基于共生性能的缓生根瘤菌菌株遗传多样性评价与选择

IF 0.3 4区 农林科学 Q4 AGRICULTURE, MULTIDISCIPLINARY Indian Journal of Agricultural Sciences Pub Date : 2023-10-09 DOI:10.56093/ijas.v93i10.140808
SANTOSH KUMAR, PREETI SINGH, SHIV CHARAN KUMAR, NITISH RANJAN PRAKASH, UMAKANT BANJARE, ARUN KUMAR PATEL, VISHAL TYAGI, RAMESH KUMAR SINGH
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引用次数: 0

摘要

鸽豆(Cajanus cajan (L.))的共生关系与根瘤菌共生的Millsp.)在植物的生长发育中起着至关重要的作用。本研究于2018-2020年在北方邦瓦拉纳西巴纳拉斯印度大学农业科学研究所进行,旨在探索在Inceptisols下Cajanus cajan根中存在的根瘤菌多样性,并鉴定出一种具有特殊植物生长促进作用和高效固氮酶活性的精英菌株。16s rDNA测序鉴定菌株为:日本慢根瘤菌(S12)、地下慢根瘤菌(S1、S7、S8、S10、S13)、远明慢根瘤菌(S2、S3、S4、S6、S9、S11),内生菌鉴定为:偶氮假单胞菌(S5)和塞多纳芽孢杆菌(S15)。UPGMA聚类结果显示,14株菌株的遗传多样性存在显著差异,可分为3个一级聚类和4个二级聚类。菌株S1、S3、S6、S9和S13表现出较高的固氮酶活性,表明它们具有较强的固氮能力。在植物生长试验中,所有菌株均表现出较对照显著的植株生长。这5个菌株的显著生长促进可归因于其显著的固氮效率,如显著提高根瘤形成和氮酶活性。这些发现为确定潜在的根瘤菌接种剂以提高鸽豆产量提供了有价值的见解。
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Genetic diversity assessment and selection of Bradyrhizobium strains for Inceptisols based on symbiotic performance
The symbiotic association of pigeonpea (Cajanus cajan (L.) Millsp.) with rhizobia plays a pivotal role in plants’ growth and development. The present study was carried out at Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh during 2018–2020 to explore the diversity of rhizobia present in the Cajanus cajan root under Inceptisols and identify an elite strain that exhibits exceptional plant growth promotion and of nitrogenase activity for efficient nitrogen fixation. The bacterial identification using 16s rDNA sequencing revealed bacteria strains Bradyrhizobium japonicum (S12), Bradyrhizobium subterraneum (S1, S7, S8, S10, S13), Bradyrhizobium yuanmingense (S2, S3, S4, S6, S9, S11) while two bacteria were the endophytes identified as Pseudomonas azotoformans (S5) and Paenibacillus seodonensis (S15). Genetic diversity using UPGMA clustering revealed remarkable genetic variations, classifying 14 strains into three primary clusters and four secondary clusters. Strains S1, S3, S6, S9, and S13 exhibited elevated nitrogenase activity, indicative of their proficient nitrogen fixation capability. In plant growth experiments, all strains demonstrated significant growth of the plants compared to control. The notable growth enhancement by these five strains can be attributed to their remarkable efficiency in nitrogen fixation, as indicated by significantly higher nodule formation and nitrogenase activity. These findings provide valuable insights for identifying potential rhizobial inoculants to enhance pigeonpea productivity.
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来源期刊
Indian Journal of Agricultural Sciences
Indian Journal of Agricultural Sciences 农林科学-农业综合
CiteScore
0.80
自引率
25.00%
发文量
273
审稿时长
6 months
期刊介绍: The Indian Journal of Agricultural Sciences publishes papers concerned with the advancement of agriculture throughout the world. It publishes original scientific work related to strategic and applied studies in all aspects of agricultural science and exploited species, as well as reviews of scientific topics of current agricultural relevance. Specific topics of interest include (but are not confined to): genetic resources, all aspects of crop improvement,crop production,crop protection, physiology, modeling of crop systems, the scientific underpinning of agronomy, engineering solutions, decision support systems, land use, environmental impacts of agriculture and forestry, impacts of climate change, rural biodiversity, experimental design and statistical analysis, the application of new analytical and study methods (including molecular studies) and agricultural economics. The journal also publishes book reviews. Articles are accepted on the following broad disciplines: Agric. Engineering & Technology, Agric. Social & Economic Sci., Agronomy, Biometry, Biosciences, Cytology, Ecology, Environmental Sciences, Fertilization, Forestry , Genetics, Horticultural Sciences, Microbiology, Pest, Weed Control etc., Molecular Biology, Plant Pathology, Plant Breeding, Physiology and Biochemistry, Soil Sciences, Special Cultivation Technology, Stress Breeding, Agric. extension, and Cell Biology.
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