蓝花叶提取物通过抗氧化机制和上调StAR基因表达维持高胆固醇血症大鼠睾酮水平

Q3 Pharmacology, Toxicology and Pharmaceutics Biomedical and Pharmacology Journal Pub Date : 2023-09-30 DOI:10.13005/bpj/2724
I Gede Widhiantara, Putu Angga Wiradana, Anak Agung Ayu Putri Permatasari, Ni Kadek Yunita Sari, I Wayan Rosiana, Ni Putu Widya Astuti, Luh Putu Widiastini, I Made Jawi, I Wayan Putu Sutirta Yasa
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引用次数: 0

摘要

高胆固醇水平会增加组织中的脂质过氧化,这对生殖器官细胞,特别是产生睾酮激素的间质细胞有潜在的毒性。苦瓜叶提取物(blmea balsamifera leaf extract, BBLE)的主要成分是具有抗高胆固醇活性的类黄酮化合物。本研究旨在确定给药BBLE对高胆固醇血症大鼠MDA水平、StAR mRNA表达、间质细胞计数和睾酮水平的影响。本研究采用纯后测对照组设计。将36只雄性Wistar大鼠分为两组:1)对照组(HCD + 1 ml/d无菌aquadest)和2)BBLE组(HCD + 4 mg/b /d)。治疗后,检测两组大鼠睾丸组织MDA水平、StAR mRNA表达、间质细胞计数及睾酮水平。收集的数据采用独立t检验和通径分析进行统计学检验。结果显示,BBLE组MDA水平较低,但StAR基因表达、间质细胞计数和睾酮水平显著高于对照组(p0.05)。StAR mRNA的表达对睾酮水平有显著的直接影响。研究表明,在高胆固醇血症大鼠中,给药BBLE可以通过防止睾丸组织氧化应激(MDA水平降低,StAR mRNA上调,间质细胞再生)来改善睾丸激素分泌。
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Blumea balsamifera Leaf Extract Maintain Testosterone Levels in Hypercholesterolemic Rats Through Antioxidant Mechanism and Upregulation of StAR Gene Expression
High cholesterol levels can increase lipid peroxidation in tissues that are potentially toxic to reproductive organ cells, especially the Leydig cells that produce the testosterone hormone. Blumea balsamifera leaf extract (BBLE) has the main content in the form of flavonoid compounds with antihypercholesterolemic activity. This study aimed to determine the effect of BBLE administration on MDA levels, StAR mRNA expression, Leydig cell counts, and testosterone levels in hypercholesterolemic rats. A posttest-only control group design was utilized in this research. For 50 days, 36 male Wistar rats had been separated into two groups: 1) the control group (HCD + 1 ml/day sterile aquadest) and 2) the BBLE group (HCD + 4 mg/bb rats per day). After treatment, MDA testicular tissue levels, StAR mRNA expression, Leydig cell count, and testosterone levels were measured in both groups. The data collected were statistically examined using the Independent T-test and path analysis. The results indicated that the MDA level was lower in the BBLE group, though StAR gene expression, Leydig cell count, and testosterone levels were significantly greater (p0.05). StAR mRNA expression had a significant direct effect on testosterone levels. Administration of BBLE had been shown to improve testosterone hormone secretion in hypercholesterolemic rats by preventing oxidative stress in testicular tissue with the signs of lower MDA levels, up-regulation of the StAR mRNA, and Leydig cell regeneration.
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来源期刊
Biomedical and Pharmacology Journal
Biomedical and Pharmacology Journal Pharmacology, Toxicology and Pharmaceutics-Pharmacology
CiteScore
1.20
自引率
0.00%
发文量
189
期刊介绍: Biomedical and Pharmacology Journal (BPJ) is an International Peer Reviewed Research Journal in English language whose frequency is quarterly. The journal seeks to promote research, exchange of scientific information, consideration of regulatory mechanisms that affect drug development and utilization, and medical education. BPJ take much care in making your article published without much delay with your kind cooperation and support. Research papers, review articles, short communications, news are welcomed provided they demonstrate new findings of relevance to the field as a whole. All articles will be peer-reviewed and will find a place in Biomedical and Pharmacology Journal based on the merit and innovativeness of the research work. BPJ hopes that Researchers, Research scholars, Academician, Industrialists etc. would make use of this journal for the development of science and technology. Topics of interest include, but are not limited to: Biochemistry Genetics Microbiology and virology Molecular, cellular and cancer biology Neurosciences Pharmacology Drug Discovery Cardiovascular Pharmacology Neuropharmacology Molecular & Cellular Mechanisms Immunology & Inflammation Pharmacy.
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