podoplanin条件敲除小鼠骨细胞形态学研究

IF 0.3 4区 医学 Q4 ENGINEERING, BIOMEDICAL Journal of Hard Tissue Biology Pub Date : 2023-01-01 DOI:10.2485/jhtb.32.213
Kyoko Osawa, Takenori Kanai, Natsumi Ushijima, Koichiro Kajiwara, Yoshihiko Sawa, Yoshiaki Sato
{"title":"podoplanin条件敲除小鼠骨细胞形态学研究","authors":"Kyoko Osawa, Takenori Kanai, Natsumi Ushijima, Koichiro Kajiwara, Yoshihiko Sawa, Yoshiaki Sato","doi":"10.2485/jhtb.32.213","DOIUrl":null,"url":null,"abstract":"We generated podoplanin-conditional knockout mice where the floxed podoplanin exon3 was deleted by the Dmp1-driven Cre (Dmp1-Cre;PdpnΔ/Δ) and investigated the cell process elongation of podoplanin-deficient mouse osteocyte in vitro and in vivo. The expression of podoplanin is found in odontoblasts while not observed in odontoblasts of Dmp1-Cre;PdpnΔ/Δ mice, indicating that the conditional knockout of podoplanin in Dmp1-expressing cells in Dmp1-Cre;PdpnΔ/Δ mice is successful. There were no differences in the growth of wild-type and Dmp1-Cre;PdpnΔ/Δ mice, and no differences in calcification and alkaline phosphatase activity in cultured calvarial osteoblasts of the wild-type and Dmp1-Cre;PdpnΔ/Δ mice, in total this suggests that the podoplanin-cKO has no effect on generation of the bone. The cell process elongation was suppressed in cultured calvarial osteoblasts of Dmp1-Cre;PdpnΔ/Δ mice compared with wild-type mice. In the electron microscopic study, there were no morphological differences in bone matrix formation and osteocyte distribution in Dmp1-Cre;PdpnΔ/Δ and wild-type mice, whereas the cell process formation was sparser and the network with neighboring cells was more deficient in Dmp1-Cre;PdpnΔ/Δ mice than in wild-type mice. In the quantitative analysis, the number and thickness of the cell processes were significantly smaller and thinner in Dmp1-Cre;PdpnΔ/Δ mice than in wild-type mice. This could suggest that podoplanin plays a role in the formation of the osteocyte network created by the cell process elongation.","PeriodicalId":16040,"journal":{"name":"Journal of Hard Tissue Biology","volume":null,"pages":null},"PeriodicalIF":0.3000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Morphological Study for the Osteocytes in Podoplanin-Conditional Knockout Mice\",\"authors\":\"Kyoko Osawa, Takenori Kanai, Natsumi Ushijima, Koichiro Kajiwara, Yoshihiko Sawa, Yoshiaki Sato\",\"doi\":\"10.2485/jhtb.32.213\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"We generated podoplanin-conditional knockout mice where the floxed podoplanin exon3 was deleted by the Dmp1-driven Cre (Dmp1-Cre;PdpnΔ/Δ) and investigated the cell process elongation of podoplanin-deficient mouse osteocyte in vitro and in vivo. The expression of podoplanin is found in odontoblasts while not observed in odontoblasts of Dmp1-Cre;PdpnΔ/Δ mice, indicating that the conditional knockout of podoplanin in Dmp1-expressing cells in Dmp1-Cre;PdpnΔ/Δ mice is successful. There were no differences in the growth of wild-type and Dmp1-Cre;PdpnΔ/Δ mice, and no differences in calcification and alkaline phosphatase activity in cultured calvarial osteoblasts of the wild-type and Dmp1-Cre;PdpnΔ/Δ mice, in total this suggests that the podoplanin-cKO has no effect on generation of the bone. The cell process elongation was suppressed in cultured calvarial osteoblasts of Dmp1-Cre;PdpnΔ/Δ mice compared with wild-type mice. In the electron microscopic study, there were no morphological differences in bone matrix formation and osteocyte distribution in Dmp1-Cre;PdpnΔ/Δ and wild-type mice, whereas the cell process formation was sparser and the network with neighboring cells was more deficient in Dmp1-Cre;PdpnΔ/Δ mice than in wild-type mice. In the quantitative analysis, the number and thickness of the cell processes were significantly smaller and thinner in Dmp1-Cre;PdpnΔ/Δ mice than in wild-type mice. This could suggest that podoplanin plays a role in the formation of the osteocyte network created by the cell process elongation.\",\"PeriodicalId\":16040,\"journal\":{\"name\":\"Journal of Hard Tissue Biology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.3000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Hard Tissue Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2485/jhtb.32.213\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"ENGINEERING, BIOMEDICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Hard Tissue Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2485/jhtb.32.213","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
引用次数: 0

