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Radiography, Ultrasound, and Anthropometry in Dog Nutrition Evaluation 狗狗营养评估中的射线照相术、超声波和人体测量法
Pub Date : 2023-09-22 DOI: 10.1590/1809-6891v24e-75686E
Ricardo de Souza Buzo, Jardel Felipe Tremea, Guilherme Andraus Bispo, Bruna Silva Oliveira, Jaqueline Bizi, Wagner Luis Ferreira, Luciana Del Rio Pinoti
Abstract The nutritional status of dogs is a prominent theme nowadays because it is considered one of the five vital parameters of animals. There are several techniques that allow diagnosing disorders or disturbances in the body condition of these patients. Although it has some subjectivity, as it is a visual and palpation evaluation, the body condition score (BCS) is the most used for this purpose. The present study evaluated radiographic, sonographic and anthropometric measurements of subcutaneous fat deposited in the dorsal region over the seventh lumbar vertebra of 100 dogs, compared to a double-blind classification of patients on the numerical scale of the BMC. There was a positive correlation of the sonographic measurements with the body condition score (p-value below 2 x 10-16 and R = 0.54) and the same was observed with the radiographic measurements (p-value below 2 x 10-16 and R = 0.56) and anthropometric evaluations (whose p-value ranged from 1.55 x 10-7 to 2.34 x 10-11 and its R was 0.24 to 0.36). With this, it was possible to identify greater intensity in the relationship of imaging exams with ECC. It is concluded that radiography and ultrasonography have great potential for use in clinical routine as a more accurate and affordable way to diagnose nutritional disorders. This is the first study to use such equipment together with imaging tests to contribute to the early diagnosis of changes in nutritional status and promote improvements in the follow-up of weight gain or weight loss programs.
摘要 狗的营养状况是当今一个突出的主题,因为它被认为是动物的五个重要参数之一。有几种技术可以诊断这些患者身体状况的紊乱或失调。身体状况评分(BCS)是最常用的诊断方法,但由于它是通过视觉和触诊进行评估,因此具有一定的主观性。本研究对 100 只狗第七腰椎背侧沉积的皮下脂肪进行了放射学、声波图和人体测量学测量,并与根据 BMC 数字量表对患者进行的双盲分类进行了比较。超声波测量结果与身体状况评分呈正相关(p 值低于 2 x 10-16,R = 0.54),与放射学测量结果(p 值低于 2 x 10-16,R = 0.56)和人体测量评估结果(p 值在 1.55 x 10-7 到 2.34 x 10-11 之间,R 值在 0.24 到 0.36 之间)也呈正相关。由此可见,影像学检查与 ECC 的关系更为密切。结论是,放射成像和超声波成像作为一种更准确、更经济的诊断营养失调的方法,在临床常规应用中具有很大的潜力。这是第一项将此类设备与成像检查结合使用的研究,有助于早期诊断营养状况的变化,并促进改善体重增加或体重减轻计划的后续工作。
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引用次数: 0
Effects of maternal nutrition in the final third of gestation on performance and body composition of progeny at slaughter 妊娠期最后三分之一的母体营养对后代屠宰时的表现和身体成分的影响
Pub Date : 2023-07-10 DOI: 10.1590/1809-6891v24e-74730E
J. Klein, Sander Martinho Adams, Dari Celestino Alves, Ivan Luiz Brondani, Luiz Ângelo Damian Pizutti, J. M. Cocco
This study aimed to assess how maternal nutrition in the final third of gestation affects progeny weight performance and body composition. Forty-one steers were included, born to cows subjected to different nutritional levels during this period: 13 cows without supplementation and under nutritional restriction (RES), 16 cows supplemented to meet 100% of requirements (REQ), and 12 cows supplemented to meet 150% of requirements (HIGH). The study design was completely randomized. Progeny performance was not influenced by maternal nutrition during gestation (P > 0.05). However, RES animals excelled during challenging periods, while REQ and HIGH animals performed better in nutritionally favorable environments. Maternal nutrition in the final third of gestation did not impact the contribution of non-carcass components (16.42%) and internal organs (3.17%). RES and HIGH steers had a higher relative weight of the rumen (2.48%) compared to REQ steers (2.24%), resulting in a greater proportion of the gastrointestinal tract (8.25% vs. 7.63%). Carcass characteristics did not differ significantly between treatments (P > 0.05), with an average hot carcass weight and yield of 304.28 kg and 57.80%, respectively. The primary fore, side, and hind cuts represented 39.22%, 10.64%, and 50.67% of the carcass, respectively. Overall, maternal nutrition during gestation affects fetal development, leading to modifications in body composition and, consequently, the productive potential of the offspring.
