长链非编码RNA MSC-AS1通过调控microRNA-190a-3p促进前列腺癌的侵袭和迁移

IF 2.9 4区 医学 Q1 Medicine Journal of biomedical nanotechnology Pub Date : 2023-10-01 DOI:10.1166/jbn.2023.3683
Qianhao Zhu, Rikao Yu, Renqiang He, Dawei Song, Weihua Liu
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引用次数: 0

摘要

我们通过分析其在56对前列腺癌组织和癌旁组织中的表达,探讨了lncRNA MSC-AS1在前列腺癌(PCa)中的作用。我们研究了MSC-AS1表达、临床病理指标和患者预后之间的关系。在PCa细胞系中,我们过表达或敲低MSC-AS1,并通过transwell试验和伤口愈合试验评估其对细胞功能的影响。我们利用荧光素酶报告基因检测方法探讨了MSC-AS1和miR-190a-3p之间的相互作用。我们的研究结果显示,与邻近组织相比,PCa肿瘤标本中MSC-AS1的表达明显更高。mscs - as1高表达与淋巴结和远处转移的发生率增加相关。过表达MSC-AS1可降低细胞侵袭性和迁移性,而敲除其可增强这些能力。我们观察到miR-190a-3p在PCa组织中的表达降低,与MSC-AS1的表达呈负相关。调节miR-190a-3p表达可抵消MSC-AS1对细胞侵袭性和迁移的影响。总之,我们的研究强调了MSC-AS1与PCa患者转移和不良预后的关联,提示其通过miR-190a-3p调节参与了疾病的恶性进展。MSC-AS1具有作为前列腺癌预后生物标志物和新治疗策略的治疗靶点的潜力。需要进一步的研究来了解潜在的机制,并验证靶向MSC-AS1和miR-190a-3p在PCa治疗中的临床意义。
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Long Chain Non-Coding RNA MSC-AS1 Promote Invasion and Migration of Prostate Cancer Through Regulating microRNA-190a-3p
We investigated the role of the lncRNA MSC-AS1 in prostate cancer (PCa) by analyzing its expression in 56 pairs of PCa tissues and adjacent tissues. We examined the relationship between MSC-AS1 expression, clinicopathological indicators, and patient prognosis. In PCa cell lines, we overexpressed or knocked down MSC-AS1 and assessed its impact on cell function using transwell assays and wound healing tests. We explored the interaction between MSC-AS1 and miR-190a-3p using luciferase reporter assays. Our findings showed significantly higher MSC-AS1 expression in PCa tumor specimens compared to adjacent tissues. High MSC-AS1 expression correlated with increased incidence of lymph node and distant metastasis. Overexpressing MSC-AS1 reduced cell invasiveness and migration, while knocking it down enhanced these abilities. We observed decreased miR-190a-3p expression in PCa tissues, negatively correlating with MSC-AS1 expression. Modulating miR-190a-3p expression counteracted the effects of MSC-AS1 on cell invasiveness and migration. In conclusion, our study highlights the association of MSC-AS1 with metastasis and poor prognosis in PCa patients, suggesting its involvement in the malignant progression of the disease via miR-190a-3p modulation. MSC-AS1 holds potential as a prognostic biomarker for PCa and a therapeutic target for novel treatment strategies. Further research is needed to understand the underlying mechanisms and validate the clinical implications of targeting MSC-AS1 and miR-190a-3p in PCa management.
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CiteScore
4.30
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17.20%
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145
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2.3 months
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