分裂定时AI肉牛的发情反应贴片、人工授精时机和GnRH方案

Danilo Demeterco, Ryon S. Walker, Luiz Ernandes Kozicki, Vitor Mohad Valle, A.K. Edwards, Jake E. Anderson, Cathleen Collet Williams
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摘要

在7天co - sync +CIDR协议下,采用发情检测补丁确定STAI的最佳时机和在STAI时GnRH的必要性。将216头杂交肉牛分为以下处理组:去除CIDR后72 h,按CTRL=TAI (n=67)处理;去除CIDR后72、84 h,按TRT=STAI (n=149)处理。所有雌性小鼠在第10天接受GnRH (100 mcg)加CIDR,在第7天接受PGF2α,去除CIDR和estroprotect发情检测器贴片。在cidr移除72 h后,分配补丁评分(PS1<50%移除;PS2>50%删除)所有女性。对照组奶牛在TAI 72 h时再次注射GnRH (100 mcg)。PS2的TRT组奶牛不给GnRH。84 h,其余TRT组奶牛给予第二次PS;PS1奶牛注射GnRH (100 mcg), PS2奶牛不注射GnRH。结果:对照组(45.6%)与对照组(44.8%)TAI妊娠率差异无统计学意义(P=0.81)。PS2奶牛的妊娠率(50.3%)高于PS1奶牛(29.4%)(P=0.07)。然而,通过将无反应奶牛的TAI延长至84 h, 82.0%的TRT奶牛在TAI时未接受第二次GnRH注射。综上所述,发情检测贴片在不影响妊娠率的情况下降低了妊娠期奶牛GnRH需要量的百分比。发情检测补丁显著减少了在TAI接受第二次GnRH注射的奶牛数量。
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Estrus response patches, timing for artificial insemination, and GnRH protocol in Split Timed AI beef cattle
Abstract An estrous-detection patch was used to determine the optimum timing for STAI and the necessity of GnRH at STAI on a 7-day CO-Synch+CIDR protocol. Crossbred beef cows (n=216) were stratified into the following treatment groups: CTRL=TAI (n=67) at 72 h post CIDR removal, or TRT=STAI (n=149) at 72 or 84 h post CIDR removal. All females received GnRH (100 mcg) plus a CIDR on d0, PGF2α, CIDR removal, and an Estrotect estrous-detector patch on d7. At 72 h post-CIDR removal, a patch score was assigned (PS1<50% removed; PS2>50% removed) to all females. Cows in the CTRL group were administered a second GnRH (100 mcg) at 72 h TAI. Cows in the TRT group with PS2 were not administered GnRH. At 84 h, the remaining TRT group cows were given a second PS; cows with PS1 received a GnRH (100 mcg), and cows with PS2 were not administered. Results: The TAI pregnancy rates were similar (P=0.81) between the CTRL (45.6%) and TRT (44.8%) groups. Pregnancy rates tended to be higher (P=0.07) for cows with PS2 (50.3%) than for those with PS1 (29.4%). However, by extending TAI to 84 h in unresponsive cows, 82.0% of TRT cows did not receive a second injection of GnRH at TAI. It was concluded that the estrous detector patches reduced the percentage of cows that required GnRH at TAI without compromising pregnancy rates. The estrous detector patches significantly reduced the number of cows that received a second GnRH injection at TAI.
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