本研究旨在评估母体在妊娠期最后三分之一的营养如何影响后代的体重表现和身体成分。研究对象包括 41 头母牛所产的小公牛,这些母牛在此期间接受了不同营养水平的饲养:13头母牛未补充营养,处于营养限制状态(RES);16头母牛补充营养,以满足100%的需求(REQ);12头母牛补充营养,以满足150%的需求(HIGH)。研究设计完全随机。后代的表现不受妊娠期母体营养的影响(P > 0.05)。然而,RES动物在挑战期表现优异,而REQ和HIGH动物在营养有利的环境中表现更好。妊娠期最后三分之一的母体营养对非胴体成分(16.42%)和内脏器官(3.17%)的贡献率没有影响。与 REQ 牛(2.24%)相比,RES 和 HIGH 牛的瘤胃相对重量更高(2.48%),这导致胃肠道所占比例更大(8.25% 对 7.63%)。不同处理之间的胴体特征没有显著差异(P > 0.05),平均热胴体重量和产量分别为 304.28 千克和 57.80%。主要的前、侧和后切肉分别占胴体的 39.22%、10.64% 和 50.67%。总之,妊娠期间母体的营养会影响胎儿的发育,导致身体成分的改变,进而影响后代的生产潜力。
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引用次数: 0
Effect of replacing corn with cactus pear on the performance and carcass traits and meat quality of feedlot finished lambs 用仙人掌梨替代玉米对饲养场成品羔羊的性能、胴体特征和肉质的影响
Pub Date : 2023-07-10 DOI: 10.1590/1809-6891v24e-75322E
Karina de Alencar Alves, Joana Angélica Matias de Lima, Marcus Roberto Góes Ferreira Costa, Thayná Campos da Silva, Cicero de Lima Brito, Maria Clementina Rodrigues Gomes, José Morais Pereira, Juliana P F Oliveira, R. R. Nascimento, L. Bezerra
Abstract The aim was to evaluate the effect of replacing ground corn with spineless cactus from two species Orelha de Elefante Mexicana-OEM (Opuntia stricta Haw.) and Gigante (Opuntia ficus-indica Mill) in a total mixed ration for finishing lambs evaluating its effects on intake, ingestive behavior, performance, carcass traits, commercial cuts and physiochemical composition of the meat. Eighteen crossbred lambs with average body weight of 15.0 ± 2.32 kg were distributed in a completely randomized design with three treatments (OEM and Gigante spineless cactus and ground corn as control treatment) and six replications. Spineless cactus species replacing ground corn in lambs diets does not change the intake of dry matter (DMI), crude protein and neutral detergent fiber or time (min/d) of ingestion, rumination and idleness, or final body weight gain. Lambs fed with ground corn and "Gigante" cactus presented a higher total weigh gain (TWG) and average daily weight gain (ADG) when compared to OEM spineless specie. Ground corn diet promoted better hot and cold carcass yield in lambs receiving spineless cactus, regardless of species. Feed and total costs (kg/lamb) were higher for the ground corn diet. The replacement of ground corn with spineless cactus did not change moisture, protein, and ash meat contents, as well as water holding capacity, cooking loss, shear force, and a* color intensity. However, there was an effect for the meat lipid content, b* and L* color intensity and color index, where the species of cactus Gigante on ground corn presented the highest lipid content and yellowness (b*) intensity and lower L* color compared to OEM. Spineless cactus species Gigante can replace ground corn as a source of energy in diets for finishing lambs because it significantly improves the financial income for the producer without changing the ADG, DMI, ingestive behavior and yield of commercial cuts.
