Investigation on growth modeling and kinetic analysis of lead-resistant bacteria Bacillus infantis isolated from battery industry waste mud

The present investigation introduces a novel approach on advanced biological remediation for heavy metal lead removal from industrial waste. The ongoing research collaborating theoretical insights with experimentally-derived data to develop strategies to operate bioreactor proficiently on large scale mode. Initially bacteria naturally well equipped with lead resistance has been isolated and identified from native source 16srDNA study along with other microbiological tests have been conducted with the isolated lead resistant bacterial cell and it has been revealed that the isolated lead resistant cell is Bacillus infantis 4352-1T. An extensive studies regarding cell growth of the candidate cell has been carried out in batch mode using well plugged 100 ml conical flasks placed on a rotary shaker under aseptic condition to determine the activity of Bacillus infantis 4352-1T towards its lead removal.Then an attempt has been made to set out to formulate an equation that characterizes the growth kinetics of the lead-resistant Bacillus infantis 4352-1T under lead microenvironment. Through a combination of theoretical analysis and experimental data, it has been observed that Monod’s equation accurately describes the cell growth progress within concentration range of lead (0.05-0.25 kg lead/m 3 ). In this range a theoretical analysis with the help of experimental data is not only confirmed the validity of Monods equation within the concentration range (0.05-0.25 kg lead/m 3 ) but also helped to derive the maximum specific cell growth rate 0.0237 hr −1 and substrate saturation constant 0.018 kg/m 3 . Interestingly, further experiments has been conducted with lead concentrations beyond 0.25 kg lead/m 3 and upto 0.43 kg lead/m 3 show a swift declining in the specific cell growth rate signifying pronounced effect of substrate inhibition clarified through Haldane equation quantitatively.