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Survival of a mixed culture of microencapsulated probiotic strains against the gastrointestinal barrier in vitro 微囊化益生菌菌株混合培养对胃肠道屏障的体外存活
4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-10-13 DOI: 10.52973/rcfcv-e33296
Luz Alba Caballero–Pérez, Rene Tejedor–Arias, Elaysa Josefina Salas–Osorio
Encapsulating materials preserve the viability of probiotics under gastrointestinal conditions. The aim of the research was to evaluate the protective effect of an encapsulating matrix, composed for the first time with three prebiotic materials to maintain the viability of a mixed culture of spray–dried microencapsulated probiotics under simulated gastrointestinal and prebiotic conditions. Microcapsules of four formulations with better viability were then evaluated by inoculating microencapsulated and free strains in MRS broth, adjusting three pH values, bile salts, broth with and without carbohydrate (prebiotic test), incubated at 36 ± 1°C / 24 h; then the percentage of post–treatment cell survival was calculated. Showing that, formulation 1 presented higher barrier protection with average counts: 7.31 log CFU·g-1 lactobacilli and 7.75 log CFU·g-1 (Saccharomyces boulardii) / 4 h (SGF), reaching 6.78 log CFU·g-1 in the four formulations (SIF) with a higher average survival rate 79.79% and 85.06% SGF and SIF, in vitro. On the other hand, the prebiotic test maintained average counts of 9.40 log CFU·g-1 (Lactobacillus spp.) and 6.99 log CFU·g-1 (S. boulardii) / 24 h. The protection exerted by the microspheres under simulated gastrointestinal and prebiotic conditions at therapeutic levels (≥ 106 CFU·mL-1) was demonstrated.
包封材料可以在胃肠道条件下保持益生菌的活力。本研究的目的是评估首次由三种益生元材料组成的包封基质在模拟胃肠道和益生元条件下维持喷雾干燥微胶囊益生菌混合培养活力的保护作用。将微囊化菌和游离菌分别接种于MRS培养液中,调整3种pH值、胆汁盐、加糖和不加糖的培养液(益生元试验),在36±1℃/ 24 h下培养,以评价4种活性较好的配方微胶囊;然后计算处理后细胞存活率。结果表明,配方1具有较高的屏障保护作用,平均乳酸菌数量分别为7.31 log CFU·g-1和7.75 log CFU·g-1(博氏酵母菌)/ 4 h (SGF), 4种配方(SIF)的体外平均存活率分别为79.79%和85.06%,达到6.78 log CFU·g-1。另一方面,益生元试验维持了9.40 log CFU·g-1(乳酸杆菌)和6.99 log CFU·g-1(博氏沙门氏菌)/ 24 h的平均计数。在模拟胃肠道和益生元条件下(≥106 CFU·mL-1),微球的保护作用得到了证实。
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引用次数: 0
The effect of myo–inositol supplementation on feed physicochemical structure and viral load of dry cat food contaminated with SARS–CoV–2 by simulating sneezing 模拟打喷嚏法研究添加肌醇对SARS-CoV-2污染干猫粮饲料理化结构和病毒载量的影响
4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-10-13 DOI: 10.52973/rcfcv-e33282
Serol Korkmaz, Ayşe Parmaksız, Burcu Irem Omurtag–Korkmaz, Ahmet Sait
The study was carried to investigate the effect of myo–inositol supplementation on feed physicochemical structure and viral load of dry cat food contaminated with inactive SARS–CoV–2 by simulating sneezing. The most natural infection of severe acute respiratory syndrome coronavirus 2 (SARS–CoV–2) in animals is related to close contact with their owners with COVID–19 which is handling, taking care and feeding them. SARS–CoV–2 can survive on food, fomites and surfaces for extended periods related to environmental conditions. Many natural feed additives and supplements have been a candidate in recent antiviral treatment strategies against COVID–19. In this study, myo–inositol which is permitted in animal nutrition was used at different concentrations (0, 12.5, 25 and 50 mg·100 g-1 cat food) and conditions (22°C at room temperature and 4°C in the refrigerator) to investigate its effects on feed physicochemical structure and viral load of dry cat food contaminated with inactive SARS–CoV–2 by simulating sneezing. For the interactions between myo–inositol, feed structure and viral load, dry matter, moisture, water absorption index (WAI), water solubility index (WSI), pH and virus gene copy (GC) by RT–qPCR were measured. As only storage temperature affected both WAI and WSI as expected, myo–inostol supplementation dose–dependently decreased gene copy in dry cat food (IC50:366.4–581.5 mg·100 g-1 cat food) at 22°C storage temperature. Virus GC did not correlate with the dry matter, moisture content, pH and WAI after the 30 min contact time (except WSI). In conclusion, myo–inositol as a feed additive might have the potential to control serious viral infections such as COVID–19 for human–animal interactions in a One–Health context.