摘要 目的是评估在育成羔羊的全混合日粮中用两种无刺仙人掌:Orelha de Elefante Mexicana-OEM(Opuntia stricta Haw.)和 Gigante(Opuntia ficus-indica Mill)替代碎玉米的效果,评估其对采食量、摄食行为、生产性能、胴体特征、商品肉和肉的理化成分的影响。18 只杂交羔羊的平均体重为 15.0 ± 2.32 千克,采用完全随机设计,三个处理(OEM 和 Gigante 无刺仙人掌,磨碎的玉米作为对照处理),六个重复。在羔羊日粮中用无刺仙人掌替代碎玉米不会改变干物质(DMI)、粗蛋白和中性洗涤纤维的摄入量或摄入时间(分钟/天)、反刍和怠速以及最终体重增加。与 OEM 无刺品种相比,饲喂磨碎玉米和 "Gigante "仙人掌的羔羊总增重(TWG)和平均日增重(ADG)更高。无论羔羊的品种如何,玉米粉日粮都能提高无刺仙人掌羔羊的冷热胴体产量。碎玉米日粮的饲料成本和总成本(公斤/羔羊)较高。用无刺仙人掌代替碎玉米并不会改变水分、蛋白质和灰分含量,也不会改变持水量、蒸煮损失、剪切力和 a* 色度。但对肉脂含量、b*和 L*色度及颜色指数有影响,与 OEM 相比,在碎玉米上添加仙人掌品种 Gigante 的肉脂含量和黄度(b*)强度最高,L*色度较低。无刺仙人掌品种 Gigante 可替代碎玉米作为育成羔羊日粮的能量来源,因为它能显著提高生产者的经济收入,而不会改变商品羔羊的 ADG、DMI、采食行为和产量。
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引用次数: 0
Resistance of gastrointestinal nematoids of goats and sheep to the antihelmintics levamisol, ivermectin and albendazole 山羊和绵羊胃肠道线虫对抗虫药左旋咪唑、伊维菌素和阿苯达唑的抗药性
Pub Date : 2023-07-10 DOI: 10.1590/1809-6891v24e-75316E
Swênia Christina Pinheiro Soares, Arnon Cunha Reis, Rogério Lean Pereira Castro, Pedro Celestino Serejo Pires, Celecina Saraiva Martins Cabral, Durval Oliveira Diniz, Camila Barros Costa, Dara Maria da Costa Pinheiro, Yasmin Suelen Alves Pinheiro, Nayla Helena Silva Buna, L. Costa, Danilo Rodrigues Barros Brito
Abstract The aim of this study was to determine the resistance of gastrointestinal nematodes in goats and sheep to the anthelmintic drugs levamisole, ivermectin, and albendazole in the metropolitan region of São Luís Island, Maranhão, Brazil. Fecal samples were collected from 150 animals across four different farms; two farms had goats, and the other two had sheep. The samples were then randomly divided into three to four groups of 10 animals: Group I: control, without treatment; Group II: ivermectin treatment; Group III: levamisole treatment; and Group IV: albendazole treatment. Stool samples were collected from the rectal ampulla one day before treatment and 10 days after anthelmintic treatment. Individual coproparasitological examinations were performed using the modified McMaster technique at the Animal Health Laboratory of the Federal Institute of Maranhão, Campus São Luís-Maracanã. The efficacies of the anthelmintic drugs against gastrointestinal nematodes in goats and sheep were: 14.28%, and 13.6% for ivermectin; 0% and 79.4% for levamisole; and 59.8% and 3.43% for albendazole, respectively Gastrointestinal nematodes demonstrated multiple anthelmintic resistance, as the percentage reduction in egg count was less than 95% and the lower limit of the confidence interval was less than 90%. Graphical summary
