Cheng-Yeh Huang, M. Chiang, S. Fan, A. Ghaemmaghami, W. Hsu
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Label-free separation and sorting of human monocytes and T-cells by electrowetting and dielectrophoresis
We report a label-free separation and sorting approach based on electrowetting-on-dielectric (EWOD) and dielectrophoresis (DEP) to manipulate human monocyte (THP-1) and T-cell (Jurkat) lines in droplets without any impact on cell viability. In addition to separating cells, this device provides a platform to study cell-cell interactions and migration before the separation.
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