肌痛性脑脊髓炎/慢性疲劳综合征(ME/CFS)外周血单个核细胞的生物阻抗谱表征

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摘要

肌痛性脑脊髓炎/慢性疲劳综合征(ME/CFS)是一种致残性慢性疾病,与当前的COVID-19大流行有重要关系。目前,没有专门的实验室测试来直接诊断ME/CFS。本文探讨了阻抗谱技术在ME/CFS诊断中的应用前景。设计并实现了一种用于外周血单个核细胞电学表征的专用装置。用1M浓度的氯化钠溶液对样品施加渗透应力后,在1 Hz ~ 500 MHz范围内进行阻抗谱测量。分析了在1.36 kHz和154 kHz两个特定频率下渗透胁迫后的时间演变。该装置对细胞的存在和渗透过程的演变具有敏感性。与对照样本相比,在1.36 kHz时,ME/CFS患者的阻抗值更高(实部和虚部均约为15%)。在154千赫频率下,患者样本和对照样本之间没有发现显著差异。结果有助于进一步了解ME/CFS患者的诊断及其血液样本与生物阻抗测量的关系。
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Bioimpedance spectroscopy characterization of Myalgic Encephalomyelitis/ Chronic Fatigue Syndrome (ME/CFS) peripheral blood mononuclear cells
Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is a disabling and chronic disease, importantly related to the current COVID-19 pandemic. Currently, there are no specific laboratory tests to directly diagnose ME/CFS. In this work, the use of impedance spectroscopy is studied as a potential technique for the diagnosis of ME/CFS. A specific device for the electrical characterization of peripheral blood mononuclear cells was designed and implemented. Impedance spectroscopy measurements in the range from 1 Hz to 500 MHz were carried out after the osmotic stress of the samples with sodium chloride solution at 1M concentration. The evolution in time after the osmotic stress at two specific frequencies (1.36 kHz and 154 kHz) was analyzed. The device showed its sensitivity to the presence of cells and the evolution of the osmotic processes. Higher values of impedance (around 15% for both the real and imaginary part) were measured at 1.36 kHz in ME/CFS patients compared to control samples. No significant difference was found between patient samples and control samples at 154 kHz. Results help to further understand the diagnosis of ME/CFS patients and the relation of their blood samples with bioimpedance measurements.
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