摘要 本研究旨在确定巴西马拉尼昂州圣路易斯岛大都会地区山羊和绵羊胃肠道线虫对抗虫药左旋咪唑、伊维菌素和阿苯达唑的耐药性。从四个不同农场的 150 只动物身上采集了粪便样本;其中两个农场饲养山羊,另外两个农场饲养绵羊。然后将样本随机分为三至四组,每组 10 头动物:第一组:对照组,不进行治疗;第二组:伊维菌素治疗组;第三组:左旋咪唑治疗组;第四组:阿苯达唑治疗组。分别在治疗前一天和治疗后 10 天从直肠肛门采集粪便样本。马拉尼昂联邦学院圣路易斯-马拉卡纳校区动物健康实验室采用改良的麦克马斯特技术对每只动物进行了共寄生虫学检查。抗蠕虫药物对山羊和绵羊胃肠道线虫的有效率分别为 14.28% 和 13.28%:伊维菌素的有效率分别为 14.28% 和 13.6%;左旋咪唑的有效率分别为 0% 和 79.4%;阿苯达唑的有效率分别为 59.8% 和 3.43% 胃肠道线虫对多种抗线虫药物具有抗药性,因为虫卵数量减少的百分比低于 95%,置信区间的下限低于 90%。图表总结
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引用次数: 0
Occurrence of Cryptosporidium in Anglonubian goats in the municipality of Teresina, state of Piauí, Brazil 巴西皮奥伊州特雷西纳市安格鲁努比亚山羊中隐孢子虫的发生率
Pub Date : 2023-07-10 DOI: 10.1590/1809-6891v24e-75154E
Marcelo Richelly Alves de Oliveira, Carlos Syllas Monteiro Luz, A. F. Evangelista, Geandro Carvalho Castro, Katia Ds Bresciani, K. R. D. Santos, Severino Cavalcante de Sousa
Abstract This research aimed to investigate the occurrence of Cryptosporidium and correlate it with types of housing, feces consistency, and physiological parameters related to the reproductive status of Anglo-Nubian goats reared in the State of Piauí, Brazil. A total of 180 fecal samples were collected from 60 non-pregnant and lactating goats with a mean weight of 35 kg, a body condition score of 3.5, and a mean age of three years from an experimental herd at the Federal University of Piauí (UFPI). Oocysts of protozoa of the genus Cryptosporidium could be found in the studied animals using the modified Ziehl-Neelsen technique in fecal smears and the image analysis system to perform morphometry. Each independent variable in the quantitative and qualitative analyses, that is, weight, body condition score (BCS), physiological status (non-pregnant or lactating), feces consistency (normal, pasty, or diarrheal), and floor types (concrete and slatted), was tested with the dependent variable (positive samples, i.e., the presence of Cryptosporidium oocysts). Twenty-four out of the total number of fecal samples were considered positive for the presence of the protozoan, which means that 13.3% of the animals were parasitized. Moreover, 100% of the positive feces samples had normal consistency (firm) and all parasitized animals were reared in pens with a concrete floor. A statistical variation was observed in the BCS of parasitized animals compared to non-parasitized ones (p > 0.0253). The results showed that the occurrence of Cryptosporidium in experimental goats located in the municipality of Teresina, State of Piauí, Brazil, was considered low, requiring sanitary management measures to prevent infection in animals and humans. This is the first report of Cryptosporidium infection in goats in the State of Piauí.
