Application of CRISPRCas9 in Gene Editing of <i>Aedes aegypti</i> Mosquitoes: Methods and Challenges

This review explores the application of CRISPR/Cas9 technology in gene editing in the Aedes aegypti  mosquito, focusing on its methods and challenges. This review describes the importance of the Aedes aegypti  mosquito, particularly its association with infectious diseases such as dengue fever, Zika virus and malaria, and explains in detail the basic principles of the CRISPR/Cas9 technology, how it compares with traditional gene editing methods and its potential in mosquito gene editing. This review highlights previous studies using CRISPR/Cas9 in the Aedes aegypti  mosquito, emphasizing the potential applications and challenges of the technology. Ethical considerations, technical limitations and the impact of gene prevalence are also discussed in a comprehensive manner. This review provides recommendations to address ethical and ecological issues and emphasizes the exploration of future research and applications. This review provides strong support for the use of CRISPR/Cas9 technology to improve the control of mosquito-borne infectious diseases, while also emphasizing the need to address ethical and ecological risk issues.