摘要

我们通过dmp1驱动的Cre (Dmp1-Cre;PdpnΔ/Δ)删除了带有floxed的podoplanin外显子3,生成了podoplanin条件敲除小鼠,并在体外和体内研究了podoplanin缺陷小鼠骨细胞的细胞过程延长。在Dmp1-Cre;PdpnΔ/Δ小鼠成牙细胞中未发现podoplanin的表达,说明在Dmp1-Cre;PdpnΔ/Δ小鼠dmp1表达细胞中条件敲除podoplanin是成功的。野生型和Dmp1-Cre;PdpnΔ/Δ小鼠的生长无差异,野生型和Dmp1-Cre;PdpnΔ/Δ小鼠培养的颅骨成骨细胞的钙化和碱性磷酸酶活性无差异,这表明podoplanin-cKO对骨的生成没有影响。与野生型小鼠相比,Dmp1-Cre;PdpnΔ/Δ小鼠培养的颅骨成骨细胞的细胞过程伸长受到抑制。电镜观察发现,Dmp1-Cre;PdpnΔ/Δ与野生型小鼠在骨基质形成和骨细胞分布方面没有形态学上的差异,而Dmp1-Cre;PdpnΔ/Δ小鼠的细胞过程形成较野生型小鼠更稀疏,与邻近细胞的网络更缺乏。在定量分析中,Dmp1-Cre;PdpnΔ/Δ小鼠细胞突的数量和厚度明显小于野生型小鼠。这可能表明podoplanin在由细胞过程伸长产生的骨细胞网络的形成中起作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Morphological Study for the Osteocytes in Podoplanin-Conditional Knockout Mice
We generated podoplanin-conditional knockout mice where the floxed podoplanin exon3 was deleted by the Dmp1-driven Cre (Dmp1-Cre;PdpnΔ/Δ) and investigated the cell process elongation of podoplanin-deficient mouse osteocyte in vitro and in vivo. The expression of podoplanin is found in odontoblasts while not observed in odontoblasts of Dmp1-Cre;PdpnΔ/Δ mice, indicating that the conditional knockout of podoplanin in Dmp1-expressing cells in Dmp1-Cre;PdpnΔ/Δ mice is successful. There were no differences in the growth of wild-type and Dmp1-Cre;PdpnΔ/Δ mice, and no differences in calcification and alkaline phosphatase activity in cultured calvarial osteoblasts of the wild-type and Dmp1-Cre;PdpnΔ/Δ mice, in total this suggests that the podoplanin-cKO has no effect on generation of the bone. The cell process elongation was suppressed in cultured calvarial osteoblasts of Dmp1-Cre;PdpnΔ/Δ mice compared with wild-type mice. In the electron microscopic study, there were no morphological differences in bone matrix formation and osteocyte distribution in Dmp1-Cre;PdpnΔ/Δ and wild-type mice, whereas the cell process formation was sparser and the network with neighboring cells was more deficient in Dmp1-Cre;PdpnΔ/Δ mice than in wild-type mice. In the quantitative analysis, the number and thickness of the cell processes were significantly smaller and thinner in Dmp1-Cre;PdpnΔ/Δ mice than in wild-type mice. This could suggest that podoplanin plays a role in the formation of the osteocyte network created by the cell process elongation.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of Hard Tissue Biology
Journal of Hard Tissue Biology ENGINEERING, BIOMEDICAL-
CiteScore
0.90
自引率
0.00%
发文量
28
审稿时长
6-12 weeks
期刊介绍: Information not localized
期刊最新文献
Evaluation of Maxillary First Molar Intrusion Mechanics with Mini-Implant Anchorages Using the Finite Element Method Study on the Effect of Soft-Start Light on Microleakage in Pit and Fissure Closure Upregulation of miR-101-3p Overcomes Ibrutinib Resistance by Targeting ABCC5 in Diffuse Large B-Cell Lymphoma (DLBCL) miR-141 Improve Osteoporosis by Promoting Osteoblast Differentiation through Targeting RICTOR A Study of Submandibular Gland Changes in Mice of a Murine Model of Sjögren’s Syndrome Administered Dental Pulp Stem Cell-Conditioned Medium
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1