TGFB诱导因子同源盒2通过激活CD2细胞质尾部结合蛋白2诱导膀胱癌恶化

IF 2.9 4区 医学 Q1 Medicine Journal of biomedical nanotechnology Pub Date : 2023-09-01 DOI:10.1166/jbn.2023.3657
Xiaobo Guo, Gang Li, Yufeng Zhao, Bo Zhao
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引用次数: 0

摘要

膀胱癌是一种复杂的侵袭性恶性肿瘤,治疗方案有限。在本研究中,我们旨在探讨TGIF2在膀胱癌中的表达模式及其临床意义,以及其在疾病进展中的功能作用及其与CD2BP2的相互作用。通过定量逆转录聚合酶链反应(qRT-PCR)分析,我们发现与正常膀胱黏膜相比,TGIF2在膀胱癌组织中高表达。此外,升高的TGIF2水平与晚期肿瘤分期和较大肿瘤大小相关,表明其作为膀胱癌预后标志物的潜力。通过对膀胱癌细胞系(253j和J82)的敲低模型,我们观察到TGIF2的抑制导致增殖和迁移率降低,这表明TGIF2在促进这些恶性表型中起着关键作用。此外,我们的双荧光素酶报告试验显示TGIF2和CD2BP2之间存在直接相互作用,CD2BP2在膀胱癌组织中上调,并与TGIF2的表达呈正相关。值得注意的是,CD2BP2的过表达逆转了TGIF2敲低引起的恶性表型抑制。总之,我们的研究结果强调了TGIF2在膀胱癌组织中的丰富表达及其与恶性特征的关联。我们证明TGIF2通过正向调节CD2BP2促进膀胱癌的增殖和转移能力。这些发现为进一步研究TGIF2和CD2BP2作为膀胱癌治疗靶点和预后标志物的潜力提供了基础。
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TGFB Induced Factor Homeobox 2 Induces Deterioration of Bladder Carcinoma via Activating CD2 Cytoplasmic Tail Binding Protein 2
Bladder carcinoma is a complex and aggressive malignancy with limited treatment options. In this study, we aimed to investigate the expression pattern of TGIF2 in bladder carcinoma and its clinical significance, as well as its functional role and interaction with CD2BP2 in disease progression. Through quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis, we found that TGIF2 was highly expressed in bladder carcinoma tissues compared to normal bladder mucosa. Furthermore, elevated TGIF2 levels were associated with advanced tumor stage and larger tumor size, indicating its potential as a prognostic marker in bladder carcinoma. Using knockdown models in bladder carcinoma cell lines (253j and J82), we observed that the inhibition of TGIF2 resulted in decreased proliferation and migration rates, suggesting a critical role of TGIF2 in promoting these malignant phenotypes. Additionally, our dual-luciferase reporter assay revealed a direct interaction between TGIF2 and CD2BP2, with CD2BP2 being upregulated in bladder carcinoma tissues and positively correlated with TGIF2 expression. Notably, the overexpression of CD2BP2 reversed the suppressed malignant phenotypes caused by TGIF2 knockdown. Collectively, our findings highlight the abundant expression of TGIF2 in bladder carcinoma tissues and its association with malignant characteristics. We demonstrate that TGIF2 promotes proliferative and metastatic capacities in bladder carcinoma by positively regulating CD2BP2. These insights provide a basis for further investigations into the potential of TGIF2 and CD2BP2 as therapeutic targets and prognostic markers in bladder carcinoma management.
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来源期刊
CiteScore
4.30
自引率
17.20%
发文量
145
审稿时长
2.3 months
期刊介绍: Information not localized
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