通过模拟打喷嚏的方法,研究添加肌醇对非活性SARS-CoV-2污染干猫粮饲料理化结构和病毒载量的影响。动物中最自然的严重急性呼吸综合征冠状病毒2 (SARS-CoV-2)感染与与感染COVID-19的主人密切接触有关,这些主人正在处理、照顾和喂养它们。SARS-CoV-2可以在与环境条件相关的食物、污染物和表面上存活较长时间。在最近针对COVID-19的抗病毒治疗策略中,许多天然饲料添加剂和补充剂已成为候选方案。本研究以动物营养中允许使用的肌醇为研究对象,在不同浓度(0、12.5、25和50 mg·100 g-1猫粮)和条件(室温22°C和冰箱4°C)下,通过模拟打喷嚏的方式,研究其对被非活性SARS-CoV-2污染的干猫粮饲料理化结构和病毒载量的影响。采用RT-qPCR法测定干物质、水分、吸水指数(WAI)、水溶性指数(WSI)、pH和病毒基因拷贝数(GC),研究肌醇、饲料结构与病毒载量的相互作用。正如预期的那样,只有储存温度会影响WAI和WSI,在22°C的储存温度下,肌醇补充剂量依赖性地减少了干猫粮(IC50:366.4-581.5 mg·100 g-1猫粮)的基因拷贝。接触30 min后,病毒GC与干物质、含水量、pH和WAI (WSI除外)均无相关性。总之,肌醇作为饲料添加剂可能有潜力控制严重的病毒感染,如COVID-19在单一健康环境下的人与动物相互作用。
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引用次数: 0
Análisis de la gestión técnico–económica de ganaderías ovinas de razas autóctonas de las Islas Baleares (España) 巴利阿里群岛本地品种牧羊场技术经济管理分析(西班牙)
4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-10-08 DOI: 10.52973/rcfcv-e33299
Yolanda Mena, Manuel Delgado–Pertiñez
Las Islas Baleares, España, poseen una importante tradición ganadera basada en el uso de razas autóctonas y en el pastoreo. Sin embargo, la intensificación de la producción primaria, la competencia por el territorio generado por el turismo y su baja rentabilidad, hacen que estos sistemas estén a punto de desaparecer, con negativas consecuencias ambientales y sociales. Para poder conservar estos sistemas, es necesario profundizar en el conocimiento del manejo, lo que permitirá mejorar su gestión técnica y económica y establecer estrategias que optimicen su rentabilidad, pero conservando sus beneficios socio–ambientales. Se seleccionaron 10 unidades productivas ovinas de dos razas autóctonas de Mallorca (Mallorquina y Roja Mallorquina) que fueron monitorizadas mensualmente durante 2015, con el objetivo de evaluar la gestión mediante la elaboración de indicadores técnico–económicos. Posteriormente, se realizó una evaluación de las debilidades del manejo, para detectar aspectos clave que ayuden a mejorar la rentabilidad de la actividad. Se observó que estas unidades productivas están poco especializadas y presentan diversos modelos productivos (profesional, recreativo, turístico, agrícola). Todas ellas están orientadas a la producción de corderos, menos una que es mixta, produciendo tres tipos diferentes de corderos según su peso (lechal, recental medio y grande). La autonomía alimentaria de las unidades productivas es alta, sin embargo, poseen poca organización reproductiva y los costes de producción no se cubren por el precio del cordero. Así mismo, solo el 30 % de las unidades productivas genera beneficio empresarial. Como estrategias de mejora se considera importante la mejora de la producción de pastos; la mejora de la planificación reproductiva y de la genética de las razas; el producir corderos de buena conformación de la canal o el crear una marca de calidad que informe al consumidor de los beneficios ambientales de estos sistemas y de la calidad nutritiva–funcional que tienen estos corderos criados en pastoreo.
