{"title":"非靶向代谢组学分析揭示了不同苹果群体中多酚的生化变化","authors":"Jun Song, Beatrice Amyotte, Cindy H.J. Yu, Leslie Campbell Palmer, Melinda Vinqvist-Tymchuk, H.P. Vasantha Rupasinghe","doi":"10.48130/frures-2023-0029","DOIUrl":null,"url":null,"abstract":"Apples are an important dietary source of polyphenols as antioxidants that contribute to human health. Based on total phenolic content and total antioxidant capacity data gathered from a large apple biodiversity collection, we applied untargeted metabolomics analyses to further understand the phenolic composition in select accessions with very low and very high phenolic contents, as well as in commercial cultivars. The liquid chromatography-mass spectrometry (LC-MS) analysis provided both qualitative and quantitative information, with 2,946 features detected using the positive mode of electrospray ionization. We found significant variation in total polyphenols, and strong correlations between total phenolic content and total antioxidant capacity. Polyphenolic compounds significantly associated with each group of apples were quantified, and new features were putatively identified. This study provides new knowledge regarding the specific polyphenols that contribute to the variation in total phenolic content and antioxidant capacity among apples, and new insights into the biochemical regulations of polyphenol biosynthesis and composition.","PeriodicalId":37667,"journal":{"name":"Fruit Growing Research","volume":"27 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Untargeted metabolomics analysis reveals the biochemical variations of polyphenols in a diverse apple population\",\"authors\":\"Jun Song, Beatrice Amyotte, Cindy H.J. Yu, Leslie Campbell Palmer, Melinda Vinqvist-Tymchuk, H.P. Vasantha Rupasinghe\",\"doi\":\"10.48130/frures-2023-0029\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Apples are an important dietary source of polyphenols as antioxidants that contribute to human health. Based on total phenolic content and total antioxidant capacity data gathered from a large apple biodiversity collection, we applied untargeted metabolomics analyses to further understand the phenolic composition in select accessions with very low and very high phenolic contents, as well as in commercial cultivars. The liquid chromatography-mass spectrometry (LC-MS) analysis provided both qualitative and quantitative information, with 2,946 features detected using the positive mode of electrospray ionization. We found significant variation in total polyphenols, and strong correlations between total phenolic content and total antioxidant capacity. Polyphenolic compounds significantly associated with each group of apples were quantified, and new features were putatively identified. This study provides new knowledge regarding the specific polyphenols that contribute to the variation in total phenolic content and antioxidant capacity among apples, and new insights into the biochemical regulations of polyphenol biosynthesis and composition.\",\"PeriodicalId\":37667,\"journal\":{\"name\":\"Fruit Growing Research\",\"volume\":\"27 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Fruit Growing Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.48130/frures-2023-0029\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fruit Growing Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.48130/frures-2023-0029","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
Untargeted metabolomics analysis reveals the biochemical variations of polyphenols in a diverse apple population
Apples are an important dietary source of polyphenols as antioxidants that contribute to human health. Based on total phenolic content and total antioxidant capacity data gathered from a large apple biodiversity collection, we applied untargeted metabolomics analyses to further understand the phenolic composition in select accessions with very low and very high phenolic contents, as well as in commercial cultivars. The liquid chromatography-mass spectrometry (LC-MS) analysis provided both qualitative and quantitative information, with 2,946 features detected using the positive mode of electrospray ionization. We found significant variation in total polyphenols, and strong correlations between total phenolic content and total antioxidant capacity. Polyphenolic compounds significantly associated with each group of apples were quantified, and new features were putatively identified. This study provides new knowledge regarding the specific polyphenols that contribute to the variation in total phenolic content and antioxidant capacity among apples, and new insights into the biochemical regulations of polyphenol biosynthesis and composition.
期刊介绍:
FRUIT GROWING RESEARCH is an international journal, which has been issued under the care of the Research Institute for Fruit Growing Pitesti, Romania. Fruit growing research is an annual journal aimed at disseminating significant research and original papers on genetic resources, breeding of deciduous fruit trees species; fruit sustainable technologies – environment protection; propagation, virology and tissue culture, postharvest, that can be of interest to a wide audience of plant scientists in all areas of fruit growing. An international Editorial Board advises the journal.