含有66的Tripartite Motif通过激活基质金属肽酶11促进膀胱癌的恶性进展

IF 2.9 4区 医学 Q1 Medicine Journal of biomedical nanotechnology Pub Date : 2023-09-01 DOI:10.1166/jbn.2023.3663
Jiguang Xiao, Liming Xu, Bo Zheng, Zhun Wu, Jinqu Chen
{"title":"含有66的Tripartite Motif通过激活基质金属肽酶11促进膀胱癌的恶性进展","authors":"Jiguang Xiao, Liming Xu, Bo Zheng, Zhun Wu, Jinqu Chen","doi":"10.1166/jbn.2023.3663","DOIUrl":null,"url":null,"abstract":"We aimed to investigate the pivotal role of Tripartite Motif Containing 66 (TRIM66) in bladder cancer (BCa) and elucidate its underlying mechanism in promoting BCa cell metastasis. Tumor and adjacent normal tissues were collected from 62 BCa patients, and TRIM66 was quantified using quantitative real-time polymerase chain reaction (qRT-PCR). The relationship between TRIM66 expression and clinical indicators, as well as patient prognosis, was analyzed. In addition, an in vitro model was established by silencing TRIM66 in a BCa cell line. The impact of TRIM66 on BCa cell invasion and metastasis was evaluated through Transwell and cell wound healing assays. Through meticulous bioinformatics analysis and luciferase assays, we confirmed that TRIM66 specifically binds to Matrix Metallopeptidase 11 (MMP11). Moreover, mRNA expression analysis revealed a positive correlation between TRIM66 and MMP11 in BCa tumor tissues. Intriguingly, in a cell recovery experiment, overexpression of MMP11 reversed the inhibition of migration and proliferation caused by TRIM66 downregulation. Collectively, our findings unequivocally indicate that heightened TRIM66 expression is closely associated with a malignant phenotype in BCa tissues. Silencing TRIM66 significantly mitigates BCa cell metastasis in vitro by downregulating MMP11. These observations shed light on the critical involvement of the TRIM66-MMP11 axis in BCa progression, offering promising avenues for therapeutic interventions targeting this pathway.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":"68 1","pages":"0"},"PeriodicalIF":2.9000,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Tripartite Motif Containing 66 Promotes Malignant Progression of Bladder Cancer by Activating Matrix Metallopeptidase 11\",\"authors\":\"Jiguang Xiao, Liming Xu, Bo Zheng, Zhun Wu, Jinqu Chen\",\"doi\":\"10.1166/jbn.2023.3663\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"We aimed to investigate the pivotal role of Tripartite Motif Containing 66 (TRIM66) in bladder cancer (BCa) and elucidate its underlying mechanism in promoting BCa cell metastasis. Tumor and adjacent normal tissues were collected from 62 BCa patients, and TRIM66 was quantified using quantitative real-time polymerase chain reaction (qRT-PCR). The relationship between TRIM66 expression and clinical indicators, as well as patient prognosis, was analyzed. In addition, an in vitro model was established by silencing TRIM66 in a BCa cell line. The impact of TRIM66 on BCa cell invasion and metastasis was evaluated through Transwell and cell wound healing assays. Through meticulous bioinformatics analysis and luciferase assays, we confirmed that TRIM66 specifically binds to Matrix Metallopeptidase 11 (MMP11). Moreover, mRNA expression analysis revealed a positive correlation between TRIM66 and MMP11 in BCa tumor tissues. Intriguingly, in a cell recovery experiment, overexpression of MMP11 reversed the inhibition of migration and proliferation caused by TRIM66 downregulation. Collectively, our findings unequivocally indicate that heightened TRIM66 expression is closely associated with a malignant phenotype in BCa tissues. Silencing TRIM66 significantly mitigates BCa cell metastasis in vitro by downregulating MMP11. These observations shed light on the critical involvement of the TRIM66-MMP11 axis in BCa progression, offering promising avenues for therapeutic interventions targeting this pathway.\",\"PeriodicalId\":15260,\"journal\":{\"name\":\"Journal of biomedical nanotechnology\",\"volume\":\"68 1\",\"pages\":\"0\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2023-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of biomedical nanotechnology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1166/jbn.2023.3663\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of biomedical nanotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1166/jbn.2023.3663","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0