西班牙的巴利阿里群岛有着重要的畜牧业传统,其基础是使用本地品种和放牧。然而,初级生产的集约化、旅游业对土地的竞争以及它们的低盈利能力意味着这些系统即将消失,造成不利的环境和社会后果。为了保护这些系统,有必要加深管理知识,这将使改进其技术和经济管理,并制定战略,优化其盈利能力,同时保持其社会和环境效益。2015年,我们从马略卡岛的两个本地品种(Mallorquina和red Mallorquina)中选择了10个生产单位,每月对它们进行监测,目的是通过制定技术经济指标来评估管理。随后,对管理弱点进行了评估,以确定有助于提高活动盈利能力的关键方面。这些生产单位的专业化程度较低,有不同的生产模式(专业、娱乐、旅游、农业)。所有这些都是为了生产羊羔,除了一个是混合的,根据重量生产三种不同类型的羊羔(小羊羔、中羊羔和大羊羔)。生产单位的粮食自主权很高,但它们的生殖组织很少,生产成本无法由羔羊的价格支付。同样,只有30%的生产单位产生商业利润。提高牧草产量被认为是重要的改进策略;改进生殖计划和品种遗传学;生产具有良好胴体形态的羊羔,或创建一个质量标志,告知消费者这些系统的环境效益和这些在放牧中饲养的羊羔的营养功能质量。
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引用次数: 0
Changes in hematological status depending on reproductive stage of ewes naturally infected with internal parasites in North–Eastern Algeria 阿尔及利亚东北部自然感染体内寄生虫的母羊的血液学状况随繁殖阶段的变化
4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-09-29 DOI: 10.52973/rcfcv-e33308
Ahmed Hadef, Souad Righi, Abdelbasset Ghouar
In Algeria, sheep farming is still largely traditional, and ewes are subject to various pathologies, particularly parasitic diseases leading to reproductive failure and production losses. To assess the influence of internal parasites (helminths and protozoa) and their interaction with the different phases of reproduction and season on hematological parameters, fecal and blood samples (n = 89) were taken from ewes during the period from the end of gestation to 3 months after parturition, two weeks apart. Animals were reared under a traditional pasture–based farming system in a Region of Northeastern Algeria characterized by a Mediterranean climate. Parasitic coproscopy results showed the consistent presence of digestive strongyles, Strongyloides, Trichuris ovis, Moniezia spp., Fasciola hepatica, and Coccidia. Hematological parameters, mainly hematocrit (HCT), hemoglobin (HGB), and erythrocytes (RBC), showed lower levels in anemic ewes throughout the study period. These hematological effects became significantly prevalent in 91% of the animals (P<0.05) during the third month postpartum (p.p.). The use of a mixed–effects model confirmed the significant hematological effect (P<0.05) of internal parasitism, which was amplified significantly by its interaction with the reproduction stage for HCT and RBC (P<0.01). Monitoring of blood biological constants in ewes during the critical reproductive stages seems to provide valuable data to improve their welfare and resistance to potential internal parasites.
