灌肠虫的鉴定、生物学特性及半人工栽培试验

Qinlan Guan, Ming-Fu Gong, Zhongqin Ma, Chenxin Lu
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引用次数: 0

摘要

灌肠虫(L.)p . Kumm。是一种外生菌根真菌,与李(Prunus mume)共生。本文采用形态学和分子学方法对一株赤羊绦虫进行了鉴定。对菌株的生物学特性进行了观察,并进行了半人工培养试验。结果表明,菌株MBQM10168鉴定为灌肠虫。不同培养基、pH、生长温度、碳氮源、透气性等条件下,灌肠草的形态、生长、菌丝密度等指标存在显著差异。然而,它的生长对光不敏感。在特定的碳源和氮源的条件下,以棉籽壳为培养基,在pH值为4 ~ 8、温度为18 ~ 28℃的条件下,以梅叶分枝为培养基,培养红叶菊菌丝。其中包括纤维素二糖、蔗糖和醋酸钠作为碳源,赖氨酸、柠檬酸二铵、蛋氨酸和硫酸铵作为氮源。另外,用棉塞进行密封的方法。综上所述,本研究证明了在适宜条件下半人工栽培灌丛草的可行性,有望促进灌丛草种植面积的综合利用,提高灌丛草的经济价值。
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Identification, Biological Characteristics and Semi Artificial Cultivation Experiment of Entoloma clypeatum
Entoloma clypeatum (L.) P. Kumm., also known as Rhodophyllus clypeatus (L. fr.) Quel, is an ectomycorrhizal fungus that grows with Prunus mume . In this study, a strain of E. clypeatum was identified and characterized using morphological and molecular methods. Furthermore, the biological characteristics of the strain were observed, and a semi artificial cultivation experiment was conducted. The results showed that, the strain MBQM10168 was identified to be Entoloma clypeatum . The indices including the morphology, growth, and mycelial density of E. clypeatum exhibited significant differences under different conditions such as culture media, pH, growth temperature, carbon and nitrogen sources, and air permeability. However, it’s growth was not sensitive to light. The optimal growth conditions for E. clypeatum mycelium were cultured with branches of P. mume and cottonseed hulls culture media, with a pH range of 4–8, temperature range of 18–28 °C, along-side specific sources of carbon and nitrogen. These include cellobiose, sucrose, and sodium acetate as carbon sources, and lysine, diammonium citrate, methionine, and ammonium sulfate as nitrogen sources. In addition, with cotton plug for sealing method. Overall, this study demonstrated the feasibility of semi-artificial cultivation of E. clypeatum under suitable conditions, which is expected to promote the comprehensive utilization of the P. mume plant area and improve the economic values.
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来源期刊
Journal of Biobased Materials and Bioenergy
Journal of Biobased Materials and Bioenergy 工程技术-材料科学:生物材料
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60
审稿时长
6 months
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