摘要 本研究旨在调查隐孢子虫的发生情况,并将其与巴西皮奥伊州饲养的盎格鲁-努比亚山羊的饲养类型、粪便浓度以及与繁殖状况相关的生理参数联系起来。研究人员从皮奥伊联邦大学(UFPI)实验牧群中的 60 只非怀孕和哺乳期山羊身上采集了 180 份粪便样本,这些山羊的平均体重为 35 千克,体况评分为 3.5,平均年龄为 3 岁。使用改良的齐氏-奈尔森技术在粪便涂片中发现了隐孢子虫属原生动物的卵囊,并使用图像分析系统进行了形态测量。定量和定性分析中的每个自变量,即体重、体况评分(BCS)、生理状态(未怀孕或哺乳)、粪便稠度(正常、糊状或腹泻)和地板类型(水泥和板条),都与因变量(阳性样本,即存在隐孢子虫卵囊)进行了测试。在所有粪便样本中,有 24 份样本的隐孢子虫卵囊含量呈阳性,这意味着有 13.3% 的动物体内有隐孢子虫寄生。此外,100% 的阳性粪便样本浓度正常(坚实),而且所有寄生动物都饲养在铺有水泥地面的圈舍中。与未寄生的动物相比,寄生动物的 BCS 存在统计学差异(P > 0.0253)。结果表明,隐孢子虫在巴西皮奥伊州特雷西纳市实验山羊中的发生率较低,需要采取卫生管理措施以防止动物和人类感染隐孢子虫。这是皮奥伊州首次报告山羊感染隐孢子虫。
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引用次数: 0
Avaliação ex vivo da coloração da cápsula anterior da lente de cavalos com três concentrações de violeta genciana para treinamento cirúrgico 用三种浓度龙胆紫进行手术训练的马晶状体前囊染色的体外评价
Pub Date : 2023-01-01 DOI: 10.1590/1809-6891v24e-76196p
Marcelle Bettio, Maiara Poersch Seibel, Maria Eduarda Mattos Franceschini, Rafaella Silva Rocha, Renata Lima Baptista, Anita Marchionatti Pigatto, João Antonio Tadeu Pigatto
Resumo O objetivo deste estudo foi avaliar e comparar a eficácia de três concentrações de violeta genciana (0,5%, 0,1% e 0,05%) na coloração da cápsula anterior da lente em equinos. Trinta e seis olhos de equinos post-morten foram utilizados. De acordo com a concentração de violeta genciana utilizada, os olhos foram subdivididos em três grupos compostos por 12 olhos cada. A avaliação da eficácia em coloração da cápsula anterior da lente com diferentes concentrações de violeta de genciana foi realizada por meio de um sistema empírico de avaliação da coloração adequada ou inadequada de retalhos capsulares. Com base na avaliação dos examinadores, as concentrações de 0,1% e 0,05% de violeta de genciana permitiram a visualização adequada da cápsula anterior para o treinamento da capsulotomia curvilínea contínua enquanto a concentração de 0,5% produziu uma coloração capsular forte e inadequada. O modelo desenvolvido com violeta genciana, nas concentrações de 0,1% e 0,05%, permitiu a visualização nítida do retalho capsular, o que o torna viável como modelo para treinamento da etapa de capsulotomia curvilínea contínua em cirurgia de catarata em equinos.