在阿尔及利亚,养羊在很大程度上仍然是传统的,母羊容易患各种疾病,特别是寄生虫病,导致繁殖失败和生产损失。为了评估母羊体内寄生虫(蠕虫和原生动物)及其与不同繁殖阶段和季节的相互作用对血液学参数的影响,在母羊妊娠结束至产后3个月期间(间隔2周)采集了粪便和血液样本(n = 89)。在阿尔及利亚东北部一个地中海气候的地区,动物在传统的以牧场为基础的农业系统下饲养。寄生阴道镜检查结果显示,消化道圆形虫、类圆形虫、鹅毛线虫、莫尼兹虫、肝片吸虫和球虫一致存在。血液学参数,主要是血细胞比容(HCT)、血红蛋白(HGB)和红细胞(RBC),在整个研究期间显示贫血母羊的水平较低。产后第三个月,91%的动物出现了明显的血液学影响(p < 0.05)。混合效应模型的使用证实了内寄生对血液学的显著影响(P<0.05),并通过其与HCT和RBC的繁殖阶段的相互作用显著放大(P<0.01)。监测母羊在关键的繁殖阶段的血液生物学常数似乎提供了有价值的数据,以提高其福利和抵抗潜在的内部寄生虫。
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引用次数: 0
Use of Plectranthus amboinicus in chickens and its effect on productive and economic parameters 安波菌在鸡体内的应用及其对生产经济参数的影响
4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-09-27 DOI: 10.52973/rcfcv-e33295
Angel Roberto Sánchez-Quinche, Adriana Mishel Ibáñez–Loja, Dervin Bartolo Dean–Gutiérrez
This research was carried out at the Santa Inés Farm of the Faculty of Agricultural Sciences of the Universidad Técnica de Machala, El Oro Province, Ecuador. The aim was to evaluate the effect of Plectranthus amboinicus, commonly called oreganon, on the productive and economic parameters of Cobb 500 broilers. For the well–being of the birds, the management established for open house systems in the area was used. A Completely randomized design was applied, where 6 treatments were used, each with 4 Experimental Units of 10 birds, for a total of 240 chickens evaluated. The treatments arrangement was: T1 or control which was based on a commercial type basal diet, while, to the balanced diets of treatments T2, T3, T4 and T5, the dehydrated–ground leaf of P. amboinicus was added at 0.25, 0.50, 0.75 and 1.00%, respectively, in replacement of the Growth Promoter Antibiotic (GPA); the T6 or blank, which did not contain GPA and dehydrated P. amboinicus in the balanced mixture. The variables evaluated were live weight gain (LWG), feed consumption (FC), accumulated water consumption (AWC), feed conversion ratio (FCR), mortality (M), productive efficiency factor (PEF), kg of standing meat per m2, economic expenses and cost per kg of standing meat. For all the variables, an ANOVA was used, previous assumptions of normality and homogeneity, and to discriminate between the means, Tukey's honest significant difference (HSD) procedure was used, with a confidence level of 95%. All data were analyzed using the PROC GLM (General Linear Model) procedure of the SAS statistical package. The results showed that oreganón could works as a replacement alternative to the GPA in broilers, without harming the productive and economic parameters.
这项研究是在厄瓜多尔埃尔奥罗省马查拉大学农业科学学院的Santa insamacs农场进行的。目的是评价羊草对科布500肉鸡生产和经济参数的影响。为了鸟类的健康,在该地区建立了开放式房屋系统的管理。试验采用完全随机设计,共6个处理,每个处理4个实验单元,每组10只鸡,共评估240只鸡。处理安排为:T1或对照,以商品型基础饲粮为基础,在T2、T3、T4和T5处理的平衡饲粮中分别添加0.25、0.50、0.75和1.00%的脱水磨碎叶片,替代生长促进剂抗生素(GPA);T6或空白,不含GPA和脱水的amboinicus平衡混合物。评价变量为活增重(LWG)、饲料消耗量(FC)、累积耗水量(AWC)、饲料系数(FCR)、死亡率(M)、生产效率因子(PEF)、每m2活肉kg、经济费用和每kg活肉成本。对于所有变量,使用方差分析,先前的正态性和均匀性假设,并使用Tukey's诚实显著差异(HSD)程序来区分平均值,置信水平为95%。所有数据均采用SAS统计软件包中的PROC GLM(一般线性模型)程序进行分析。结果表明,oreganón可作为肉仔鸡GPA的替代品,且不影响生产和经济参数。
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引用次数: 0
Morphological characterization of hard palate in the Tabby cats 虎斑猫硬腭的形态学特征
4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-09-27 DOI: 10.52973/rcfcv-e33285
Ayşe Gizem Ermiş, Mehmet Can
The aim of this study was to carry out the morphological characterization of the Tabby cat’s hard palate by light and scanning electron microscopy (SEM), in addition to gross and morphometric analysis. A total of 20 Tabby cat heads used. The materials was provided from the Balıkesir Metropolitan Municipality Street Animals Temporary Nursing Home and Rehabilitation Center, Balıkesir, Turkey. Adult, regardless of gender difference, died from variety of reasons and 20 Tabby cat cadavers which weigh approximately 3–4 kg were brought to the laboratory in accordance with the procedure. Hard palates were subjected to gross examination, morphometric analysis, light and SEM.The Tabby cats hard palate was split up rostral and caudal parts and incisive papilla (papilla incisive), rugae palatine and with a gutter between them, the presence of palatine raphe and secondary rugae palatine were observed. The rostral and caudal parts of the hard palate were determined to have a smooth appearance with SEM 540X magnification and the presence of epithelial desquamations was found out. It was determined that the rostral and caudal part of the Tabby cat’s hard palate had a honeycomb appearance of the microplicae of the 3000X magnification hard palate epithelium with SEM. Round islets were detected with the SEM at the magnification of 600X of the incisive papilla, the 270X of the rostral–caudal parts and the 44X of the gutter between the palatine rugae. These adaptations of the Tabby cat’s hard palate might increase its efficiency during ingestion and mastication of hard food and in directing the food backward. The study shows the detailed anatomical description of the hard palate of the Tabby cat with light and SEM.
