甘草叶提取物合成的氧化锰纳米颗粒对乳腺癌细胞株的体外抗癌和抑菌作用

IF 3.8 4区 工程技术 Q2 CHEMISTRY, MULTIDISCIPLINARY Green Processing and Synthesis Pub Date : 2023-01-01 DOI:10.1515/gps-2023-0063
Maryam Musleh Althobiti, Badr Alzahrani, Abozer Y. Elderdery, Nasser A. N. Alzerwi, Musaed Rayzah, Ahmed M. E. Elkhalifa, Bandar Idrees, Ebtisam Bakhsh, Abdulrahim A. Alabdulsalam, A. Mohamedain, Suresh S. Kumar, Pooi Ling Mok
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引用次数: 0

摘要

摘要在本研究中,我们评估了从甘草叶提取物中提取的含pluronic - f127的氧化锰纳米颗粒(pf -127包被Mn 2 O 3 NPs)对乳腺腺癌、MCF7和MDA-MB-231细胞系的抗增殖和凋亡特性。GU叶提取物含有生物活性分子,可作为还原或封盖剂形成mn2o3 NPs。采用各种分析技术表征了pf -127包覆的mn2o3 NPs的理化性质,包括光谱学(超光可见、傅里叶变换红外、光致发光)、电子显微镜(场发射扫描电镜和透射电镜)、x射线衍射(XRD)、电子衍射x射线光谱(EDAX)和动态光散射。NPs的平均晶粒尺寸为80 nm,具有立方晶结构。pf127包封的mn2o3 NPs显著降低MDA-MB-231和MCF-7细胞的增殖,同时增加内源性ROS,降低线粒体基质蛋白水平。DAPI、EtBr/AO双染色和基于annexin - v - fitc的流式细胞术分析显示,pf127包被的Mn 2o3 np处理的乳腺癌细胞表现出核损伤和凋亡细胞死亡,导致细胞周期阻滞在S期。此外,pf127包封的mn2o3 NPs对多种菌株均表现出较强的抗菌效果。因此,我们可以得出结论,pf127包被的mn2o3 NPs可能是有效的未来抗癌药物和乳腺癌的治疗选择。
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In vitro anti-cancer and antimicrobial effects of manganese oxide nanoparticles synthesized using the Glycyrrhiza uralensis leaf extract on breast cancer cell lines
Abstract In this study, we evaluated the antiproliferative and apoptotic properties of Pluronic-F127-containing manganese oxide nanoparticles (PF-127-coated Mn 2 O 3 NPs) derived from the leaf extract of Glycyrrhiza uralensis (GU) on breast adenocarcinoma, MCF7, and MDA-MB-231 cell lines. The leaf extract of GU contains bioactive molecules that act as a reducing or capping agent to form Mn 2 O 3 NPs. Various analytical techniques were used to characterize the physiochemical properties of PF-127-coated Mn 2 O 3 NPs, including spectroscopy (ultralight-Vis, Fourier transform infrared, photoluminescence), electron microscopy (field emission scanning electron microscopy and transmission electron microscopy), X-ray diffraction (XRD), electron diffracted X-ray spectroscopy (EDAX), and dynamic light scattering. The average crystallite size of Mn 2 O 3 NPs was estimated to be 80 nm, and the NPs had a cubic crystalline structure. PF127-encapsulated Mn 2 O 3 NPs significantly reduce MDA-MB-231 and MCF-7 cell proliferation, while increasing endogenous ROS and lowering mitochondrial matrix protein levels. DAPI, EtBr/AO dual staining, and Annexin-V-FITC-based flow cytometry analysis revealed that PF127-coated Mn 2 O 3 NP-treated breast cancer cells exhibit nuclear damage and apoptotic cell death, resulting in cell cycle arrest in the S phase. Furthermore, PF127-encapsulated Mn 2 O 3 NPs show strong antimicrobial efficacy against various strains. As a result, we can conclude that PF127-coated Mn 2 O 3 NPs may be effective as future anticancer agents and treatment options for breast cancer.
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来源期刊
Green Processing and Synthesis
Green Processing and Synthesis CHEMISTRY, MULTIDISCIPLINARY-ENGINEERING, CHEMICAL
CiteScore
6.70
自引率
9.30%
发文量
78
审稿时长
7 weeks
期刊介绍: Green Processing and Synthesis is a bimonthly, peer-reviewed journal that provides up-to-date research both on fundamental as well as applied aspects of innovative green process development and chemical synthesis, giving an appropriate share to industrial views. The contributions are cutting edge, high-impact, authoritative, and provide both pros and cons of potential technologies. Green Processing and Synthesis provides a platform for scientists and engineers, especially chemists and chemical engineers, but is also open for interdisciplinary research from other areas such as physics, materials science, or catalysis.
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