摘要

我们旨在研究TRIM66在膀胱癌(BCa)中的关键作用,并阐明其促进BCa细胞转移的潜在机制。采集62例BCa患者的肿瘤及邻近正常组织,采用实时荧光定量聚合酶链反应(qRT-PCR)对TRIM66进行定量检测。分析TRIM66表达与临床指标及患者预后的关系。此外,通过沉默TRIM66在BCa细胞系中建立体外模型。通过Transwell和细胞创面愈合试验评估TRIM66对BCa细胞侵袭和转移的影响。通过细致的生物信息学分析和荧光素酶测定,我们证实TRIM66特异性结合基质金属肽酶11 (MMP11)。此外,mRNA表达分析显示TRIM66与MMP11在BCa肿瘤组织中呈正相关。有趣的是,在细胞恢复实验中,MMP11的过表达逆转了TRIM66下调引起的迁移和增殖抑制。总之,我们的研究结果明确表明,TRIM66的表达升高与BCa组织的恶性表型密切相关。沉默TRIM66可通过下调MMP11显著减轻体外BCa细胞转移。这些观察结果揭示了TRIM66-MMP11轴在BCa进展中的关键作用,为针对这一途径的治疗干预提供了有希望的途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Tripartite Motif Containing 66 Promotes Malignant Progression of Bladder Cancer by Activating Matrix Metallopeptidase 11
We aimed to investigate the pivotal role of Tripartite Motif Containing 66 (TRIM66) in bladder cancer (BCa) and elucidate its underlying mechanism in promoting BCa cell metastasis. Tumor and adjacent normal tissues were collected from 62 BCa patients, and TRIM66 was quantified using quantitative real-time polymerase chain reaction (qRT-PCR). The relationship between TRIM66 expression and clinical indicators, as well as patient prognosis, was analyzed. In addition, an in vitro model was established by silencing TRIM66 in a BCa cell line. The impact of TRIM66 on BCa cell invasion and metastasis was evaluated through Transwell and cell wound healing assays. Through meticulous bioinformatics analysis and luciferase assays, we confirmed that TRIM66 specifically binds to Matrix Metallopeptidase 11 (MMP11). Moreover, mRNA expression analysis revealed a positive correlation between TRIM66 and MMP11 in BCa tumor tissues. Intriguingly, in a cell recovery experiment, overexpression of MMP11 reversed the inhibition of migration and proliferation caused by TRIM66 downregulation. Collectively, our findings unequivocally indicate that heightened TRIM66 expression is closely associated with a malignant phenotype in BCa tissues. Silencing TRIM66 significantly mitigates BCa cell metastasis in vitro by downregulating MMP11. These observations shed light on the critical involvement of the TRIM66-MMP11 axis in BCa progression, offering promising avenues for therapeutic interventions targeting this pathway.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
4.30
自引率
17.20%
发文量
145
审稿时长
2.3 months
期刊介绍: Information not localized
期刊最新文献
Mequinol-Loaded Nano Clay Drug Carriers in a Gelatin Hydrogel for Wound Healing: An Antiinflammatory and Antioxidant Treatment Modality Resveratrol Inhibited Nanoparticles Stromal Interaction Molecule 2 in Regulating miR-20b-5p Signaling Pathway to Improve Mitochondrial Function During Myocardial Ischemia-Reperfusion Injury A Hierarchically Micro- and Nanofibrous Hybrid Hydrogel Derived from Decellularized Skin Matrix with High Bioactivity and Tunable Mechanical Properties for Accelerated Wound Healing Sport Medicine Principles Augment Healing Response in Spinal Cord Injury in a Rat Model Treated with a Curcumin-Loaded Nanocomposite Hydrogel Polyacrylic Acid-Modified Superparamagnetic Iron Oxide Nanoparticles Differentiate Between Hyperplastic and Metastatic Breast Cancer Lymph Nodes
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1