本研究的目的是通过光学和扫描电子显微镜(SEM)对虎斑猫的硬腭进行形态学表征,并进行大体和形态计量学分析。总共使用了20个虎斑猫头。这些材料由土耳其Balıkesir的Balıkesir大都会市政街道动物临时疗养院和康复中心提供。成年猫,不分性别,死于各种原因,20具重约3-4公斤的虎斑猫尸体被按照程序带到实验室。硬腭进行大体检查、形态计量学分析、光镜和扫描电镜。虎斑猫的硬腭分为吻侧和尾侧,并有尖锐的乳头(乳头状突起),舌状腭和舌状腭之间有沟槽,存在舌状腭中缝和次级舌状腭。通过扫描电镜(SEM) 540X放大,确定硬腭的吻侧和尾侧部分具有光滑的外观,并发现上皮脱落的存在。结果表明,虎斑猫硬腭吻侧和尾侧的硬腭上皮在3000倍显微镜下呈蜂窝状。扫描电镜在尖锐乳头600X、喙尾部270X、腭褶间沟44X处可见圆形胰岛。虎斑猫对硬腭的这些适应可能会提高它在摄取和咀嚼硬食物时的效率,以及在引导食物倒流时的效率。本研究利用光和扫描电镜对虎斑猫的硬腭进行了详细的解剖描述。
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引用次数: 0
Pronóstico de precios de la carne de cerdo mexicana, con base en el crudo WTI, Maíz y Soya 墨西哥猪肉价格预测,基于WTI原油,玉米和大豆
4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-09-26 DOI: 10.52973/rcfcv-e33250
Sergio Orozco-Cirilo, Juan Manuel Vargas-Canales, Sergio Ernesto Medina–Cuéllar, Juan Antonio Bautista
La investigación realizada es de tipo correlacional y estudió la influencia del precio del barril del crudo WTI, el bushel de Maíz y la tonelada de harina de Soya como variables independientes, sobre el precio de la libra de carne de cerdo mexicana, con el objetivo de hallar una función que explique dichas variaciones. Se utilizaron datos promedios mensuales de los precios, recolectados en un periodo de 10 años desde el 01/10/2012 hasta el 01/09/2022. En los datos hay una clara tendencia lineal entre el precio de la carne de cerdo con el precio del barril de crudo WTI, y el precio de la tonelada de harina de soya, más no tan clara o evidente con el precio del bushel de maíz, siendo esta variable excluida del modelo final por ser estadísticamente no significativa (Sig. 0,184). Las variables independientes del modelo final son estadísticamente significativas (Sig. 0,000), con valores de t–student de 4,999 para el crudo WTI y 3,697 para la harina de soya y no existen problemas de colinealidad entre ellas. El modelo obtenido es de regresión lineal múltiple, y tiene como predictores del precio de la carne de cerdo : el precio del barril de crudo WTI y el precio de la tonelada de harina de soya . Pronostica que el precio de la carne de cerdo no puede descender de 15,50 centavos de USD por libra, y puede explicar las variaciones de esta en un 61,4 %. Los residuos estandarizados del modelo presentan una distribución normal, corroborado mediante una prueba de Kolmogorov–Smirnov de 0,071, existiendo un par valores extremos positivos, que pueden informar sobre las circunstancias de las variables para el interés del investigador en los meses de mayo y junio del año 2021.