摘要本研究的目的是评价和比较三种浓度龙胆紫(0.5%、0.1%和0.05%)对马晶状体前囊染色的效果。36只死后马的眼睛被使用。根据龙胆紫的浓度,将眼睛分为三组,每组12只眼睛。通过一个经验系统来评估不同浓度龙胆紫对晶状体前囊染色的有效性,以评估胶囊皮瓣染色的适当或不适当。根据检验员的评估,0.1%和0.05%的龙胆紫浓度可以充分观察前囊,以训练连续弯曲的囊切开术,而0.5%的浓度产生强烈和不充分的囊染色。用0.1%和0.05%浓度的龙胆紫建立的模型可以清晰地看到包膜瓣,这使得它可以作为训练马白内障手术中连续曲线包膜切开术步骤的模型。
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引用次数: 0
Função discriminatória de lógica Fuzzy para avaliação de cabras expostas a ocorrência de verminose quanto à resistência, resiliência ou sensibilidade ao parasitismo 模糊逻辑鉴别函数评价山羊暴露于蠕虫的抗性、恢复力或对寄生虫的敏感性
Pub Date : 2023-01-01 DOI: 10.1590/1809-6891v24e-74727p
Wellhington Paulo da Silva Oliveira, Natanael Pereira da Silva Santos, Max Brandão de Oliveira, Amauri Felipe Evangelista, Raimundo Tomaz da Costa Filho, Adriana Mello de Araújo
Resumo A incidência de verminose é um dos principais obstáculos para a caprinocultura nos trópicos. A variação individual da resposta do animal à enfermidade existe, mas precisa ser determinado o seu componente genético e estabelecer o manejo zootécnico dos rebanhos, priorizando a seleção de animais mais resistente ao parasitismo. Objetivou-se nesse estudo avaliar a resposta de cabras à incidência de verminose sob condições de infecção natural a campo, com informações de ovos por grama de fezes (OPG), escore da condição corporal (ECC) e grau de coloração da mucosa conjuntiva (FAMACHA©), recorrendo a utilização de análise de agrupamento e a aplicação de inteligência artificial (IA). Foram utilizadas 3.839 informações de 200 indivíduos em um rebanho experimental de caprinos no Piauí. Considerou-se como resposta ao parasitismo a expressão fenotípica de resistência, sensibilidade e resiliência a verminose, submetidos a três métodos de agrupamento: Ward, Average e K-means, comparado com a lógica Fuzzy, obtidos com o software web CAPRIOVI. Os resultados demonstraram que os grupos de animais resistente, resiliente e sensível ao parasitismo foram estatisticamente distintos (P<0,05). As cabras durante a gestação e o periparto foram identificadas como fases de maior sensibilidade ao parasitismo (P<0,05). O CAPRIOVI aplica a lógica Fuzzy e apresentou o menor percentual de acerto global (77,00%), enquanto os métodos estatísticos tradicionais se destacaram com percentual de acerto global superior a 90,00%, demonstrando excelência estatística com esse fim. Os métodos de agrupamentos apresentaram semelhança na eficiência, mas diferiram quanto à distribuição de animais por agrupamento, com tendência de maior quantidade na categoria resistente. A aplicação da lógica Fuzzy contornou essa limitação ao direcionar a formação dos grupos visando atender o interesse do produtor, inserindo consistência em termos de resposta dos animais a verminose, qualificando o software com potencial para adequação ao manejo sanitário de caprinos.
寄生虫病的发病率是热带地区山羊养殖的主要障碍之一。动物对疾病反应的个体差异是存在的,但需要确定其遗传成分,并建立畜群的动物技术管理,优先选择更抗寄生虫的动物。安装了代表性评价答案山羊得了条件下的自然感染的发病率在球场上,鸡蛋每克粪便信息(OPG),得分的身体状况(ECC)和粘膜染色程度结膜(FAMACHA©),而是使用分组和分析应用了人工智能(ai)。在piaui的一个实验山羊群中,使用了200个个体的3839条信息。它被认为是对寄生虫的抗性、敏感性和恢复力的表型表达,提交到三种聚类方法:Ward、Average和K-means,并与CAPRIOVI web软件获得的模糊逻辑进行比较。结果表明,抗寄生虫组、抗寄生虫组和对寄生虫敏感组差异有统计学意义(p < 0.05)。山羊在妊娠期和围产期被确定为对寄生最敏感的阶段(p < 0.05)。CAPRIOVI应用模糊逻辑,总体命中率最低(77.00%),而传统统计方法的总体命中率高于90.00%,在这方面表现出统计学上的卓越。聚类方法的效率相似,但聚类动物分布不同,抗性类动物数量较多。模糊逻辑的应用绕过了这一限制,指导群体的形成,以满足生产者的利益,在动物对蠕虫的反应方面插入一致性,使软件具有适应山羊健康管理的潜力。
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引用次数: 0
Técnicas de enriquecimento ambiental para gambás-de-orelha-preta (Didelphis aurita Wied-Neuwied, 1826) em cativeiro 圈养黑耳负鼠(Didelphis aurita Wied-Neuwied, 1826)的环境富集技术
