{"title":"植物不同部位的化学成分估算及抗氧化活性分析","authors":"Pankaj Singh, Rasna Gupta, Shikha Shukla, Ankit Gupta, Ram Lakhan Singh","doi":"10.18231/j.ijpca.2023.032","DOIUrl":null,"url":null,"abstract":"Oxidative stress is defined as a condition in which the balance between the production of reactive oxygen species (ROS) and the antioxidant defense system gets disturbed and causes free radical induced diseases. The current research was aimed to carrying out quantification of phytochemicals in different parts of and explores the antioxidant activities of ethanolic extracts of leaf (CFL), seed (CFS), and bark (CFB) to show their therapeutic importance. The antioxidant potential of plant parts was assessed by free radical scavenging activity (FRSA), superoxide anion radical scavenging activity (SARSA), reducing power (RP), lipid peroxidation (LPO), hydroxyl radical scavenging activity (HRSA), and ferric thiocyanate activity (FTC). Quercetin was used as a standard to measure the antioxidant activity. Among the tested plant extracts, CFB had the highest total phenolics and protein content than those of CFS and CFL, whereas CFL had the highest ascorbic acid, carotenoids and carbohydrate content than CFB and CFS plant parts. Antioxidant assay showed CFB had the lowest ICvalue for FRSA (35.48 μg/ml), LPO (91.91 μg/ml), HRSA (106.87 μg/ml) and FTC (53.21 μg/ml). CFS showed the lowest IC for SARSA (24.54 μg/ml) and the highest RP (2.14 ASE/ml). Overall, the bark of could be a potential natural antioxidant source for food as well as pharmaceutical applications.","PeriodicalId":14182,"journal":{"name":"International Journal of Pharmaceutical Chemistry and Analysis","volume":"49 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Estimation of phytochemical constituents and analysis of antioxidant activity in different parts of plant\",\"authors\":\"Pankaj Singh, Rasna Gupta, Shikha Shukla, Ankit Gupta, Ram Lakhan Singh\",\"doi\":\"10.18231/j.ijpca.2023.032\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Oxidative stress is defined as a condition in which the balance between the production of reactive oxygen species (ROS) and the antioxidant defense system gets disturbed and causes free radical induced diseases. The current research was aimed to carrying out quantification of phytochemicals in different parts of and explores the antioxidant activities of ethanolic extracts of leaf (CFL), seed (CFS), and bark (CFB) to show their therapeutic importance. The antioxidant potential of plant parts was assessed by free radical scavenging activity (FRSA), superoxide anion radical scavenging activity (SARSA), reducing power (RP), lipid peroxidation (LPO), hydroxyl radical scavenging activity (HRSA), and ferric thiocyanate activity (FTC). Quercetin was used as a standard to measure the antioxidant activity. Among the tested plant extracts, CFB had the highest total phenolics and protein content than those of CFS and CFL, whereas CFL had the highest ascorbic acid, carotenoids and carbohydrate content than CFB and CFS plant parts. Antioxidant assay showed CFB had the lowest ICvalue for FRSA (35.48 μg/ml), LPO (91.91 μg/ml), HRSA (106.87 μg/ml) and FTC (53.21 μg/ml). CFS showed the lowest IC for SARSA (24.54 μg/ml) and the highest RP (2.14 ASE/ml). Overall, the bark of could be a potential natural antioxidant source for food as well as pharmaceutical applications.\",\"PeriodicalId\":14182,\"journal\":{\"name\":\"International Journal of Pharmaceutical Chemistry and Analysis\",\"volume\":\"49 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-09-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Pharmaceutical Chemistry and Analysis\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.18231/j.ijpca.2023.032\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Pharmaceutical Chemistry and Analysis","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18231/j.ijpca.2023.032","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Estimation of phytochemical constituents and analysis of antioxidant activity in different parts of plant
Oxidative stress is defined as a condition in which the balance between the production of reactive oxygen species (ROS) and the antioxidant defense system gets disturbed and causes free radical induced diseases. The current research was aimed to carrying out quantification of phytochemicals in different parts of and explores the antioxidant activities of ethanolic extracts of leaf (CFL), seed (CFS), and bark (CFB) to show their therapeutic importance. The antioxidant potential of plant parts was assessed by free radical scavenging activity (FRSA), superoxide anion radical scavenging activity (SARSA), reducing power (RP), lipid peroxidation (LPO), hydroxyl radical scavenging activity (HRSA), and ferric thiocyanate activity (FTC). Quercetin was used as a standard to measure the antioxidant activity. Among the tested plant extracts, CFB had the highest total phenolics and protein content than those of CFS and CFL, whereas CFL had the highest ascorbic acid, carotenoids and carbohydrate content than CFB and CFS plant parts. Antioxidant assay showed CFB had the lowest ICvalue for FRSA (35.48 μg/ml), LPO (91.91 μg/ml), HRSA (106.87 μg/ml) and FTC (53.21 μg/ml). CFS showed the lowest IC for SARSA (24.54 μg/ml) and the highest RP (2.14 ASE/ml). Overall, the bark of could be a potential natural antioxidant source for food as well as pharmaceutical applications.