correlacional型是调查和研究桶原油价格的影响,WTI蒲式耳的玉米和面粉吨大豆作为独立的变量,对墨西哥的磅猪肉价格,目的是找到一个解释这些变体的作用。使用的是2012年10月1日至2022年9月1日10年期间收集的月度平均价格数据。在数据之间有明确的线性趋势猪肉的价格与桶原油WTI的价格,每吨面粉的价格更为不太明确或明显的大豆,蒲式耳的玉米价格,这是模型的变量不包括最终被0.184统计上不显著(gis)。最终模型的自变量具有统计学意义(Sig. 0.000),粗WTI的t - student值为4999,豆粉的t - student值为3697,它们之间不存在共线性问题。本研究的目的是确定猪肉价格的预测因素,并对猪肉价格的预测因素进行定量分析,以确定猪肉价格的预测因素。他预测,猪肉价格不会从每磅15.50美分下降,并可能解释61.4%的变化。模型标准化废物的正常分布,通过试验证实Kolmogorov—0.071 Smirnov,存在几个积极的极端值,可以报道的关于变量的情况,以引起侦查员在2021年5月和6月。
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引用次数: 0
Plasma profile of Pregnancy Associated Glycoproteins during Postpartum period in Konya Merino ewes lambing single and twin 科尼亚美利奴母羊单羔和双羔产后血浆妊娠相关糖蛋白谱
4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-09-26 DOI: 10.52973/rcfcv-e33298
Eyyup Hakan Ucar, Mehmet Kose, Mesut Kirbas, Faruk Bozkaya, Mehmet Osman Atli
This study investigated the plasma profile of Pregnancy Associated Glycoproteins (PAGs) after lambing in Konya Merino ewes using specific cattle pregnancy test kit. A total of 16 Konya Merino ewes were used as a material. Four groups were set up, ewes birthing a male lamb (SM group, n=4), a female lamb (SF group, n=4), twin male lambs (TM group, n=4) or twin female lambs (TF group, n=4). Blood plasma samples were taken on days 0 (lambing day), 3, 7, 10, 14 and then weekly until day 35. All samples were analysed using a bovine pregnancy test kit to detect of PAGs level. It was determined that there was a strong negative correlation between PAGs levels in peripheral blood and days after lambing (r2=0.969; P<0.01). However, no relationship was found between PAGs level and lamb gender or birth type. In conclusion, plasma PAGs level decreases rapidly in Konya Merino ewes regardless of lamb gender and birth type. Moreover, the results showed that the plasma profile of PAGs after lambing in Konya Merino ewes can be monitored with a bovine commercial ELISA–based pregnancy test kit, and the test results can be used in decisions and assessments based on the levels of PAG molecules.
本研究采用专用的牛妊娠试验试剂盒对科尼亚美利奴母羊产羔后血浆妊娠相关糖蛋白(妊娠相关糖蛋白)进行了检测。以16头科尼亚美利奴母羊为材料。将母羊分为4组,分别产下1只公羊羔(SM组,n=4)、1只母羊羔(SF组,n=4)、2只公羊羔(TM组,n=4)和2只母羊羔(TF组,n=4)。在第0天(产羔日)、第3天、第7天、第10天、第14天采集血浆,然后每周采集一次,直至第35天。所有样品均采用牛妊娠试验试剂盒检测PAGs水平。结果表明,仔猪外周血PAGs水平与产羔后天数呈极显著负相关(r2=0.969;术中,0.01)。但PAGs水平与羔羊性别、出生类型无显著关系。综上所述,无论羔羊性别和出生类型,科尼亚美利奴母羊血浆PAGs水平均迅速下降。此外,结果表明,基于牛elisa的商业妊娠试验试剂盒可以监测科尼亚美利奴母羊产羔后血浆中PAG的分布,检测结果可用于基于PAG分子水平的决策和评估。
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引用次数: 0
Investigation of changes in Hematological parameters and levels of Oxidative stress factors in castrated Cats 去势猫血液学参数及氧化应激因子水平变化的研究
4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-09-25 DOI: 10.52973/rcfcv-e33290
Eren Polat, Tutku Can Acisu, Mustafa Sönmez, Emine Ünsaldi, Önder Otlu, Murat Tanrisever, Aslıhan Çakır, Yasin Baykalir
This study was carried out to determine the effect of castration procedure on hemogram and leukogram values and the levels of oxidative stress factors in serum tissue in cats. Preoperative and postoperative 10th day hemogram/leukogram values and total oxidant/antioxidant capacities of 19 castrated cats were evaluated in the study. When the hemogram and leukogram values were examined, it was determined that there was a statistically significant increase in the amount of eosinophils (P<0.05). Although the increase in white blood cells (WBC), neutrophil and lymphocyte levels and the decrease in red blood cells (RBC) and hematocrit (HCT) levels were remarkable, it was not found to be statistically significant (P>0.05). When the levels of oxidative stress factors in the serum tissues of the cats were examined, it was determined that there was a decrease in the total antioxidant status (TAS) and an increase in the total oxidant status (TOS), but the difference in the results was not statistically significant (P>0.05). In conclusion, in this study, it was determined that the castration procedure in cats did not cause a significant difference in hemogram and leukogram parameters and the level of oxidative stress factors in serum tissue, except for the amount of eosinophils.