Pub Date : 2023-01-01 DOI: 10.1590/1809-6891v24e-76165p
Carlos Eduardo de Noronha, Rosana Suemi Tokumaru
Resumo O objetivo deste trabalho foi analisar os efeitos do enriquecimento ambiental alimentar no comportamento dos gambás-de-orelha-preta que se encontravam em cativeiro no Centro de Triagem de Animais Silvestres (CETAS), no município Serra, no estado do Espírito Santo (ES), de agosto a outubro de 2022. Dois modelos alimentares foram construídos para o estudo: os “Tubos Surpresa” e o “Quebra-Cabeça Alimentar”. Foram selecionados 24 gambás-de-orelha-preta, independente do sexo, juvenis, divididos em oito grupos, com três animais cada, sendo quatro grupos expostos a um modelo e quatro ao outro. Cada grupo foi submetido a duas condições: experimental, com a presença do modelo e controle, sem a presença do modelo. Cada condição durou 24 horas e ocorreram em dois dias consecutivos. Foram filmadas com câmera trap, resultando em 3233 vídeos, de 25 segundos cada. Para elaboração do etograma foram selecionados 24 vídeos de cada grupo, gravados entre as 18h00 e 19h30hs, período em que os animais se mostraram mais ativos. Os gambás interagiram com os dois modelos, acessando e comendo os alimentos escondidos, havendo preferência pela carne em comparação com a fruta. Não houve diferença significativa no comportamento dos gambás em relação aos modelos e observou-se que o comportamento agressivo diminuiu significativamente quando os modelos estavam presentes. Conclui-se que os modelos podem ser utilizados como enriquecimento ambiental para os gambás-de-orelha-preta, trazendo benefícios para a diminuição do comportamento agressivo.
摘要本研究的目的是分析食物环境富集对2022年8月至10月在espirito桑托州塞拉市野生动物筛选中心(CETAS)圈养的黑耳负鼠行为的影响。本研究建立了两种食物模型:“惊喜管”和“食物谜题”。选取24只幼黑耳负鼠,不分性别,分为8组,每组3只,4组暴露于一种模型,4组暴露于另一种模型。每组接受两种条件:有模型存在的实验组和没有模型存在的对照组。每种情况持续24小时,连续两天发生。它们是用陷阱摄像机拍摄的,产生了3233个视频,每个25秒。为了准备行距图,我们从每组中选择了24个视频,记录在18点到19点30分之间,这段时间动物表现出更活跃。负鼠与这两种模式相互作用,获取和吃隐藏的食物,比起水果,它们更喜欢肉。负鼠的行为与模型没有显著差异,观察到当模型存在时,攻击行为显著下降。我们的结论是,该模型可以作为黑耳负鼠的环境富集,对减少攻击行为有好处。
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引用次数: 0
Uso do Alphitobius diaperinus como método alternativo de osteotécnica 使用尿布Alphitobius作为一种替代的骨技术方法
Pub Date : 2023-01-01 DOI: 10.1590/1809-6891v24e-75530p
Arthur Dalmolin Dahmer, Camila Debastiani Da Silva, Carolina Pietczak, Ana Carolina Gonçalves Dos Reis
Resumo Buscou-se com o presente estudo desenvolver um método alternativo de osteotécnica biológica baseado no uso de cascudinhos (Alphitobius diaperinus), um inseto de comportamento necrófago encontrado nos sistemas de produção avícola. Foram utilizados dez cadáveres de animais de pequeno a médio porte e de diferentes espécies, e duas peças ósseas isoladas. Após a retirada dos tecidos moles, os ossos foram acomodados junto aos insetos até a sua limpeza completa. Na sequência, as peças foram avaliadas, clareadas, secas e os esqueletos montados. A utilização dos cascudinhos na preparação biológica de esqueletos demonstrou resultados bastantes promissores, visto que este inseto é de fácil aquisição, manutenção e manipulação, não emite odores desagradáveis ao ambiente, proporciona uma limpeza completa dos esqueletos, atingindo lugares de difícil acesso. Além disso, apresentam grande agilidade na limpeza das carcaças, sendo dispensável a fixação prévia dos tecidos moles para prevenir a putrefação ou o aparecimento de microrganismos, como colônias de fungos.
