Monika RIEDLE-BAUER, Dragana BANDION, Monika MADERCIC, Markus GORFER
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引用次数: 0
摘要
生物防治剂(bca)对几种与葡萄Esca相关的病原菌有一定的防治效果，但其对地中海白腐菌(Fmed)的防治效果尚未得到广泛的研究。对几种潜在bca的评估评估了对Fmed的活性。其中包括从奥地利葡萄树中分离出的simmoncderma, T. citriviride, T. atroviride，枯草芽孢杆菌，解淀粉芽孢杆菌/velezensis和韩国假单胞菌。bca对Fmed生长的影响通过双重培养试验和新鲜和高压灭菌葡萄藤木片试验进行了评估。在双重培养试验中，与实验对照相比，所有bca都降低了Fmed的生长。在木霉实验中，Fmed的生长仅略微超过初始菌丝塞的大小，对所有Fmed分离株和菌株的生长抑制率为91% ~ 97%。解淀粉芽孢杆菌对Fmed生长的抑制作用为55 ~ 66%，枯草芽孢杆菌对Fmed生长的抑制作用为41 ~ 49%，高丽芽孢杆菌对Fmed生长的抑制作用为55 ~ 66%。在木盘试验中，Fmed定植了新鲜和高压灭菌的木材。所有木霉分离株几乎完全抑制新鲜木材和蒸压木材上的Fmed生长。一株解淀粉芽孢杆菌/velezensis和一株韩国芽孢杆菌的抑制作用虽小，但有统计学意义。
Activity of biocontrol agents against the grapevine pathogen Fomitiporia mediterranea
Biological control agents (BCAs) have shown efficacy against several pathogens associated with Esca of grapevines, but their effects on the white rot pathogen Fomitiporia mediterranea (Fmed) have not been extensively studied. An assessment of several potential BCAs evaluated activity against Fmed. This included isolates of Trichoderma simmonsii, T. citrinoviride, T. atroviride, Bacillus subtilis, B. amyloliquefaciens/velezensis and Pseudomonas koreensis, all obtained from grapevines in Austria. Effects of the BCAs on Fmed growth were assessed in dual culture assays and in assays with fresh and autoclaved grapevine wood disks. In the dual culture assays, all the BCAs reduced growth of Fmed compared to experimental controls. In the Trichoderma experiments, Fmed growth only marginally exceeded the size of the initial mycelium plugs, and growth inhibition for all Fmed isolates and strains was 91 to 97%. Growth of Fmed was inhibited by 55 to 66% by B. amyloliquefaciens/velezensis isolates, by 41 to 49% by B. subtilis isolates, and by 55 to 66% by P. koreensis. In the wood disc assays, Fmed colonized fresh and autoclaved wood. All the Trichoderma isolates almost completely suppressed Fmed growth on fresh and autoclaved wood. Less but statistically significant inhibition was recorded for an isolate of B. amyloliquefaciens/velezensis and one of P. koreensis.
期刊介绍：
Phytopathologia Mediterranea is an international journal edited by the Mediterranean Phytopathological Union. The journal’s mission is the promotion of plant health for Mediterranean crops, climate and regions, safe food production, and the transfer of new knowledge on plant diseases and their sustainable management.
The journal deals with all areas of plant pathology, including etiology, epidemiology, disease control, biochemical and physiological aspects, and utilization of molecular technologies. All types of plant pathogens are covered, including fungi, oomycetes, nematodes, protozoa, bacteria, phytoplasmas, viruses, and viroids. The journal also gives a special attention to research on mycotoxins, biological and integrated management of plant diseases, and the use of natural substances in disease and weed control. The journal focuses on pathology of Mediterranean crops grown throughout the world.
The Editorial Board of Phytopathologia Mediterranea has recently been reorganised, under two Editors-in-Chief and with an increased number of editors.
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