本研究旨在确定去势手术对猫血象和白象值以及血清组织氧化应激因子水平的影响。研究评估了19只去势猫术前和术后第10天血象/白象值和总氧化剂/抗氧化能力。当血象和白象值检查时,确定嗜酸性粒细胞数量有统计学意义的增加(P<0.05)。虽然白细胞(WBC)、中性粒细胞和淋巴细胞水平升高,红细胞(RBC)和红细胞压积(HCT)水平下降,但差异无统计学意义(P>0.05)。检测猫血清组织中氧化应激因子水平时,发现总抗氧化状态(TAS)降低,总氧化状态(TOS)升高,但结果差异无统计学意义(P>0.05)。综上所述,本研究确定,除嗜酸性粒细胞数量外,阉割过程对猫血象和白象参数以及血清组织中氧化应激因子水平没有显著影响。
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引用次数: 0
Detection of Brucella canis infection in Pit Bull breed dogs in Turkey 土耳其比特斗牛犬布鲁氏菌感染检测
4区 农林科学 Q4 VETERINARY SCIENCES Pub Date : 2023-09-24 DOI: 10.52973/rcfcv-e33304
Volkan Özavci, Hafize Tuğba Yüksel Dolgun, Yiğit Seferoğlu, Şükrü Kirkan
Brucella canis infection is an often neglected but important zoonotic disease. This study aims to determine its seroprevalence in Pit Bull dogs from the Western Region of the Turkish Anatolian Peninsula. In the Province of Manisa, 2 mL blood samples were taken from the antebrachial region of 35 Pit Bull dogs using sterile K2EDTA (3.6 mg) blood tubes, and the samples were analyzed using both the mercaptoethanol (ME) microagglutination test and B. canis–specific PCR techniques. Of the 35 dogs tested by 2–ME RSAT, 13 (37.14%) tested positive and 22 (63%) tested negative. Of the 13 dogs that tested positive for 2–ME RSAT, 8 (22.85%) were female, and 5 (14.28%) were male. Subsequent PCR analysis of all samples revealed that 7 (20%; 7/35) of the samples that tested positive for 2–ME RSAT were actually B. canis–specific PCR positive. These findings suggest that B. canis is present in Pit Bull dogs, although they provide a general idea of the disease's prevalence of the disease in the region. Multicentre studies with larger numbers of cases in different groups of Pit Bulls, such as healthy, patient and risk groups, are needed to provide comprehensive evidence.
犬布鲁氏菌感染是一种常被忽视但重要的人畜共患疾病。本研究旨在确定其在土耳其安纳托利亚半岛西部地区比特斗牛犬的血清患病率。在马尼萨省,使用无菌K2EDTA (3.6 mg)血管从35只比特斗牛犬的前肢区域采集了2 mL血液样本,并使用巯基乙醇(ME)微凝集试验和犬链球菌特异性PCR技术对样本进行了分析。35只狗接受2-ME RSAT检测,阳性13只(37.14%),阴性22只(63%)。在13只2-ME RSAT检测阳性的狗中,雌性8只(22.85%),雄性5只(14.28%)。随后对所有样本进行PCR分析,发现7 (20%;7/35) 2-ME RSAT检测阳性的样本实际上是犬b特异性PCR阳性。这些发现表明犬B. canis存在于比特斗牛犬中,尽管它们提供了该疾病在该地区流行的总体概念。为了提供全面的证据,需要对不同群体的比特犬进行多中心研究,包括健康、病人和危险群体。
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引用次数: 0
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Revista Cientifica-Facultad De Ciencias Veterinarias
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