,将浸没在水中的多孔薄层棉花暴露在水下激波中。用针形水听器和光纤探头水听器测量了冲击超压衰减。对激波运动进行了定量可视化。将实验结果扩展到了解在ESW癌症治疗过程中可能发生的休克/细胞相互作用的复杂过程
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Shock Wave Interaction with Micro-Filament Network in a Cancer Ce1l line
Interaction of shock waves with renal tumor cell line (ACHN) has been studied with regard to their membrane micro-filament structure as a major cellular system to resist mechanical stress. ACHN monolayer cultured on a cover slide glass was treated with 16 MPa peak pressure focused underwater shock waves. The morphological deformations were found to be associated with disorganization of the intracellular cytoskeletal filaments. In order to simulate the interaction of shock waves with fibrillar network structure of cells, thin porous layers of cotton immersed in water were exposed to underwater shock waves. Attenuation of shock over-pressures were measured with needle and fiber optic probe hydrophones. The motion of shock waves was quantitatively visualized. The experimental results were extended to understand the complex process of shock/cell interactions, which would happen during ESW cancer therapy
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