Establishment and Optimization of an in vitro Regeneration System in Shredded Pineapple

Shredded pineapple, a type of rare high-quality fresh pineapple, is convenient to eat and has high economic value worldwide. In this study, the crown buds and sucker buds of shredded pineapple were used to study the effects of different explants and the ratios of different hormones (i.e., 6-benzylaminopurine, 6-BA; naphthaleneacetic acid, NAA; and indolebutyric acid, IBA) on the induction, proliferation, differentiation, and rooting of pineapple, and the effects of seedling refining on the survival rate of transplants, to establish a regeneration system for shredded pineapple. The results showed that the most suitable explant was crown buds, the optimal medium for adventitious bud induction was MS+3.0 mg/L 6-BA+3.0 mg/L NAA, the optimal proliferation medium was MS+3.0 mg/L 6-BA+1.0 mg/L NAA, the optimal adventitious bud growth was the MS medium, and the optimal rooting medium was MS+2.0 mg/L IBA. The peak survival rate of regenerated plants was observed after seven days of seedling refining. This study successfully established and optimized a tissue culture and rapid propagation regeneration system for shredded pineapple, which provides technical support for the low-cost rapid production and genetic transformation of pineapples.