科尼亚美利奴母羊单羔和双羔产后血浆妊娠相关糖蛋白谱

IF 0.2 4区 农林科学 Q4 VETERINARY SCIENCES Revista Cientifica-Facultad De Ciencias Veterinarias Pub Date : 2023-09-26 DOI:10.52973/rcfcv-e33298
Eyyup Hakan Ucar, Mehmet Kose, Mesut Kirbas, Faruk Bozkaya, Mehmet Osman Atli
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引用次数: 0

摘要

本研究采用专用的牛妊娠试验试剂盒对科尼亚美利奴母羊产羔后血浆妊娠相关糖蛋白(妊娠相关糖蛋白)进行了检测。以16头科尼亚美利奴母羊为材料。将母羊分为4组,分别产下1只公羊羔(SM组,n=4)、1只母羊羔(SF组,n=4)、2只公羊羔(TM组,n=4)和2只母羊羔(TF组,n=4)。在第0天(产羔日)、第3天、第7天、第10天、第14天采集血浆,然后每周采集一次,直至第35天。所有样品均采用牛妊娠试验试剂盒检测PAGs水平。结果表明,仔猪外周血PAGs水平与产羔后天数呈极显著负相关(r2=0.969;术中,0.01)。但PAGs水平与羔羊性别、出生类型无显著关系。综上所述,无论羔羊性别和出生类型,科尼亚美利奴母羊血浆PAGs水平均迅速下降。此外,结果表明,基于牛elisa的商业妊娠试验试剂盒可以监测科尼亚美利奴母羊产羔后血浆中PAG的分布,检测结果可用于基于PAG分子水平的决策和评估。
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Plasma profile of Pregnancy Associated Glycoproteins during Postpartum period in Konya Merino ewes lambing single and twin
This study investigated the plasma profile of Pregnancy Associated Glycoproteins (PAGs) after lambing in Konya Merino ewes using specific cattle pregnancy test kit. A total of 16 Konya Merino ewes were used as a material. Four groups were set up, ewes birthing a male lamb (SM group, n=4), a female lamb (SF group, n=4), twin male lambs (TM group, n=4) or twin female lambs (TF group, n=4). Blood plasma samples were taken on days 0 (lambing day), 3, 7, 10, 14 and then weekly until day 35. All samples were analysed using a bovine pregnancy test kit to detect of PAGs level. It was determined that there was a strong negative correlation between PAGs levels in peripheral blood and days after lambing (r2=0.969; P<0.01). However, no relationship was found between PAGs level and lamb gender or birth type. In conclusion, plasma PAGs level decreases rapidly in Konya Merino ewes regardless of lamb gender and birth type. Moreover, the results showed that the plasma profile of PAGs after lambing in Konya Merino ewes can be monitored with a bovine commercial ELISA–based pregnancy test kit, and the test results can be used in decisions and assessments based on the levels of PAG molecules.
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CiteScore
0.20
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0.00%
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66
审稿时长
18-36 weeks
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