骆驼toll样受体2基因的分子特征

IF 0.2 4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Indian Journal of Animal Sciences Pub Date : 2023-08-02 DOI:10.56093/ijans.v92i7.125173
RAM KUMAR GAHLOT, BASANTI JYOTSANA, VED PRAKASH, SHIRISH DADARAO NARNAWARE, N S RATHORE
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引用次数: 0

摘要

toll样受体(TLRs)是一个重要的受体家族,构成了第一道防线。toll样受体通过识别来自各种微生物的病原体相关分子模式,在先天免疫系统中起着至关重要的作用。本文对印度单峰骆驼TLR2基因的编码序列进行了研究。采集了5只Bikaneri骆驼的血液,并分离了基因组DNA。设计了8对重叠引物,用于扩增TLR2基因编码区。所有片段均经聚合酶链反应(PCR)扩增成功。PCR产物纯化后进行Sanger测序。成功扩增出全长3213 bp的TLR2基因片段,其中包括2287 bp的外显子编码序列和926 bp的3 '非翻译区(UTR)。生成的序列提交给NCBI,登记号为MW557315.1。所编码的氨基酸编码12个富含亮氨酸的重复序列(LRR)。UBN09134.1)。在核苷酸水平上,骆驼TLR2基因与骆驼类相似性最高(99.30 ~ 99.59%),其次是骆马(97.54%)。与智人核苷酸序列相似性最低(82.37%)。基于氨基酸序列的系统进化树显示,骆驼与猪的亲缘关系较近(86.2%),人类、大猩猩、马和驴的亲缘关系较远。
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Molecular characterization of toll-like receptor 2 gene in Bikaneri camels (Camelus dromedarius)
Toll-like receptors (TLRs) are an important family of receptors constituting the first line of defense system. Tolllike receptors play crucial roles in the innate immune system by recognizing pathogen-associated molecular patterns derived from various microbes. Present investigation was carried out to characterize the coding sequence of TLR2 gene in Indian dromedary camels. Blood was collected from five Bikaneri camels and genomic DNA was isolated. Eight pairs of overlapping primers were designed for amplification of TLR2 gene coding region. All the fragments were successfully amplified using Polymerase chain reaction (PCR). The PCR products were purified and subjected to Sanger’s sequencing. TLR2 gene fragment of 3213 bp consisting of 2287 bp of coding sequence of exon 2 and 926 bp of 3’untranslated region (UTR) was successfully amplified. The sequence generated was submitted to the NCBI with accession number MW557315.1. The encoded amino acids codes for 12 leucine rich repeats (LRR) (Accession no. UBN09134.1). At the nucleotide level, the Bikaneri camels’ TLR2 gene showed highest similarity with camelids (99.30 to 99.59%) followed by vicugna pacos (97.54%). The nucleotide sequence similarity was lowest with Homo sapiens (82.37%). The amino acid sequence based phylogenetic tree revealed close relationship between camelid species followed by pig (86.2%), whereas human, gorilla, horse and donkey were distantly placed on the tree.
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来源期刊
Indian Journal of Animal Sciences
Indian Journal of Animal Sciences 农林科学-奶制品与动物科学
CiteScore
0.60
自引率
25.00%
发文量
220
审稿时长
8 months
期刊介绍: Articles published in The Indian Journal of Animal Sciences encompass a broad range of research topics in animal health and production related to cattle, buffalo, sheep, goat, camel, equines, pig, rabbit, yak, mithun, poultry and fisheries. Studies involving wildlife species and laboratory animal species that address fundamental questions about their biology will also be considered for publication. All manuscripts must present some new development and must be original, timely, significant and scientifically excellent. Papers will be rejected if standards of care of, or procedures performed on animals are not up to those expected of humane veterinary scientists. At a minimum, standards must meet the International Guiding Principles for Biomedical Research involving Animals, as issued by the Council for International Organizations of Medical Sciences. (C.I.O.M.S., c/o WHO, CH 1211 Geneva 27, Switzerland). Articles reporting new animal disease must follow GOI directive as given in detail in Guidelines to Authors.
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