摘要本研究旨在开发一种基于cascudinhos (Alphitobius diaperinus)的生物骨技术替代方法,cascudinhos是一种在家禽生产系统中发现的食腐昆虫。使用了10具不同种类的中小型动物尸体和2块分离的骨头。在去除软组织后,骨头被安置在昆虫旁边,直到它们完全清洁。随后,对碎片进行评估、漂白、干燥和组装骨骼。在骨骼的生物制备中使用cascudinhos显示了非常有前景的结果,因为这种昆虫易于获取、维护和操作,不会向环境排放难闻的气味,提供了骨骼的完全清洁,到达难以到达的地方。此外,它们在清洗尸体方面表现出极大的灵活性,无需事先固定软组织,以防止腐烂或微生物的出现,如真菌菌落。
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引用次数: 0
Histomorphological and immunophenotypic diagnoses of gastrointestinal stromal tumors and other sarcomas that affect the intestine of dogs 影响犬肠道的胃肠道间质瘤和其他肉瘤的组织形态学和免疫表型诊断
Pub Date : 2023-01-01 DOI: 10.1590/1809-6891v24e-75610e
Marina Laudares Costa, Felipe Augusto Ruiz Sueiro, Paulo César Jark, Leonardo Lamarca de Carvalho, Marcela Aldrovani Rodrigues, Vinícius Thomaz da Silva Almeida, Fernanda Gosuen Gonçalves Dias
Abstract In view of the morphological similarity between gastrointestinal stromal tumors (GIST) and other sarcomas of the intestine of dogs, the aim was to carry out the histomorphological and immunohistochemical diagnosis of these tumors, associating breed, sex and age, location and tumor invasion. 217 cases were evaluated by histopathology and 36 diagnosed by immunohistochemistry were included (24 GIST and 12 other intestinal sarcomas). Mixed breed dogs were the most diagnosed with GIST, mainly elderly females (9.5±2.2 years); in the other intestinal sarcomas, crossbreeds and Dachshunds, males and females, were equally affected. The cecum was the most affected by GISTs, with tumor invasion of the intestinal layers in all cases. The small intestine was the most affected by the other intestinal sarcomas, with invasion of the layers in most of these tumors. GISTs expressed markers such as CD117 and DOG-1, unlike other intestinal sarcomas. GIST and other intestinal sarcomas denoted histomorphological and immunophenotypic characteristics similar to histopathology, justifying the association of immunohistochemistry for the definitive diagnosis.
摘要针对犬胃肠道间质瘤(GIST)与其他肠道肉瘤在形态上的相似性,结合犬种、性别、年龄、部位及肿瘤侵袭情况,对胃肠道间质瘤进行组织形态学及免疫组化诊断。经组织病理学检查217例,经免疫组化诊断36例(GIST 24例,其他肠肉瘤12例)。胃肠道间质瘤的诊断以杂交犬居多,以高龄犬(9.5±2.2岁)为主;在其他肠道肉瘤中,杂交犬和腊肠犬,雄性和雌性,受到的影响是一样的。盲肠是胃肠道间质瘤的主要受累部位,所有病例均有肿瘤侵袭肠层。小肠受其他肠道肉瘤的影响最大,这些肿瘤大多侵袭肠壁。与其他肠肉瘤不同,gist表达CD117和DOG-1等标志物。胃肠道间质瘤和其他肠肉瘤表现出与组织病理学相似的组织形态学和免疫表型特征,证明了免疫组织化学与最终诊断的相关性。
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引用次数: 0
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Ciência Animal Brasileira
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