{"title":"晚熟桃(<i>Prunus persica</i>)遗传多样性及亲缘关系分析L.用ISSR和SRAP标记评价品种","authors":"Wen Li, Yujie Ma, Yidan Kou, Zhifang Zeng, Dongliang Qiu, Cuilan Ma","doi":"10.48130/frures-2023-0036","DOIUrl":null,"url":null,"abstract":"Molecular characterization of 39 late-mature peach (<italic>Prunus persica</italic> L.) accessions was carried out using the ISSR (inter simple sequence repeats) and SRAP (sequence-related amplified polymorphism) markers to assess the value and magnitude of genetic divergence. The ISSR primers revealed 70.09% polymorphism and the SRAP marker generated 73.33% polymorphism. Pooled ISSR and SRAP, along with UPGMA clustering based on similarity coefficients, were estimated to assess the efficiency of the marker system in peaches. Similarity coefficients between accessions regarding the ISSR and SRAP markers ranged from 0.65 to 0.89, indicating a broad genetic peach base. A maximum similarity coefficient of 0.89 was observed between C12 'Weiduanmihong' (Fujian Yikangyuan Farm Co., Ltd., 26.58°N, 118.75°E) and C32 'Weiduanmihong' (WeiduanVillage, 26.58°N, 118.75°E), and a minimum of 0.65 was observed in C6 'Huangjinmitao 5' (Gutian Natural Pantaoyuan Family Farm, 26.58°N, 118.75°E) and the rest of the genotypes. The present study found that a high level of polymorphism indicated their applicability in framing more extensive studies to develop superior progeny, conduct molecular breeding, investigate genetic population diversity, make comparative maps, select parents, etc., in various peach crop improvement programs.","PeriodicalId":37667,"journal":{"name":"Fruit Growing Research","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Analysis of genetic diversity and relationships between late-mature peach (<i>Prunus persica</i> L.) varieties assessed with ISSR and SRAP Markers\",\"authors\":\"Wen Li, Yujie Ma, Yidan Kou, Zhifang Zeng, Dongliang Qiu, Cuilan Ma\",\"doi\":\"10.48130/frures-2023-0036\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Molecular characterization of 39 late-mature peach (<italic>Prunus persica</italic> L.) accessions was carried out using the ISSR (inter simple sequence repeats) and SRAP (sequence-related amplified polymorphism) markers to assess the value and magnitude of genetic divergence. The ISSR primers revealed 70.09% polymorphism and the SRAP marker generated 73.33% polymorphism. Pooled ISSR and SRAP, along with UPGMA clustering based on similarity coefficients, were estimated to assess the efficiency of the marker system in peaches. Similarity coefficients between accessions regarding the ISSR and SRAP markers ranged from 0.65 to 0.89, indicating a broad genetic peach base. A maximum similarity coefficient of 0.89 was observed between C12 'Weiduanmihong' (Fujian Yikangyuan Farm Co., Ltd., 26.58°N, 118.75°E) and C32 'Weiduanmihong' (WeiduanVillage, 26.58°N, 118.75°E), and a minimum of 0.65 was observed in C6 'Huangjinmitao 5' (Gutian Natural Pantaoyuan Family Farm, 26.58°N, 118.75°E) and the rest of the genotypes. The present study found that a high level of polymorphism indicated their applicability in framing more extensive studies to develop superior progeny, conduct molecular breeding, investigate genetic population diversity, make comparative maps, select parents, etc., in various peach crop improvement programs.\",\"PeriodicalId\":37667,\"journal\":{\"name\":\"Fruit Growing Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Fruit Growing Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.48130/frures-2023-0036\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fruit Growing Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.48130/frures-2023-0036","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
Analysis of genetic diversity and relationships between late-mature peach (<i>Prunus persica</i> L.) varieties assessed with ISSR and SRAP Markers
Molecular characterization of 39 late-mature peach (Prunus persica L.) accessions was carried out using the ISSR (inter simple sequence repeats) and SRAP (sequence-related amplified polymorphism) markers to assess the value and magnitude of genetic divergence. The ISSR primers revealed 70.09% polymorphism and the SRAP marker generated 73.33% polymorphism. Pooled ISSR and SRAP, along with UPGMA clustering based on similarity coefficients, were estimated to assess the efficiency of the marker system in peaches. Similarity coefficients between accessions regarding the ISSR and SRAP markers ranged from 0.65 to 0.89, indicating a broad genetic peach base. A maximum similarity coefficient of 0.89 was observed between C12 'Weiduanmihong' (Fujian Yikangyuan Farm Co., Ltd., 26.58°N, 118.75°E) and C32 'Weiduanmihong' (WeiduanVillage, 26.58°N, 118.75°E), and a minimum of 0.65 was observed in C6 'Huangjinmitao 5' (Gutian Natural Pantaoyuan Family Farm, 26.58°N, 118.75°E) and the rest of the genotypes. The present study found that a high level of polymorphism indicated their applicability in framing more extensive studies to develop superior progeny, conduct molecular breeding, investigate genetic population diversity, make comparative maps, select parents, etc., in various peach crop improvement programs.
期刊介绍：
FRUIT GROWING RESEARCH is an international journal, which has been issued under the care of the Research Institute for Fruit Growing Pitesti, Romania. Fruit growing research is an annual journal aimed at disseminating significant research and original papers on genetic resources, breeding of deciduous fruit trees species; fruit sustainable technologies – environment protection; propagation, virology and tissue culture, postharvest, that can be of interest to a wide audience of plant scientists in all areas of fruit growing. An international Editorial Board advises the journal.
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